FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490915

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490915
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52262
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCCT108.44196E-4295.01
TCGGGCA108.44196E-4295.0295
CGCGCCT108.44196E-4295.01
GCGCCCT206.128903E-8295.01
TACGCCT303.6379788E-12295.01
GTGTCGG108.44196E-4295.0295
CGATCCT255.2386895E-10295.01
CGCCCTA650.0295.02
GGCTCCT108.44196E-4295.01
CCTACGG47300.0291.56985
ACGGGAG33450.0291.031378
CTACGGG39900.0289.45496
TACGGGA33900.0289.343667
TCCTACG14250.0288.789464
GCCTACG9450.0288.756624
CCCTACG10750.0286.767464
ACGGTAG7050.0286.63128
CGGGAGG31450.0286.089059
CTACGGT7100.0282.535226
ACCTACG14150.0282.491184
Produced by FastQC (version 0.11.5)