FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490948

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490948
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82188
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACACGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG13481.640142113203874No Hit
GGACTACTCGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG12951.5756558135007543No Hit
GGACTACTGGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG10561.2848591035187618No Hit
GGACTACTCGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG9591.1668370078356938No Hit
GGACTACACGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG9061.102350708132574No Hit
GGACTACAGGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG8751.0646323064194285No Hit
GGACTACTAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG8431.025697182070375No Hit
GGACTACACGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT8421.0244804594344674No Hit
GGACTACCCGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG7930.9648610502749794No Hit
GGACTACAAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG7930.9648610502749794No Hit
GGACTACTCGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT7740.9417433201927289No Hit
GGACTACTGGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG6500.790869713340147No Hit
GGACTACTGGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT6300.7665352606219886No Hit
GGACTACTCGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT6270.7628850927142649No Hit
GGACTACCGGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG6090.7409840852679224No Hit
GGACTACAAGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG5750.6996155156470532No Hit
GGACTACCCGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG5620.6837981213802502No Hit
GGACTACACGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT5610.6825813987443422No Hit
GGACTACAGGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG5550.6752810629288947No Hit
GGACTACAGGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT5340.6497298875748284No Hit
GGACTACTAGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG5290.6436462743952889No Hit
GGACTACTAGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT4720.5742930841485374No Hit
GGACTACCGGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG4480.5450917408867474No Hit
GGACTACAAGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT4470.5438750182508395No Hit
GGACTACCCGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT4330.5268409013481288No Hit
GGACTACTGGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT4120.5012897259940624No Hit
GGACTACCAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG3920.476955273275904No Hit
GGACTACTAGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT3590.43680342629094276No Hit
GGACTACAAGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT3570.4343699810191269No Hit
GGACTACCGGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT3560.433153258383219No Hit
GGACTACCCGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT3550.43193653574731106No Hit
GGACTACAGGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT3300.40151846984961304No Hit
GGACTACACGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2690.32729838905923003No Hit
GGACTACCAGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2650.3224314985155984No Hit
GGACTACTCGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2550.3102642721565192No Hit
GGACTACCGGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT2450.29809704579744No Hit
GGACTACACGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA2240.2725458704433737No Hit
GGACTACCAGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT2180.2652455346279262No Hit
GGACTACACGGGTTTCTATCAACTTTAATGGTTCTCTTCCTCCTCCTGCT2110.2567284761765708No Hit
GGACTACTCGGGTTTCTATCAACTTTAATGGTTCTCTTCCTCCTCCTGCT2100.25551175354066286No Hit
GGACTACACGGGTTTCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC2030.24699469508930746No Hit
GGACTACTGGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2030.24699469508930746No Hit
GGACTACCAGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1970.23969435927385993No Hit
GGACTACTCGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1940.2360441913661362No Hit
GGACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1930.23482746873022825No Hit
GGACTACTCGGGTTTCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC1890.22996057818659657No Hit
