FastQCFastQC Report
Wed 8 May 2024
EGAF00005490937

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490937
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87629
Total Bases26.3 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAACCTACGGGAGGCAGCAGGGGTTAGGTATGACCTTGCTCCCAACCAA980.11183512307569411No Hit
TCCACCTACGGGAGGCAGCAGGGGTTAGGTATGACCTTGCTCCCAACCAA960.10955277362516974No Hit
TTCACCTACGGGAGGCAGCAGGGGTTAGGTATGACCTTGCTCCCAACCAA950.10841159889990756No Hit
AAATCCTACGGGAGGCAGCAGGGGTTAGGTATGACCTTGCTCCCAACCAA930.10612924944938319No Hit
CTATCCTACGGGAGGCAGCAGGGGTTAGGTATGACCTTGCTCCCAACCAA900.10270572527359663No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)