FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490864

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490864
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58993
Sequences flagged as poor quality0
Sequence length301
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA29034.920922821351685No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA19763.3495499466038345No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA19453.297001339141932No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA15142.5664061837845167No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA14652.4833454816673166No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA14272.4189310596172424No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA13652.313833844693438No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA13332.2595901208617972No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA10091.7103724170664316No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA9991.6934212533690436No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA9921.6815554387808722No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA9891.676470089671656No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA9841.6679945078229619No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA9561.6205312494702762No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA9411.5951045039241945No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA9231.5645924092688963No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA7831.3272761175054668No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA7421.2577763463461766No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA7031.1916668079263641No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA6931.1747156442289763No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA6841.1594595969013273No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA6731.1408133168342005No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA6651.1272523858762904No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA6301.067923312935433No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA5770.9780821453392775No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA5230.8865458613733832No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA5200.8814605122641669No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA5050.8560337667180852No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA5000.8475581848693913No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4920.8339972539114809No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA4800.8136558574746156No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA4680.7933144610377502No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA4510.7644974827521909No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA4490.7611072500127134No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA4410.7475463190548032No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA4160.7051684098113336No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3730.6322784059125659No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3640.6170223585849168No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3440.5831200311901412No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA3440.5831200311901412No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3410.5780346820809248No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3400.576339565711186No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3350.5678639838624921No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3040.5153153764005899No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2920.4949739799637245No Hit
GGACTAAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG2680.4542911870899937No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2560.43394979065312833No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2510.42547420880443443No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2480.42038885969521805No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2380.40343769599783025No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2330.39496211414913635No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2290.38818164867018123No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2150.36445001949383826No Hit
GGACTACAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1970.3339379248385402No Hit
GGACTAACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1860.3152916447714136No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGA1850.3135965284016748No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1740.2949502483345482No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1740.2949502483345482No Hit
GGACTAAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1730.2932551319648094No Hit
GGACTAAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA1690.28647466648585423No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1620.2746088518976828No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1580.26782838641872764No Hit
GGACTAAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1570.26613327004898885No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1560.2644381536792501No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1530.25935280457003373No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1490.2525723390910786No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGA1440.24409675724238472No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1370.2322309426542132No Hit
GGACTACCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1370.2322309426542132No Hit
GGACTACAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA1340.22714559354499686No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1290.21867001169630298No Hit
GGACTAAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA1220.20680419710813147No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1210.20510908073839268No Hit
GGACTATAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1210.20510908073839268No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1210.20510908073839268No Hit
GGACTAACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA1210.20510908073839268No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1190.20171884799891512No Hit
GGACTACGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1150.19493838251995999No Hit
GGACTAAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1110.18815791704100487No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1100.18646280067126608No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGA1080.18307256793178853No Hit
GGACTATCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG970.1644262878646619No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGA970.1644262878646619No Hit
GGACTAACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG960.16273117149492314No Hit
GGACTACAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG960.16273117149492314No Hit
GGACTACCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA940.15934093875544555No Hit
GGACTAAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA930.1576458223857068No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGA930.1576458223857068No Hit
GGACTACAAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCA910.1542555896462292No Hit
GGACTATAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA890.15086535690675165No Hit
GGACTAAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA880.14917024053701286No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA860.1457800077975353No Hit
GGACTATGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG850.1440848914277965No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA850.1440848914277965No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA830.14069465868831896No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGA820.13899954231858017No Hit
GGACTACACGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCA820.13899954231858017No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA820.13899954231858017No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGA780.13221907683962505No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA780.13221907683962505No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA770.13052396046988626No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA760.12882884410014747No Hit
GGACTAAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG760.12882884410014747No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA750.1271337277304087No Hit
GGACTACGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA740.12543861136066992No Hit
GGACTAACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA730.12374349499093112No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGA720.12204837862119236No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA690.11696302951197601No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA690.11696302951197601No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA690.11696302951197601No Hit
GGACTACAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA690.11696302951197601No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGA650.11018256403302086No Hit
GGACTACCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG650.11018256403302086No Hit
GGACTATAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG640.10848744766328208No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA630.10679233129354329No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGTC108.4447046E-4295.07
ACTAAGG1300.0295.03
ACTAAAG1950.0295.03
CTATAGG750.0295.04
GGACTAT2200.0295.01
GGACTAA4600.0295.01
ATAGGGT750.0295.06
TATCGGG750.0295.05
ACCCGGG3950.0295.06
AAGGGGT1050.0295.06
CAGGGTC108.4447046E-4295.07
ATCGGGT750.0295.06
GGGACTA108.4447046E-4295.01
ATTTGCC108.4447046E-4295.0295
TACCCGG4000.0295.05
ACAGGGT1950.0295.06
TATAGGG750.0295.05
CTACCCG4000.0295.04
GACTATG750.0295.02
ACTATGG750.0295.03
Produced by FastQC (version 0.11.5)