FastQCFastQC Report
Wed 8 May 2024
EGAF00005490921

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490921
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63201
Total Bases19 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1070.16930111865318587No Hit
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1030.16297210487175837No Hit
TAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1020.16138985142640147No Hit
TCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1010.1598075979810446No Hit
TACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC990.15664309109033084No Hit
ATATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC970.1534785841996171No Hit
TTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC970.1534785841996171No Hit
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC960.1518963307542602No Hit
TACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC950.15031407730890334No Hit
CCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC930.1471495704181896No Hit
ACATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC920.1455673169728327No Hit
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC920.1455673169728327No Hit
CCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC910.14398506352747584No Hit
ACAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC910.14398506352747584No Hit
ATCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC910.14398506352747584No Hit
TTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC890.14082055663676207No Hit
AAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC880.1392383031914052No Hit
ATGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC880.1392383031914052No Hit
TAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC880.1392383031914052No Hit
ATTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC880.1392383031914052No Hit
CTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC870.1376560497460483No Hit
CAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC870.1376560497460483No Hit
TCGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC870.1376560497460483No Hit
TTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC860.13607379630069144No Hit
CTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC860.13607379630069144No Hit
TCACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC860.13607379630069144No Hit
CTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC850.13449154285533457No Hit
ATCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC850.13449154285533457No Hit
TATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC850.13449154285533457No Hit
TCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC840.13290928940997768No Hit
CAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC840.13290928940997768No Hit
TAGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC840.13290928940997768No Hit
AACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC840.13290928940997768No Hit
TCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC840.13290928940997768No Hit
GTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC830.1313270359646208No Hit
TTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC830.1313270359646208No Hit
TATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC820.12974478251926394No Hit
TCCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC820.12974478251926394No Hit
TTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC820.12974478251926394No Hit
CATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC820.12974478251926394No Hit
CCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC820.12974478251926394No Hit
TCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC810.12816252907390707No Hit
AACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC810.12816252907390707No Hit
CGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC800.12658027562855018No Hit
TGCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC800.12658027562855018No Hit
ACTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC790.1249980221831933No Hit
TCGGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC790.1249980221831933No Hit
ATAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC790.1249980221831933No Hit
TAACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC790.1249980221831933No Hit
CTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC780.12341576873783644No Hit
CTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC780.12341576873783644No Hit
GACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC770.12183351529247956No Hit
GCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC770.12183351529247956No Hit
AGAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC760.12025126184712268No Hit
GTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC760.12025126184712268No Hit
AAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC760.12025126184712268No Hit
ACGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC760.12025126184712268No Hit
CTGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC760.12025126184712268No Hit
TTGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC760.12025126184712268No Hit
CACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC750.11866900840176578No Hit
TGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC750.11866900840176578No Hit
TCTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC750.11866900840176578No Hit
AAAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC750.11866900840176578No Hit
GTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC750.11866900840176578No Hit
TCAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC750.11866900840176578No Hit
CCTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC740.11708675495640893No Hit
TTCGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC740.11708675495640893No Hit
TCCGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC730.11550450151105204No Hit
AAGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC730.11550450151105204No Hit
TTAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC730.11550450151105204No Hit
AATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC730.11550450151105204No Hit
ATGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC730.11550450151105204No Hit
TTGGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC720.11392224806569516No Hit
GGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC720.11392224806569516No Hit
CGATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC720.11392224806569516No Hit
TGATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC720.11392224806569516No Hit
ATTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC720.11392224806569516No Hit
CTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC720.11392224806569516No Hit
AAGGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC710.11233999462033828No Hit
AGGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC710.11233999462033828No Hit
TGGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC710.11233999462033828No Hit
TGACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC710.11233999462033828No Hit
TTTGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC710.11233999462033828No Hit
TACCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC700.11075774117498141No Hit
GTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC700.11075774117498141No Hit
AATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC700.11075774117498141No Hit
TCCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC700.11075774117498141No Hit
TAGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC700.11075774117498141No Hit
AGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC690.10917548772962453No Hit
ATACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC690.10917548772962453No Hit
GTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC690.10917548772962453No Hit
TTTCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC690.10917548772962453No Hit
CCGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC690.10917548772962453No Hit
AGATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC690.10917548772962453No Hit
AACCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC680.10759323428426765No Hit
CAGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC680.10759323428426765No Hit
CGACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC680.10759323428426765No Hit
ACTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC680.10759323428426765No Hit
ATGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC680.10759323428426765No Hit
TGGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC680.10759323428426765No Hit
CCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC680.10759323428426765No Hit
TGTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC680.10759323428426765No Hit
ACCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC680.10759323428426765No Hit
TCGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC680.10759323428426765No Hit
CACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC670.10601098083891079No Hit
GGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC670.10601098083891079No Hit
ACAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC670.10601098083891079No Hit
CATCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC670.10601098083891079No Hit
CCCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC670.10601098083891079No Hit
CACCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC660.10442872739355391No Hit
ATGGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC660.10442872739355391No Hit
CAGGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC660.10442872739355391No Hit
GAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC660.10442872739355391No Hit
GAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC660.10442872739355391No Hit
ACACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC650.10284647394819701No Hit
TATCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC650.10284647394819701No Hit
TTCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC640.10126422050284013No Hit
CTCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC640.10126422050284013No Hit
GTCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC640.10126422050284013No Hit
CCAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC640.10126422050284013No Hit
CGCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC640.10126422050284013No Hit
CCGGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC640.10126422050284013No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)