FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490919

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490919
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71399
Sequences flagged as poor quality0
Sequence length301
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1320.18487653888709926No Hit
TCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1320.18487653888709926No Hit
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1290.1806747993669379No Hit
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1230.1722713203266152No Hit
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1200.16806958080645387No Hit
CAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1170.16386784128629253No Hit
CACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1160.16246726144623874No Hit
TAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1140.15966610176613116No Hit
TAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1140.15966610176613116No Hit
ACAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1140.15966610176613116No Hit
ATAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1120.1568649420860236No Hit
CTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1080.15126262272580848No Hit
GTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1070.14986204288575472No Hit
CTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1060.14846146304570093No Hit
TCGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1060.14846146304570093No Hit
ATATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1050.14706088320564714No Hit
ACATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1040.14566030336559335No Hit
TACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1040.14566030336559335No Hit
TTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1020.1428591436854858No Hit
TTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1020.1428591436854858No Hit
TCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1000.14005798400537822No Hit
AAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC990.13865740416532443No Hit
CTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC980.13725682432527067No Hit
ACCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC980.13725682432527067No Hit
CTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC980.13725682432527067No Hit
CCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC970.13585624448521688No Hit
TGTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC970.13585624448521688No Hit
CAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC960.1344556646451631No Hit
TTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC960.1344556646451631No Hit
AAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC960.1344556646451631No Hit
GTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC950.1330550848051093No Hit
TCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC950.1330550848051093No Hit
ACTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC950.1330550848051093No Hit
TTTCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC950.1330550848051093No Hit
GTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC940.13165450496505554No Hit
TCCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC940.13165450496505554No Hit
AACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC940.13165450496505554No Hit
TTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC940.13165450496505554No Hit
TACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC930.13025392512500175No Hit
CCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC920.12885334528494796No Hit
CTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC920.12885334528494796No Hit
TTAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC920.12885334528494796No Hit
GATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC920.12885334528494796No Hit
ACCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC920.12885334528494796No Hit
TTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC920.12885334528494796No Hit
CCCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC910.12745276544489417No Hit
TGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC910.12745276544489417No Hit
ACGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC910.12745276544489417No Hit
GTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC910.12745276544489417No Hit
ATCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC910.12745276544489417No Hit
ATTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC910.12745276544489417No Hit
AAGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC910.12745276544489417No Hit
CGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC900.12605218560484038No Hit
TGGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC900.12605218560484038No Hit
CACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC900.12605218560484038No Hit
TCACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC900.12605218560484038No Hit
TGAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC900.12605218560484038No Hit
TTGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC890.12465160576478661No Hit
TGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC890.12465160576478661No Hit
TCGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC890.12465160576478661No Hit
CCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC880.12325102592473285No Hit
CTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC880.12325102592473285No Hit
ATGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC880.12325102592473285No Hit
TATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC880.12325102592473285No Hit
GGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC870.12185044608467907No Hit
TGATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC870.12185044608467907No Hit
ATCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC870.12185044608467907No Hit
TTCGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC870.12185044608467907No Hit
TGCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC870.12185044608467907No Hit
ATCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC860.12044986624462528No Hit
TTCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC860.12044986624462528No Hit
CTAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC860.12044986624462528No Hit
AGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC860.12044986624462528No Hit
AGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC850.1190492864045715No Hit
CAACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC850.1190492864045715No Hit
CGAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC850.1190492864045715No Hit
AAACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC850.1190492864045715No Hit
TCTCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC840.11764870656451772No Hit
ATTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC840.11764870656451772No Hit
AATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC840.11764870656451772No Hit
ATGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC830.11624812672446393No Hit
TCTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC830.11624812672446393No Hit
TAGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC830.11624812672446393No Hit
CCCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC830.11624812672446393No Hit
CATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC830.11624812672446393No Hit
TCCGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC820.11484754688441015No Hit
CTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC820.11484754688441015No Hit
ACTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC820.11484754688441015No Hit
GGAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC820.11484754688441015No Hit
TCCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC810.11344696704435636No Hit
CTGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC810.11344696704435636No Hit
AACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC810.11344696704435636No Hit
ACCGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC810.11344696704435636No Hit
TATCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC800.11204638720430259No Hit
ATAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC800.11204638720430259No Hit
GGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC800.11204638720430259No Hit
GACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC800.11204638720430259No Hit
CCAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC800.11204638720430259No Hit
CGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC790.1106458073642488No Hit
GCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC790.1106458073642488No Hit
GTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC790.1106458073642488No Hit
ATGGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC790.1106458073642488No Hit
GTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC790.1106458073642488No Hit
GTGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC790.1106458073642488No Hit
GAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC790.1106458073642488No Hit
GTCGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC780.10924522752419502No Hit
TCGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC780.10924522752419502No Hit
CATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC780.10924522752419502No Hit
ACACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC770.10784464768414123No Hit
CGATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC770.10784464768414123No Hit
AATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC770.10784464768414123No Hit
ACCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC770.10784464768414123No Hit
GTGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC760.10644406784408744No Hit
AGTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC760.10644406784408744No Hit
GGATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC760.10644406784408744No Hit
TAGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC760.10644406784408744No Hit
ATTGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC760.10644406784408744No Hit
TACCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC750.10504348800403367No Hit
AGAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC750.10504348800403367No Hit
AAAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC750.10504348800403367No Hit
CCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC750.10504348800403367No Hit
CGGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC750.10504348800403367No Hit
CGGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC740.10364290816397988No Hit
TGGGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC740.10364290816397988No Hit
AGACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC740.10364290816397988No Hit
CCGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC740.10364290816397988No Hit
CGCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC740.10364290816397988No Hit
AGATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC740.10364290816397988No Hit
CATCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC730.1022423283239261No Hit
GGGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC730.1022423283239261No Hit
GCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC730.1022423283239261No Hit
ATCGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC730.1022423283239261No Hit
CCACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC730.1022423283239261No Hit
TATGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC730.1022423283239261No Hit
TAAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC720.10084174848387231No Hit
CTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC720.10084174848387231No Hit
ATGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC720.10084174848387231No Hit
ACGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC720.10084174848387231No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCT550.0295.000031
GCCCCTA900.0295.02
ACGCCTA750.0295.02
GAGTCCT303.6379788E-12295.01
TTGTCCT400.0295.01
CACGCCT450.0295.01
CGCGCCT206.144546E-8295.01
GGATCCT157.211993E-6295.01
CCTACGT108.4484264E-4295.05
CCTACGA255.2568794E-10295.05
GCACCCT255.2568794E-10295.01
CGCCTCG108.4484264E-4295.03
CGGGGCA157.211993E-6295.09
AAGCCTA1000.0295.02
AATGCCT255.2568794E-10295.01
GTAGCCT206.144546E-8295.01
ACCTAGG108.4484264E-4295.04
TACGCCT157.211993E-6295.01
TATTCCT303.6379788E-12295.01
TCGTCCT400.0295.01
Produced by FastQC (version 0.11.5)