FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490871

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490871
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81816
Sequences flagged as poor quality0
Sequence length301
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC890.10878067859587366No Hit
CCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC850.10389165933313776No Hit
ATATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC840.10266940451745381No Hit
CTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC840.10266940451745381No Hit
TCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC840.10266940451745381No Hit
ACATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC820.10022489488608584No Hit
ATCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC820.10022489488608584No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCCTA850.0295.000032
GCGCCTA850.0295.000032
TGACCTA850.0295.000032
CCTCCTA1250.0295.000032
GCCCCTA1300.0295.02
GTCGCCT206.150003E-8295.01
CCCCTAC4900.0295.03
TGCCCCT255.275069E-10295.01
AGATCCT350.0295.01
CGAACCT108.4506866E-4295.01
ACGCCTA650.0295.02
TACCACG108.4506866E-4295.03
CAGCCTA1000.0295.02
GAGTCCT108.4506866E-4295.01
ATGGACA108.4506866E-4295.0295
TTGTCCT500.0295.01
GGTCCCT206.150003E-8295.01
GCGCCCT206.150003E-8295.01
TAGCCCT206.150003E-8295.01
AGAACCT206.150003E-8295.01
Produced by FastQC (version 0.11.5)