FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490881

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490881
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71508
Sequences flagged as poor quality0
Sequence length301
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC1010.14124293785310735No Hit
TCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC990.13844604799462995No Hit
CCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC990.13844604799462995No Hit
CTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC950.1328522682776752No Hit
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC950.1328522682776752No Hit
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC940.13145382334843653No Hit
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC930.13005537841919787No Hit
TTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC910.12725848856072047No Hit
CTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC880.12306315377300442No Hit
TATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC880.12306315377300442No Hit
CGATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC880.12306315377300442No Hit
TTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC870.12166470884376573No Hit
TTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC870.12166470884376573No Hit
ACATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC870.12166470884376573No Hit
CACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC860.12026626391452705No Hit
TTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC850.11886781898528835No Hit
CATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC850.11886781898528835No Hit
AAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC840.11746937405604967No Hit
AACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC840.11746937405604967No Hit
ACAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC840.11746937405604967No Hit
ATCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC830.116070929126811No Hit
TACCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC810.11327403926833361No Hit
CACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC810.11327403926833361No Hit
TCACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC810.11327403926833361No Hit
TCGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC810.11327403926833361No Hit
ATGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC810.11327403926833361No Hit
TCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC800.11187559433909493No Hit
ATTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC800.11187559433909493No Hit
TTCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC800.11187559433909493No Hit
CTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC790.11047714940985624No Hit
ACGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC790.11047714940985624No Hit
TTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC780.10907870448061754No Hit
TGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC780.10907870448061754No Hit
GTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC780.10907870448061754No Hit
ATCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC780.10907870448061754No Hit
ATTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC780.10907870448061754No Hit
TACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC770.10768025955137886No Hit
ACTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC770.10768025955137886No Hit
CAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC770.10768025955137886No Hit
TCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC760.10628181462214019No Hit
CTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC760.10628181462214019No Hit
CCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC760.10628181462214019No Hit
AAGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC760.10628181462214019No Hit
AATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC750.1048833696929015No Hit
ATAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC750.1048833696929015No Hit
CTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC750.1048833696929015No Hit
TCTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC750.1048833696929015No Hit
AAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC750.1048833696929015No Hit
CCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC740.1034849247636628No Hit
TGGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC740.1034849247636628No Hit
GCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC740.1034849247636628No Hit
ACCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC740.1034849247636628No Hit
CTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC730.10208647983442412No Hit
TTGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC730.10208647983442412No Hit
CATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC730.10208647983442412No Hit
CTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC730.10208647983442412No Hit
CCACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC720.10068803490518544No Hit
CCCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC720.10068803490518544No Hit
CAGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC720.10068803490518544No Hit
AGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC720.10068803490518544No Hit
TAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC720.10068803490518544No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCCTA1100.0295.000032
TGATCCT303.6379788E-12295.01
GCCCCTA750.0295.02
CCCCTAC3800.0295.03
GTACAGG206.144728E-8295.0295
CGTGCCT157.2120565E-6295.01
CCGGCCT255.2568794E-10295.01
GATCCTA900.0295.02
TTGTCCT500.0295.01
GTCTCCT157.2120565E-6295.01
CGACCTA850.0295.02
GGATCCT206.144728E-8295.01
TGAACCT157.2120565E-6295.01
GCGCCTA650.0295.02
CCTACGA157.2120565E-6295.05
GCGCCCT303.6379788E-12295.01
GTCTACG108.448463E-4295.03
CGCGAAC157.2120565E-6295.0295
CGTTCCT303.6379788E-12295.01
ACGGGGC206.144728E-8295.08
Produced by FastQC (version 0.11.5)