FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490877

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490877
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73143
Sequences flagged as poor quality0
Sequence length301
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC850.11621071052595601No Hit
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC830.11347634086652175No Hit
TATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC830.11347634086652175No Hit
TTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC800.10937478637737036No Hit
ATAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC790.10800760154765322No Hit
TCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC790.10800760154765322No Hit
TCACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC780.1066404167179361No Hit
ACATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC780.1066404167179361No Hit
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC780.1066404167179361No Hit
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC750.10253886222878471No Hit
TTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC750.10253886222878471No Hit
TTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC740.10117167739906757No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCTA1550.0295.000032
AGGCCTA1100.0295.000032
AGACCTA1100.0295.000032
CAGTCCT550.0295.000031
GACCTAC4400.0295.000033
GTTGAGA108.44885E-4295.0295
TCGCCCT108.44885E-4295.01
GCCCCCT108.44885E-4295.01
ATCCTCG108.44885E-4295.03
AGTCCTA1350.0295.02
GTCGCCT255.2568794E-10295.01
AGTCCCT157.212717E-6295.01
CAAACCT350.0295.01
TGCCCCT157.212717E-6295.01
AGATCCT400.0295.01
ACGCCTA1000.0295.02
TCCGCCT206.1456376E-8295.01
CTACTGG108.44885E-4295.06
GTACAGT108.44885E-4295.0295
CGGCCTA750.0295.02
Produced by FastQC (version 0.11.5)