GGACTACTCGGGTTTCTAATACCTCTTGTCCATGTCTTATGATACGGCCA1880.22874385555068869No Hit
GGACTACACGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1880.22874385555068869No Hit
GGACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1840.223876965007057No Hit
GGACTACACGGGTTTCTAATACCTCTTGTCCATGTCTTATGATACGGCCA1840.223876965007057No Hit
GGACTACTCGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1690.20562612546843823No Hit
GGACTACCCGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1690.20562612546843823No Hit
GGACTACAGGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1680.20440940283253028No Hit
GGACTACACGGGTTTCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC1660.20197595756071446No Hit
GGACTACTCGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1620.19710906701708278No Hit
GGACTACTCGGGTATCTATCAACTTTAATGGTTCTCTTCCTCCTCCTGCT1610.19589234438117487No Hit
GGACTACTGGGGTTTCTATCAACTTTAATGGTTCTCTTCCTCCTCCTGCT1600.19467562174526695No Hit
GGACTACTGGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1550.18859200856572736No Hit
GGACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1500.18250839538618777No Hit
GGACTACACGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1490.18129167275027983No Hit
GGACTACAAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1480.18007495011437194No Hit
GGACTACAGGGGTTTCTATCAACTTTAATGGTTCTCTTCCTCCTCCTGCT1460.17764150484255609No Hit
GGACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1440.17520805957074026No Hit
GGACTACTGGGGTTTCTAATACCTCTTGTCCATGTCTTATGATACGGCCA1430.17399133693483235No Hit
GGACTACAAGGGTTTCTATCAACTTTAATGGTTCTCTTCCTCCTCCTGCT1430.17399133693483235No Hit
GGACTACTAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1400.17034116902710858No Hit
GGACTACTAGGGTTTCTATCAACTTTAATGGTTCTCTTCCTCCTCCTGCT1390.16912444639120067No Hit
GGACTACTCGGGTTTCTAACACCGGATGTGCTCTGACTCGCCCACTTCCG1360.1654742784834769No Hit
GGACTACTCGGGTTTCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC1360.1654742784834769No Hit
GGACTACTGGGGTTTCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC1360.1654742784834769No Hit
GGACTACAGGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1350.164257555847569No Hit
GGACTACACGGGTATCTATCAACTTTAATGGTTCTCTTCCTCCTCCTGCT1350.164257555847569No Hit
GGACTACTCGGGTTTCTAATGGCTGACAGATTATCCTGTGTGCTTGGAGG1340.16304083321166107No Hit
GGACTACCCGGGTTTCTATCAACTTTAATGGTTCTCTTCCTCCTCCTGCT1340.16304083321166107No Hit
GGACTACCCGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1340.16304083321166107No Hit
GGACTACTCGGGTATCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC1320.16060738793984522No Hit
GGACTACAAGGGTTTCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC1310.1593906653039373No Hit
GGACTACACGGGTATCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC1300.1581739426680294No Hit
GGACTACAGGGGTTTCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC1300.1581739426680294No Hit
GGACTACACGGGTTTCTAATGGCTGACAGATTATCCTGTGTGCTTGGAGG1270.15452377476030563No Hit
GGACTACTAGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1260.15330705212439774No Hit
GGACTACACGGGTTTCTAACACCGGATGTGCTCTGACTCGCCCACTTCCG1260.15330705212439774No Hit
GGACTACACGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1230.14965688421667397No Hit
GGACTACACGGGTATCTAATGGCTGACAGATTATCCTGTGTGCTTGGAGG1220.14844016158076606No Hit
GGACTACTCGGGTATCTAATACCTCTTGTCCATGTCTTATGATACGGCCA1220.14844016158076606No Hit
GGACTACAAGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1210.14722343894485812No Hit
GGACTACTCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1200.1460067163089502No Hit
GGACTACTGGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1190.1447899936730423No Hit
GGACTACAGGGGTTTCTAATACCTCTTGTCCATGTCTTATGATACGGCCA1180.14357327103713435No Hit
GGACTACACGGGTATCTAATACCTCTTGTCCATGTCTTATGATACGGCCA1160.14113982576531853No Hit
GGACTACCGGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1150.13992310312941061No Hit
GGACTACTAGGGTTTCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC1150.13992310312941061No Hit
GGACTACACGGGTTTCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1130.1374896578575948No Hit
GGACTACTGGGGTTTCTAATGGCTGACAGATTATCCTGTGTGCTTGGAGG1120.13627293522168685No Hit
GGACTACAAGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1120.13627293522168685No Hit
GGACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1120.13627293522168685No Hit
GGACTACAGGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1120.13627293522168685No Hit
GGACTACAAGGGTATCTATCAACTTTAATGGTTCTCTTCCTCCTCCTGCT1110.13505621258577896No Hit
GGACTACTAGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1100.13383948994987102No Hit
GGACTACTCGGGTATCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC1090.1326227673139631No Hit
GGACTACACGGGTATCTAACACCGGATGTGCTCTGACTCGCCCACTTCCG1090.1326227673139631No Hit
GGACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1090.1326227673139631No Hit
GGACTACTGGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1090.1326227673139631No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1080.1314060446780552No Hit
GGACTACTGGGGTTTCTAACACCGGATGTGCTCTGACTCGCCCACTTCCG1080.1314060446780552No Hit
GGACTACCCGGGTATCTATCAACTTTAATGGTTCTCTTCCTCCTCCTGCT1070.13018932204214725No Hit
GGACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1070.13018932204214725No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1070.13018932204214725No Hit
GGACTACTGGGGTTTCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC1070.13018932204214725No Hit
GGACTACCGGGGTTTCTATCAACTTTAATGGTTCTCTTCCTCCTCCTGCT1060.12897259940623937No Hit
GGACTACACGGGTATCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC1060.12897259940623937No Hit
GGACTACCCGGGTTTCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC1060.12897259940623937No Hit
GGACTACAAGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1040.12653915413442351No Hit
GGACTACAGGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1030.1253224314985156No Hit
GGACTACTCGGGTTTCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1010.12288898622669975No Hit
GGACTACAGGGGTATCTATCAACTTTAATGGTTCTCTTCCTCCTCCTGCT1010.12288898622669975No Hit
GGACTACTGGGGTATCTATCAACTTTAATGGTTCTCTTCCTCCTCCTGCT1000.12167226359079185No Hit
GGACTACCCGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1000.12167226359079185No Hit
GGACTACAGGGGTATCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC1000.12167226359079185No Hit
GGACTACTCGGGTATCTAATGGCTGACAGATTATCCTGTGTGCTTGGAGG1000.12167226359079185No Hit
GGACTACCCGGGTTTCTAACACCGGATGTGCTCTGACTCGCCCACTTCCG980.119238818318976No Hit
GGACTACAGGGGTTTCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC980.119238818318976No Hit
GGACTACACGGGTTTCTAAAAGGCTAGATAGTAACTGTGGAATTTTGCAA980.119238818318976No Hit
GGACTACTGGGGTTTCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC960.11680537304716017No Hit
GGACTACTAGGGTATCTATCAACTTTAATGGTTCTCTTCCTCCTCCTGCT950.11558865041125224No Hit
GGACTACAAGGGTATCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC950.11558865041125224No Hit
GGACTACTAGGGTTTCTAATACCTCTTGTCCATGTCTTATGATACGGCCA930.11315520513943642No Hit
GGACTACCGGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA930.11315520513943642No Hit
GGACTACTAGGGTTTCTAACACCGGATGTGCTCTGACTCGCCCACTTCCG920.1119384825035285No Hit
GGACTACAAGGGTTTCTAATACCTCTTGTCCATGTCTTATGATACGGCCA920.1119384825035285No Hit
GGACTACTAGGGTTTCTAATGGCTGACAGATTATCCTGTGTGCTTGGAGG910.11072175986762058No Hit
GGACTACTGGGGTATCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC900.10950503723171265No Hit
GGACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC900.10950503723171265No Hit
GGACTACCGGGGTTTCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC890.10828831459580475No Hit
GGACTACTGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC880.10707159195989682No Hit
GGACTACTAGGGTTTCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC880.10707159195989682No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA850.10342142405217307No Hit
GGACTACAGGGGTTTCTAATGGCTGACAGATTATCCTGTGTGCTTGGAGG850.10342142405217307No Hit
GGACTACCCGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA840.10220470141626514No Hit
GGACTACCCGGGTTTCTAATGGCTGACAGATTATCCTGTGTGCTTGGAGG830.10098797878035723No Hit
GGACTACTCGGGTATCTAACACCGGATGTGCTCTGACTCGCCCACTTCCG830.10098797878035723No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACTTC108.4507535E-4294.999971
GGACTAC78300.0294.434881
TCGGGTT7500.0293.033338
TACTCGG12450.0291.445775
CGGGTTT20950.0290.775679
GACTACC19400.0289.677832
CTACTCG12600.0289.146824
TACACGG14450.0288.875435
CTACCCG8300.0287.891574
CGGGTAT14100.0287.67739
TACTGGG9700.0287.396885
CACGGGT14550.0286.897
ACGGGTT8800.0286.619328
ACTACCG6950.0286.51083
ACACGGG14600.0284.897286
GACTACA30200.0284.74342
ACGGGTA5700.0284.649148
ACCCGGG8400.0284.46436
CAAGGGT8350.0284.40127
GGGGTAT10050.0283.25879
Produced by FastQC (version 0.11.5)