FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490916

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490916
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52262
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACCCGGGTTTCTAATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA2020.38651410202441544Illumina Single End PCR Primer 1 (100% over 32bp)
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1940.3712066128353297No Hit
GGACTACCCGGGTTTCTAATCTCGTATGCCGTCTTCTGCTTGCTGCTGCC1220.23343921013355787RNA PCR Primer, Index 13 (96% over 28bp)
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1140.2181317209444721No Hit
GGACTACCGGGGTTTCTAATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA1050.2009107956067506Illumina Single End PCR Primer 1 (100% over 32bp)
GGACTACCCGGGTTTCTAATCAAGCAGAAGACGGCATACGAGATTTGACT960.1836898702690291TruSeq Adapter, Index 13 (96% over 30bp)
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA950.1817764341203934No Hit
GGACTACCCGGGTTTCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC880.16838238107994336No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA870.16646894493130762No Hit
GGACTACTAGGGTTTCTAATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA820.15690176418812904Illumina Single End PCR Primer 1 (100% over 32bp)
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA820.15690176418812904No Hit
GGACTACCCGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG810.1549883280394933No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA790.15116145574222187No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA740.14159427499904328No Hit
GGACTACAAGGGTTTCTAATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA740.14159427499904328Illumina Single End PCR Primer 1 (100% over 32bp)
GGACTACCGGGGTTTCTAATCTCGTATGCCGTCTTCTGCTTGCTGCTGCC640.12245991351268609RNA PCR Primer, Index 13 (96% over 28bp)
GGACTACTCGGGTTTCTAATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA620.11863304121541465Illumina Single End PCR Primer 1 (100% over 32bp)
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA610.11671960506677893No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA540.10332555202632888No Hit
GGACTACCCGGGTATCTAATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA540.10332555202632888Illumina Single End PCR Primer 1 (100% over 32bp)
GGACTACCCGGGTTTCTTTACCTCCTGCTGGGCAGCCCTCTTATTCAGAT540.10332555202632888No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA530.10141211587769317No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTCG206.128903E-8295.09
GGAGCCT157.200955E-6295.01
GGACTCC1500.0295.01
ACCCGGG7900.0295.06
GGACTAC38450.0293.46551
CCGGGTT5300.0292.216988
CTACCGG4000.0291.31254
CGGGTAT3800.0291.11849
ACTCGGG3300.0290.53036
CGGGTTT8800.0289.97169
TACCCGG8150.0289.570565
CCCGGGT7650.0289.215677
GACTACC25300.0288.586942
TCGGGTT1950.0287.43598
CTACCCG13350.0286.161044
TACCGGG4050.0284.074075
CCGGGTA2150.0281.279088
CTAGGGT3900.0279.87187
TACCCGT5250.0278.142855
TACTGGG1750.0278.142855
Produced by FastQC (version 0.11.5)