FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490873

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490873
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72547
Sequences flagged as poor quality0
Sequence length301
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC770.10613808979006714No Hit
TTCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC760.10475967303954677No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACCT350.0295.000031
CATACCT350.0295.000031
CGCACCT350.0295.000031
GGCTCCT350.0295.000031
TGATCCT255.2568794E-10295.01
TCTGCCT157.212473E-6295.01
GCCCCTA800.0295.02
TTGGCCT206.145274E-8295.01
CCCCTAC4050.0295.03
AAATCCT255.2568794E-10295.01
AGATCCT157.212473E-6295.01
CGAACCT108.448706E-4295.01
GCGACCT303.6379788E-12295.01
CCCTCCT255.2568794E-10295.01
TCACCCT255.2568794E-10295.01
CTTCCTA750.0295.02
CACGGGA108.448706E-4295.06
CAGCCTA1000.0295.02
GAGTCCT303.6379788E-12295.01
GGGTCCT206.145274E-8295.01
Produced by FastQC (version 0.11.5)