FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490931

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490931
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45878
Sequences flagged as poor quality0
Sequence length301
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC690.15039888399668686No Hit
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC680.1482191900257204No Hit
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC670.14603949605475391No Hit
CTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC670.14603949605475391No Hit
ATATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC660.14385980208378743No Hit
TCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC640.1395004141418545No Hit
ATAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC630.137320720170888No Hit
TCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC630.137320720170888No Hit
ATCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC620.13514102619992152No Hit
TTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC620.13514102619992152No Hit
AATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC610.13296133222895506No Hit
CCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC610.13296133222895506No Hit
TAACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC610.13296133222895506No Hit
TTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC600.13078163825798858No Hit
TTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC600.13078163825798858No Hit
ACATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC590.1286019442870221No Hit
TGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC580.12642225031605564No Hit
TGGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC580.12642225031605564No Hit
TCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC570.12424255634508916No Hit
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC570.12424255634508916No Hit
AACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC570.12424255634508916No Hit
TTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC570.12424255634508916No Hit
CTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC570.12424255634508916No Hit
CGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC560.12206286237412267No Hit
TCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC560.12206286237412267No Hit
CTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC550.11988316840315619No Hit
CTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC550.11988316840315619No Hit
CTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC540.11770347443218973No Hit
TAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC540.11770347443218973No Hit
AGGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC540.11770347443218973No Hit
CTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC540.11770347443218973No Hit
ATGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC540.11770347443218973No Hit
ATCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC540.11770347443218973No Hit
AAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC530.11552378046122325No Hit
AGCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC530.11552378046122325No Hit
GAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC530.11552378046122325No Hit
ACCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC530.11552378046122325No Hit
ACGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC520.11334408649025676No Hit
TATCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC520.11334408649025676No Hit
CACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC520.11334408649025676No Hit
GTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC520.11334408649025676No Hit
GGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC510.11116439251929028No Hit
GTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC510.11116439251929028No Hit
TTCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC510.11116439251929028No Hit
TATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC510.11116439251929028No Hit
GTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC510.11116439251929028No Hit
GCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC510.11116439251929028No Hit
TCACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC510.11116439251929028No Hit
CAGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC510.11116439251929028No Hit
TAGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC510.11116439251929028No Hit
ACAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC510.11116439251929028No Hit
GATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC510.11116439251929028No Hit
TTGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC500.10898469854832382No Hit
TTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC500.10898469854832382No Hit
CACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC500.10898469854832382No Hit
GTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC500.10898469854832382No Hit
TGAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC500.10898469854832382No Hit
CAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC500.10898469854832382No Hit
CCCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC500.10898469854832382No Hit
TCCGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC490.10680500457735734No Hit
TACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC490.10680500457735734No Hit
TTAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC490.10680500457735734No Hit
TATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC490.10680500457735734No Hit
CCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC490.10680500457735734No Hit
TACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC490.10680500457735734No Hit
AGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC480.10462531060639085No Hit
TCAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC480.10462531060639085No Hit
TAGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC480.10462531060639085No Hit
CCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC470.1024456166354244No Hit
CACCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC470.1024456166354244No Hit
ACTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC470.1024456166354244No Hit
TCGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC470.1024456166354244No Hit
GGCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC470.1024456166354244No Hit
CAGGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC470.1024456166354244No Hit
TCCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC470.1024456166354244No Hit
TGCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC470.1024456166354244No Hit
GCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC470.1024456166354244No Hit
ACTCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC470.1024456166354244No Hit
TTTCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC470.1024456166354244No Hit
ACACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC470.1024456166354244No Hit
GTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC470.1024456166354244No Hit
TGATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC470.1024456166354244No Hit
GAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC460.10026592266445791No Hit
GGTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC460.10026592266445791No Hit
AGGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC460.10026592266445791No Hit
AGTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC460.10026592266445791No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCTAC1700.0295.000033
AAACCTA950.0295.000032
TTACCTA850.0295.000032
CCGCCTA850.0295.000032
GAACCTA950.0295.000032
TCACCTA850.0295.000032
CCCCCTA850.0295.000032
AGCCCTA850.0295.000032
CGTTCCT206.1207174E-8295.01
ATCCCCT206.1207174E-8295.01
GTATGTT206.1207174E-8295.0295
TGGCATA108.438588E-4295.0295
TTGTCCT206.1207174E-8295.01
TGGTCCT108.438588E-4295.01
AATCCTA750.0295.02
ACGCCTA500.0295.02
GGGTCCT206.1207174E-8295.01
GTCACCT108.438588E-4295.01
CACACCT255.2386895E-10295.01
TATACCT108.438588E-4295.01
Produced by FastQC (version 0.11.5)