FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490903

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490903
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69731
Sequences flagged as poor quality0
Sequence length301
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCCTA850.0295.02
GCGACCT450.0295.01
CAACGGG157.211278E-6295.06
CGACCTA1300.0295.02
CGCGCCT255.2568794E-10295.01
GCGCCTA650.0295.02
GCGCATA157.211278E-6295.0295
CGCCTAG108.4480067E-4295.03
ACCTAGG303.6379788E-12295.04
TGCGCCT206.143637E-8295.01
CTCGCCT450.0295.01
GGCGCCT108.4480067E-4295.01
CGTCCTA850.0295.02
GCGTCCT157.211278E-6295.01
TCCTACG19100.0293.455474
TACGGGA64000.0291.082037
CCTACGG66900.0291.03145
CGCCTAC3450.0290.724643
CTACGGG65100.0290.46856
ACCTACG18800.0290.292544
Produced by FastQC (version 0.11.5)