FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490895

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490895
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67248
Sequences flagged as poor quality0
Sequence length301
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC840.12491077801570308No Hit
TTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC820.12193671187247204No Hit
CTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC810.12044967880085652No Hit
TTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC760.11301451344277896No Hit
TCATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC750.11152748037116346No Hit
AAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC750.11152748037116346No Hit
TCCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC730.10855341422793244No Hit
TTTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC710.1055793480847014No Hit
ATCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC710.1055793480847014No Hit
TCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC700.1040923150130859No Hit
ATATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC680.10111824886985486No Hit
CTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC680.10111824886985486No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCCT303.6379788E-12295.01
ATCCCCT206.1419996E-8295.01
AGCCCCT255.2568794E-10295.01
ATCCTAG108.4473327E-4295.03
AGCCTAC3050.0295.03
TTGTCCT350.0295.01
TGGTCCT206.1419996E-8295.01
TCCTCGG206.1419996E-8295.04
AATCCTA850.0295.02
ACGCCTA850.0295.02
CCTACGT108.4473327E-4295.05
GTCACCT157.2101284E-6295.01
CCTACCT255.2568794E-10295.01
ATCTCCT350.0295.01
CACACCT350.0295.01
TGACCTA800.0295.02
TCGACCT206.1419996E-8295.01
CGCCCTA600.0295.02
TTCGCCT206.1419996E-8295.01
CTCCCCT157.2101284E-6295.01
Produced by FastQC (version 0.11.5)