FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490933

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490933
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121135
Sequences flagged as poor quality0
Sequence length301
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1870.1543732199612003No Hit
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1850.15272216948033188No Hit
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1810.14942006851859496No Hit
TTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1740.14364139183555538No Hit
ATATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1550.12795641226730506No Hit
TACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1550.12795641226730506No Hit
AAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1530.12630536178643662No Hit
TCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1510.12465431130556817No Hit
TTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1510.12465431130556817No Hit
TCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1490.12300326082469971No Hit
ATCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1480.12217773558426549No Hit
CAGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1470.12135221034383127No Hit
TCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1470.12135221034383127No Hit
CTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1440.11887563462252859No Hit
CACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1430.11805010938209437No Hit
CCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1420.11722458414166012No Hit
ATGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1410.1163990589012259No Hit
CTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1400.11557353366079169No Hit
TATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1400.11557353366079169No Hit
CTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1390.11474800842035744No Hit
TCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1390.11474800842035744No Hit
ACATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1380.11392248317992322No Hit
CTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1380.11392248317992322No Hit
AACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1380.11392248317992322No Hit
ACCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1380.11392248317992322No Hit
TAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1380.11392248317992322No Hit
AACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1380.11392248317992322No Hit
TTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1380.11392248317992322No Hit
ATAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1370.113096957939489No Hit
GTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1370.113096957939489No Hit
GTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1370.113096957939489No Hit
TGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1360.11227143269905479No Hit
TAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1350.11144590745862054No Hit
TCTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1350.11144590745862054No Hit
GTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1350.11144590745862054No Hit
TCCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1350.11144590745862054No Hit
TACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1350.11144590745862054No Hit
ATCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1350.11144590745862054No Hit
TTTCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1350.11144590745862054No Hit
GTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1340.11062038221818632No Hit
CCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1340.11062038221818632No Hit
GTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1330.1097948569777521No Hit
GTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1330.1097948569777521No Hit
TTGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1320.10896933173731786No Hit
CACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1320.10896933173731786No Hit
ATACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1310.10814380649688364No Hit
ACAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1310.10814380649688364No Hit
AATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1300.10731828125644942No Hit
TCGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1300.10731828125644942No Hit
ATTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1300.10731828125644942No Hit
TACCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1300.10731828125644942No Hit
TAACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1290.1064927560160152No Hit
CAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1290.1064927560160152No Hit
TTGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1280.10566723077558096No Hit
ACACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1280.10566723077558096No Hit
CTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1280.10566723077558096No Hit
CTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1280.10566723077558096No Hit
GTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1280.10566723077558096No Hit
TTCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1270.10484170553514674No Hit
ATTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1260.10401618029471252No Hit
CGATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1260.10401618029471252No Hit
TAGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1250.10319065505427828No Hit
CTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1250.10319065505427828No Hit
TTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1240.10236512981384406No Hit
CCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1230.10153960457340984No Hit
TGATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1230.10153960457340984No Hit
CTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1220.10071407933297559No Hit
CATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1220.10071407933297559No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGGA850.0295.000036
ACCCCTA1700.0295.000032
CGTTCCT600.0295.01
TTGTCCT650.0295.01
TCCTCGG206.1621904E-8295.04
AATCCTT108.455695E-4295.02
ACCGGAG108.455695E-4295.08
ACGCCTA1250.0295.02
TGCACCT750.0295.01
CCGGAGG108.455695E-4295.09
GCCTCCT350.0295.01
ACGCCAC108.455695E-4295.02
GTCACCT600.0295.01
CCTACCT350.0295.01
ATCTCCT600.0295.01
CACACCT600.0295.01
CGCCCTA2300.0295.02
TTTCCTA2550.0295.02
TTCGCCT157.224411E-6295.01
TGTCCTA1800.0295.02
Produced by FastQC (version 0.11.5)