FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490905

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490905
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70908
Sequences flagged as poor quality0
Sequence length301
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCCTA850.0295.000032
TCGCCTA900.0295.02
CGAACCT255.2568794E-10295.01
TGACGCC108.4483135E-4295.0295
TGGTTAC108.4483135E-4295.0295
GTTCCCT255.2568794E-10295.01
GGGCCTA1000.0295.02
CCGACCT450.0295.01
GACGCCT108.4483135E-4295.01
GGCGCCT255.2568794E-10295.01
CGTCCTA1000.0295.02
TTCGCCT206.144364E-8295.01
ACGAGGC108.4483135E-4295.08
ATCGCCT400.0295.01
ACGACCT450.0295.01
CGCGCCT157.2118037E-6294.999971
TACGGCG157.2118037E-6294.999977
ACGGGGC157.2118037E-6294.999978
CCGTCCT157.2118037E-6294.999971
CGGGGCA157.2118037E-6294.999979
Produced by FastQC (version 0.11.5)