FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005490911

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490911
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82797
Sequences flagged as poor quality0
Sequence length301
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1220.1473483338768313No Hit
TTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1150.13889392127734096No Hit
ATATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1130.13647837482034375No Hit
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1120.13527060159184512No Hit
TCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1110.1340628283633465No Hit
CTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1100.13285505513484788No Hit
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1090.13164728190634925No Hit
ATTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1080.13043950867785065No Hit
TTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1070.12923173544935204No Hit
ATACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1050.12681618899235478No Hit
TCGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1050.12681618899235478No Hit
ATCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1040.12560841576385617No Hit
TCCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1030.12440064253535757No Hit
CCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1030.12440064253535757No Hit
CTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1030.12440064253535757No Hit
CCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1010.12198509607836032No Hit
CAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1010.12198509607836032No Hit
TCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1010.12198509607836032No Hit
TAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC990.11956954962136308No Hit
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC990.11956954962136308No Hit
TCGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC980.11836177639286448No Hit
CACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC980.11836177639286448No Hit
ACAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC980.11836177639286448No Hit
TATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC970.11715400316436586No Hit
ATAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC960.11594622993586724No Hit
CTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC960.11594622993586724No Hit
AAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC960.11594622993586724No Hit
CATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC960.11594622993586724No Hit
TCCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC950.11473845670736862No Hit
TGATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC950.11473845670736862No Hit
TCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC950.11473845670736862No Hit
ATGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC940.11353068347887002No Hit
CTTCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC930.1123229102503714No Hit
GCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC920.11111513702187277No Hit
ATTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC920.11111513702187277No Hit
ATTCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC910.10990736379337415No Hit
TACGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC910.10990736379337415No Hit
ATGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC910.10990736379337415No Hit
TGTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC910.10990736379337415No Hit
GTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC900.10869959056487553No Hit
AACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC900.10869959056487553No Hit
ACTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC900.10869959056487553No Hit
TATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC890.10749181733637693No Hit
TAGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC890.10749181733637693No Hit
AATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC890.10749181733637693No Hit
GTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC890.10749181733637693No Hit
CCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC890.10749181733637693No Hit
ATCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC890.10749181733637693No Hit
CCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC890.10749181733637693No Hit
CTGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC880.10628404410787831No Hit
GCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC880.10628404410787831No Hit
TCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC880.10628404410787831No Hit
TGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC880.10628404410787831No Hit
TACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC880.10628404410787831No Hit
CGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC870.1050762708793797No Hit
CCTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC870.1050762708793797No Hit
TTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC870.1050762708793797No Hit
TTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC870.1050762708793797No Hit
GTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC860.10386849765088106No Hit
AACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC860.10386849765088106No Hit
CCCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC850.10266072442238244No Hit
GGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC850.10266072442238244No Hit
TTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC850.10266072442238244No Hit
CATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC850.10266072442238244No Hit
ACCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC850.10266072442238244No Hit
TAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC840.10145295119388384No Hit
TGCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC840.10145295119388384No Hit
TGCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC840.10145295119388384No Hit
TAGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC840.10145295119388384No Hit
AGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC840.10145295119388384No Hit
ACGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC840.10145295119388384No Hit
TTCGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC840.10145295119388384No Hit
TCTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC830.10024517796538522No Hit
CGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC830.10024517796538522No Hit
GACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC830.10024517796538522No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)