FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490944

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490944
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81076
Sequences flagged as poor quality0
Sequence length301
%GC51

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA28983.5744239972371603No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA19742.4347525778282106No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA17832.199171148058612No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA16792.0708964428437513No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA16392.021560017761113No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA16031.9771572351867384No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA15871.9574226651536828No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA15721.9389215057476934No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA11611.4319897380235829No Hit
GGACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA11541.423355863634121No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA11281.391287187330406No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA11001.356751689772559No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA10431.2864472840297994No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA10371.2790468202674035No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA10281.2679461246238097No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA9941.226010163303567No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA9921.2235433420494353No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA9921.2235433420494353No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA9671.192708076372786No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA9411.160639400069071No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA9301.1470718831713456No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA9291.1458384725442794No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA9201.1347377769006857No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA8691.0718338349203218No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA8261.0187971779564853No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA8221.0138635354482215No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA7600.9373920765701318No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA6940.8559869751837782No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA6840.8436528689131185No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA6690.8251517095071291No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA6530.8054171394740737No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA6510.8029503182199418No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA6460.796783265084612No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA6440.7943164438304802No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA6390.7881493906951501No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA6320.7795155163056885No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA6130.7560807143914352No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA5840.7203118062065222No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA5820.7178449849523904No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA5820.7178449849523904No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA5700.7030440574275988No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA5570.6870097192757413No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA5540.6833094873945433No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA5500.6783758448862796No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA5350.6598746854802902No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA5340.6586412748532241No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA5310.6549410429720263No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA5300.6537076323449603No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA5160.6364398835660369No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA5000.6167053135329814No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA4840.596970743499926No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA4800.5920371009916621No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA4570.563668656569145No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA4440.5476343184172875No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA3910.48226355518279146No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA3910.48226355518279146No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA3840.4736296807933297No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA3820.4711628595391978No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA3740.46129557452267006No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA3520.43416054072721894No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA3460.42676007696482315No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA3430.42305984508362526No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3430.42305984508362526No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA3400.4193596132024273No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA3370.4156593813212294No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA3360.41442597069416354No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA3330.41072573881296565No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA3310.40825891755883364No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3240.399625043169372No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA3150.3885243475257783No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA3080.3798904731363165No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3040.3749568306280527No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA3000.37002318811978885No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2990.36878977749272285No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2900.35768908184912923No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2860.3527554393408654No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2850.3515220287137994No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA2710.3342542799348759No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2700.33302086930780994No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2650.32685381617248016No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2590.3194533524100844No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA2570.31698653115595243No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2350.28985149736050125No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2280.2812176229710395No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2180.2688835167003799No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2180.2688835167003799No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA2160.26641669544624796No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA2150.265183284819182No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2100.2590162316838522No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2080.25654941042972024No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1990.24544871478612662No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1960.24174848290492873No Hit
GGACTACAAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCA1930.23804825102373084No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1790.22078050224480736No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1780.21954709161774136No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1760.21708027036360947No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1740.21461344910947752No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1740.21461344910947752No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1690.2084463959741477No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1590.1961122897034881No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA1550.19117864719522423No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1440.17761113029749864No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1370.1689772559080369No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA1340.165277024026839No Hit
GGACTACACGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCA1310.16157679214564113No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1310.16157679214564113No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1280.15787656026444324No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1200.14800927524791552No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1160.14307563273965168No Hit
GGACTACAGGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCA1060.13074152646899206No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA1050.1295081158419261No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1050.1295081158419261No Hit
GGACTACAAGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCA1050.1295081158419261No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1050.1295081158419261No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA1000.12334106270659628No Hit
GGACTACCAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCA980.12087424145246436No Hit
GGACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA970.11964083082539839No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA950.11717400957126647No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA920.11347377769006857No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA900.11100695643593665No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA900.11100695643593665No Hit
GGACTACTAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCA840.10360649267354088No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA820.10113967141940894No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA820.10113967141940894No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACTAC76200.0295.54511
TGACTAC255.2023097E-10295.54511
TGGGGTT2100.0294.998178
GTTTGCC350.0294.99814295
ACCATGG108.450703E-4294.998146
AATGGTT108.450703E-4294.998148
CGGGGTA3500.0294.998148
AGTTGGC157.215791E-6294.99814295
GGGGTAC108.450703E-4294.998149
ACAATGG108.450703E-4294.998146
TGTTAGC108.450703E-4294.99814295
AGTTAGG157.215791E-6294.99814295
CAATGGT108.450703E-4294.998147
AGCTAGC157.215791E-6294.99814295
GGTAGCC108.450703E-4294.99814295
ACTACCC6700.0292.79673
CTACCCG6700.0292.79674
ACGGGTA6650.0292.780128
CAGGGTA6150.0292.59988
TACTGGG5700.0292.410465
Produced by FastQC (version 0.11.5)