FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490897

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490897
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76252
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACCT550.0295.000031
TCGCCTA1000.0295.02
TACGCCT400.0295.01
CGTACCT206.147275E-8295.01
CGTCCTA1300.0295.02
CGATCCT400.0295.01
CGGACCT255.2568794E-10295.01
TCCTACG21900.0292.979434
CCTACGG72850.0290.545625
CTACGGG70650.0290.198156
GTCCTAC6050.0290.123963
TACGGGA70000.0289.942847
CCCTACG16450.0289.62014
ACGGGAG70250.0288.911048
ACCTACG20600.0288.555824
CGGGAGG69350.0287.555889
GCCTACG14150.0286.660774
GGTCCTA1450.0284.827582
GATCCTA1400.0284.46432
GTACCTA1250.0283.199982
Produced by FastQC (version 0.11.5)