FastQCFastQC Report
Wed 8 May 2024
EGAF00005490953

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490953
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80922
Total Bases24.3 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1000.12357578903141296No Hit
TCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC950.1173969995798423No Hit
ACAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC920.11368972590889993No Hit
ATCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC880.10874669434764342No Hit
TAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC840.10380366278638689No Hit
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC830.10256790489607276No Hit
CTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC830.10256790489607276No Hit
CAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC830.10256790489607276No Hit
CCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC820.10133214700575864No Hit
TCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC810.10009638911544451No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)