FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490869

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490869
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100974
Sequences flagged as poor quality0
Sequence length301
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCCCT350.0295.000031
GTCGCCT350.0295.000031
CTCGGGA700.0295.000036
GAAACCT700.0295.000031
CATACCT350.0295.000031
CTCGCCT350.0295.000031
ACGACCT350.0295.000031
TGATCCT400.0295.01
AGTCCTA1350.0295.02
AGATCCT255.275069E-10295.01
CGAACCT500.0295.01
GTACAGG108.453614E-4295.0295
GCGCGAC108.453614E-4295.0295
GTCCTAC6900.0295.03
AACCACG108.453614E-4295.03
GGTCCTA1900.0295.02
CGCGCCT255.275069E-10295.01
GGATCCT108.453614E-4295.01
CCTACGT108.453614E-4295.05
GCGCCTA1150.0295.02
Produced by FastQC (version 0.11.5)