FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490938

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490938
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87629
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACAAGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT22242.537972588983099No Hit
GGACTACAAGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT19582.234420112063358No Hit
GGACTACACGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT13971.5942210911912724No Hit
GGACTACACGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT11731.338597952732543No Hit
GGACTACTAGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT11261.2849627406452202No Hit
GGACTACTAGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT10571.2062216846021294No Hit
GGACTACAGGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT9111.0396101747138504No Hit
GGACTACAGGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT8480.9677161670223329No Hit
GGACTACCAGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT8050.9186456538360588No Hit
GGACTACAAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC7660.8741398395508337No Hit
GGACTACTCGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT7660.8741398395508337No Hit
GGACTACAAGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA7480.8535986944961143No Hit
GGACTACAAGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC6680.7623047164751395No Hit
GGACTACAAGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA6300.7189400769151765No Hit
GGACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC6130.6995401065857193No Hit
GGACTACCAGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT5960.6801401362562621No Hit
GGACTACTCGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT5800.6618813406520673No Hit
GGACTACAAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC5690.6493284186741832No Hit
GGACTACAAGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG5640.6436225450478723No Hit
GGACTACCCGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT5600.6390578461468235No Hit
GGACTACTGGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT5370.6128108274657933No Hit
GGACTACACGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA5100.5819991098837143No Hit
GGACTACTGGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT4900.5591756153784706No Hit
GGACTACAAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG4900.5591756153784706No Hit
GGACTACCGGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT4660.5317874219721782No Hit
GGACTACACGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC4550.5192344999942942No Hit
GGACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC4400.5021168791153613No Hit
GGACTACACGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA4090.4667404626322336No Hit
GGACTACTAGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA4090.4667404626322336No Hit
GGACTACACGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC4030.4598934142806605No Hit
GGACTACCCGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT3940.44962284175330086No Hit
GGACTACAAGGGTTTCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC3920.44734049230277645No Hit
GGACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC3690.42109347362174626No Hit
GGACTACCGGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT3680.41995229889648406No Hit
GGACTACTAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC3610.4119640758196487No Hit
GGACTACTAGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA3560.4062582021933378No Hit
GGACTACACGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG3560.4062582021933378No Hit
GGACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC3520.4016935032922891No Hit
GGACTACTAGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC3430.3914229307649294No Hit
GGACTACACGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC3370.3845758824133563No Hit
GGACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC3310.3777288340617832No Hit
GGACTACCAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC3290.37544648461125885No Hit
GGACTACAGGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA3220.3674582615344235No Hit
GGACTACTAGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG3010.3434935923039176No Hit
GGACTACACGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2960.3377877186776067No Hit
GGACTACAGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC2940.33550536922708235No Hit
GGACTACAGGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA2940.33550536922708235No Hit
GGACTACCAGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA2910.33208184505129584No Hit
GGACTACTAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2840.3240936219744605No Hit
GGACTACTAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2840.3240936219744605No Hit
GGACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2810.32067009779867395No Hit
GGACTACACGGGTTTCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC2760.314964224172363No Hit
GGACTACTCGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA2680.30583482637026554No Hit
GGACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2550.29099955494185714No Hit
GGACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC2440.2784466329639731No Hit
GGACTACAAGGGTATCTAATCGATCTCGTATGCCGTCTTCTGCTTGCTGC2370.2704584098871378RNA PCR Primer, Index 42 (96% over 32bp)
GGACTACAGGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2360.26931723516187567No Hit
GGACTACCAGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2330.2658937109860891No Hit
GGACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2320.2647525362608269No Hit
GGACTACTCGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA2310.2636113615355647No Hit
GGACTACAGGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2300.26247018681030254No Hit
GGACTACAGGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC2300.26247018681030254No Hit
GGACTACTCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC2210.25219961428294285No Hit
GGACTACCAGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC2200.25105843955768065No Hit
GGACTACAAGGGTATCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC2200.25105843955768065No Hit
GGACTACCAGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA2190.24991726483241847No Hit
GGACTACCCGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA2180.24877609010715632No Hit
GGACTACAGGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2170.2476349153818941No Hit
GGACTACCAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2070.23622316812927224No Hit
GGACTACAAGGGTATCTAATGGCACAGGGGCTGGAAGGATCCCATCTTCA2060.2350819934040101No Hit
GGACTACAAGGGTTTCTAATCGATCTCGTATGCCGTCTTCTGCTTGCTGC2060.2350819934040101RNA PCR Primer, Index 42 (96% over 32bp)
GGACTACTCGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC2040.2327996439534857No Hit
GGACTACCCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC2040.2327996439534857No Hit
GGACTACTCGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2000.228234945052437No Hit
GGACTACTAGGGTTTCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1960.22367024615138822No Hit
GGACTACTGGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1940.2213878967008639No Hit
GGACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1930.2202467219756017No Hit
GGACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1860.21225849889876638No Hit
GGACTACCAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1840.20997614944824203No Hit
GGACTACAAGGGTATCTAATTCCACGTCACTAGAAAGTTCTTGGGCCCCG1820.20769379999771767No Hit
GGACTACTCGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1780.2031291010966689No Hit
GGACTACCGGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1730.197423227470358No Hit
GGACTACCCGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1730.197423227470358No Hit
GGACTACTCGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1720.1962820527450958No Hit
GGACTACTGGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1710.19514087801983362No Hit
GGACTACAGGGGTTTCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1690.19285852856930924No Hit
GGACTACAAGGGTTTCTAATGGCACAGGGGCTGGAAGGATCCCATCTTCA1690.19285852856930924No Hit
GGACTACAAGGGTTTCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC1680.19171735384404706No Hit
GGACTACAAGGGTTTCTAATTCCACGTCACTAGAAAGTTCTTGGGCCCCG1680.19171735384404706No Hit
GGACTACTGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1640.18715265494299832No Hit
GGACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1630.18601148021773614No Hit
GGACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1620.18487030549247396No Hit
GGACTACCAGGGTTTCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1590.1814467813166874No Hit
GGACTACCGGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1540.17574090769037648No Hit
GGACTACTGGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1520.1734585582398521No Hit
GGACTACAAGGGTATCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC1520.1734585582398521No Hit
GGACTACAAGGGTATCTAATGGTCCCCGGAGTTTCTCGGCACAGAGCTCA1500.17117620878932774No Hit
GGACTACACGGGTTTCTAATCGATCTCGTATGCCGTCTTCTGCTTGCTGC1480.16889385933880335RNA PCR Primer, Index 42 (100% over 31bp)
GGACTACCCGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1430.16318798571249243No Hit
GGACTACACGGGTATCTAATGGCACAGGGGCTGGAAGGATCCCATCTTCA1410.16090563626196808No Hit
GGACTACCGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1410.16090563626196808No Hit
GGACTACTGGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1390.1586232868114437No Hit
GGACTACAAGGGTATCTAATGGTCCCTGGAGTTTCTCGGCACAGAGCTCA1360.15519976263565716No Hit
GGACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1360.15519976263565716No Hit
GGACTACTGGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1350.15405858791039498No Hit
GGACTACAAGGGTTTCTAATGGTCCCCGGAGTTTCTCGGCACAGAGCTCA1350.15405858791039498No Hit
GGACTACCGGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1310.1494938890093462No Hit
GGACTACACGGGTTTCTAATTCCACGTCACTAGAAAGTTCTTGGGCCCCG1300.14835271428408403No Hit
GGACTACCCGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1280.14607036483355967No Hit
GGACTACTCGGGTTTCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1270.1449291901082975No Hit
GGACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1270.1449291901082975No Hit
GGACTACTAGGGTATCTAATCGATCTCGTATGCCGTCTTCTGCTTGCTGC1250.1426468406577731RNA PCR Primer, Index 42 (100% over 31bp)
GGACTACTAGGGTATCTAATGGCACAGGGGCTGGAAGGATCCCATCTTCA1250.1426468406577731No Hit
GGACTACCGGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1220.13922331648198655No Hit
GGACTACTAGGGTTTCTAATTCCACGTCACTAGAAAGTTCTTGGGCCCCG1200.1369409670314622No Hit
GGACTACAAGGGTTTCTAATGGTCCCTGGAGTTTCTCGGCACAGAGCTCA1180.13465861758093783No Hit
GGACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1180.13465861758093783No Hit
GGACTACAAGGGTATCTAATGGCTGACAGATTATCCTGTGTGCTTGGAGG1170.13351744285567563No Hit
GGACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1170.13351744285567563No Hit
GGACTACACGGGTATCTAATCGATCTCGTATGCCGTCTTCTGCTTGCTGC1160.13237626813041345RNA PCR Primer, Index 42 (100% over 31bp)
GGACTACCCGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1150.13123509340515127No Hit
GGACTACAAGGGTTTCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC1140.13009391867988906No Hit
GGACTACTGGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1130.12895274395462689No Hit
GGACTACACGGGTATCTAATTCCACGTCACTAGAAAGTTCTTGGGCCCCG1130.12895274395462689No Hit
GGACTACTAGGGTATCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC1120.1278115692293647No Hit
GGACTACTAGGGTTTCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC1110.1266703945041025No Hit
GGACTACACGGGTATCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC1100.12552921977884032No Hit
GGACTACCCGGGTTTCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1070.12210569560305379No Hit
GGACTACACGGGTATCTAATGGTCCCCGGAGTTTCTCGGCACAGAGCTCA1060.12096452087779161No Hit
GGACTACACGGGTTTCTAATGGCACAGGGGCTGGAAGGATCCCATCTTCA1060.12096452087779161No Hit
GGACTACAAGGGTTTCTAACACCGGATGTGCTCTGACTCGCCCACTTCCG1050.1198233461525294No Hit
GGACTACACGGGTTTCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC1030.11754099670200505No Hit
GGACTACTGGGGTTTCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1020.11639982197674285No Hit
GGACTACTAGGGTTTCTAATCGATCTCGTATGCCGTCTTCTGCTTGCTGC1010.11525864725148068RNA PCR Primer, Index 42 (100% over 31bp)
GGACTACTAGGGTATCTAATTCCACGTCACTAGAAAGTTCTTGGGCCCCG1010.11525864725148068No Hit
GGACTACAGGGGTATCTAATGGCACAGGGGCTGGAAGGATCCCATCTTCA1010.11525864725148068No Hit
GGACTACAAGGGTATCTAACACCGGATGTGCTCTGACTCGCCCACTTCCG1010.11525864725148068No Hit
GGACTACAAGGGTATCTAATATGATAACAAACAAGAATCCACCGTTCTTT1010.11525864725148068No Hit
GGACTACTAGGGTATCTAATGGTCCCCGGAGTTTCTCGGCACAGAGCTCA1010.11525864725148068No Hit
GGACTACACGGGTATCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC990.11297629780095629No Hit
GGACTACCCGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG980.11183512307569411No Hit
GGACTACTAGGGTTTCTAATGGCACAGGGGCTGGAAGGATCCCATCTTCA950.10841159889990756No Hit
GGACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC920.10498807472412101No Hit
GGACTACAAGGGTTTCTAATGGCTGACAGATTATCCTGTGTGCTTGGAGG910.10384689999885884No Hit
GGACTACCGGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC900.10270572527359663No Hit
GGACTACAGGGGTTTCTAATGGCACAGGGGCTGGAAGGATCCCATCTTCA880.10042337582307227No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAA108.4518053E-4294.99887295
CGGGGTA2000.0294.998878
ACGGGTA6500.0294.998848
GGACTAC81150.0293.880251
CGGGTAT12650.0293.832869
TACAAGG20700.0293.573765
CAAGGGT20850.0292.876567
ACAAGGG20800.0292.871466
TACTGGG4950.0292.019075
CTACCGG3700.0291.012364
TACTAGG11000.0290.976175
TACCGGG3650.0290.95785
TCGGGTT3100.0290.24088
CACGGGT11900.0290.04097
CCGGGTA2500.0289.098888
GGGGTTT9350.0288.688729
GGGGTAT9350.0288.688729
TACTCGG6900.0288.585855
CTCGGGT6850.0288.539037
CTACTAG11100.0288.354744
Produced by FastQC (version 0.11.5)