FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490867

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490867
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47036
Sequences flagged as poor quality0
Sequence length301
%GC46

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCCT206.1223545E-8295.01
TCGCCTA600.0295.02
GTACCCT206.1223545E-8295.01
CAAACCT157.196353E-6295.01
AGATCCT303.6379788E-12295.01
CGAACCT157.196353E-6295.01
ACGCCTA450.0295.02
GATCCTA750.0295.02
ATAGCCT108.439262E-4295.01
CGCGCCT206.1223545E-8295.01
GCGCCTA1050.0295.02
CCTACGA108.439262E-4295.05
CGTTCCT255.2386895E-10295.01
GCACCCT255.2386895E-10295.01
CGCCTAC2750.0295.03
AAGCCTA500.0295.02
TACGCCT157.196353E-6295.01
TGCGCCT206.1223545E-8295.01
GTGTCGG157.196353E-6295.0295
GCTTACG108.439262E-4295.04
Produced by FastQC (version 0.11.5)