FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490899

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490899
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58678
Sequences flagged as poor quality0
Sequence length301
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCCTA700.0295.000032
CTAGCCT350.0295.000031
TGATCCT400.0295.01
TCGCCCT255.2568794E-10295.01
ACGCCTA750.0295.02
GCGACCT206.1352694E-8295.01
CCTAGGA108.4445946E-4295.05
CCGGCCT255.2568794E-10295.01
CGACCTA900.0295.02
CACGCCT108.4445946E-4295.01
TCGGGCA108.4445946E-4295.0295
TGAACCT206.1352694E-8295.01
CGACCCT255.2568794E-10295.01
CCTACGT108.4445946E-4295.05
CCTACGA206.1352694E-8295.05
CGTTCCT206.1352694E-8295.01
ACCTAGG108.4445946E-4295.04
TGCGCCT255.2568794E-10295.01
CTCGGGA255.2568794E-10295.06
AACGCCT255.2568794E-10295.01
Produced by FastQC (version 0.11.5)