FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490929

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490929
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79605
Sequences flagged as poor quality0
Sequence length301
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCTA1550.0295.000032
TTGCCTA1100.0295.000032
TTTTCCT550.0295.000031
GTGCCTA550.0295.000032
TGATCCT450.0295.01
GCCCCTA950.0295.02
GTCGCCT157.215125E-6295.01
CCCCTAC3650.0295.03
CAAACCT255.275069E-10295.01
AGATCCT450.0295.01
CGAACCT157.215125E-6295.01
CCCTCCT303.6379788E-12295.01
CAGCCTA750.0295.02
AGCCCTA1050.0295.02
GAGTCCT255.275069E-10295.01
GCGCGAA500.0295.0295
TCTTCCT450.0295.01
GTCCTAC5100.0295.03
ATTACCT350.0295.01
CTCCCTA950.0295.02
Produced by FastQC (version 0.11.5)