FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490943

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490943
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81076
Sequences flagged as poor quality0
Sequence length301
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1060.13074152646899206No Hit
TCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGCCGAGAGGC970.11964083082539839No Hit
TACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC950.11717400957126647No Hit
CTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC920.11347377769006857No Hit
TTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC910.11224036706300262No Hit
CTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC900.11100695643593665No Hit
TAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC900.11100695643593665No Hit
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGCCGAGAGGC880.10854013518180473No Hit
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGCCGAGAGGC870.10730672455473876No Hit
TTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC870.10730672455473876No Hit
TCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC870.10730672455473876No Hit
TCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC860.1060733139276728No Hit
ATAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC860.1060733139276728No Hit
ATTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC860.1060733139276728No Hit
ATATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC850.10483990330060683No Hit
ACCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGCCGAGAGGC850.10483990330060683No Hit
AATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC850.10483990330060683No Hit
TAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC850.10483990330060683No Hit
TACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC840.10360649267354088No Hit
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC840.10360649267354088No Hit
TCCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC830.10237308204647491No Hit
TTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC830.10237308204647491No Hit
ATAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC830.10237308204647491No Hit
ACATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC820.10113967141940894No Hit
TATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGCCGAGAGGC820.10113967141940894No Hit
CTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC820.10113967141940894No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCCCT550.0295.000031
GATCCTA1100.0295.000032
TTGACCT550.0295.000031
CGGCCTA550.0295.000032
ATCGCCT550.0295.000031
AGTTCCT550.0295.000031
CGTTCCT255.275069E-10295.01
ACAGCCT500.0295.01
AGCCTAG206.1496394E-8295.03
TTGTCCT400.0295.01
TGGTCCT303.6379788E-12295.01
AACGCCT206.1496394E-8295.01
ACGCCTA750.0295.02
TGCACCT450.0295.01
GTGTCGG900.0295.0295
CCTACGT108.4505434E-4295.05
CCTACGA350.0295.05
CCTACCT303.6379788E-12295.01
GGCACCT303.6379788E-12295.01
ATCTCCT400.0295.01
Produced by FastQC (version 0.11.5)