FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490937

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490937
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87629
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAACCTACGGGAGGCAGCAGGGGTTAGGTATGACCTTGCTCCCAACCAA980.11183512307569411No Hit
TCCACCTACGGGAGGCAGCAGGGGTTAGGTATGACCTTGCTCCCAACCAA960.10955277362516974No Hit
TTCACCTACGGGAGGCAGCAGGGGTTAGGTATGACCTTGCTCCCAACCAA950.10841159889990756No Hit
AAATCCTACGGGAGGCAGCAGGGGTTAGGTATGACCTTGCTCCCAACCAA930.10612924944938319No Hit
CTATCCTACGGGAGGCAGCAGGGGTTAGGTATGACCTTGCTCCCAACCAA900.10270572527359663No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATCCT550.0295.000031
GTACCCT255.275069E-10295.01
TGCCCTA1050.0295.02
GCGCGAA400.0295.0295
GTCCTCG108.4517075E-4295.03
GATCCTA1450.0295.02
TCCTCGG255.275069E-10295.04
TTCCCTA1250.0295.02
GTAGCCT255.275069E-10295.01
ACCTAGG157.217599E-6295.04
ACAGCCT450.0295.01
TACCTAG108.4517075E-4295.03
ACGCTTT157.217599E-6295.0295
GTTCCCT255.275069E-10295.01
GAGCCCT255.275069E-10295.01
CGAGCCT255.275069E-10295.01
CCGACCT350.0295.01
GACGCCT157.217599E-6295.01
CGGTCCT450.0295.01
GCTACGG108.4517075E-4295.04
Produced by FastQC (version 0.11.5)