FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490927

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490927
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72128
Sequences flagged as poor quality0
Sequence length301
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGATT255.2568794E-10295.0295
GCCCCTA750.0295.02
GCCCCCT303.6379788E-12295.01
GTGCAGT206.145092E-8295.0295
GCGACTT108.448609E-4295.06
GTACAGT108.448609E-4295.0295
TACCACG108.448609E-4295.03
CACGGGA303.6379788E-12295.06
CCTAGGA108.448609E-4295.05
GAGTCCT206.145092E-8295.01
GGGTCCT157.2123075E-6295.01
ACCGCCT108.448609E-4295.01
ATTACCT255.2568794E-10295.01
TTGTCCT600.0295.01
GATCCCT157.2123075E-6295.01
AGGACCT350.0295.01
CGCGCCT255.2568794E-10295.01
TGAACCT303.6379788E-12295.01
GCGCCTA800.0295.02
CCTACGA157.2123075E-6295.05
Produced by FastQC (version 0.11.5)