FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490955

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490955
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90289
Sequences flagged as poor quality0
Sequence length301
%GC46

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCCT550.0295.000031
ATAGCCT550.0295.000031
TCGCCTA1200.0295.02
GCGACCT157.218321E-6295.01
CCTAGGA255.275069E-10295.05
GATCCTA1600.0295.02
CGCGCCT206.153641E-8295.01
GCGCCTA950.0295.02
GTTACCT255.275069E-10295.01
TACGCCT450.0295.01
TGCGCCT255.275069E-10295.01
TTACGGG108.4521313E-4295.06
GGCGCCT206.153641E-8295.01
CGCACCT303.6379788E-12295.01
CGTCCTA1650.0295.02
CGATCCT500.0295.01
ATCGCCT400.0295.01
TCCTACG24150.0290.724644
ACCTACG24300.0288.930054
TACGGGA82700.0287.687447
Produced by FastQC (version 0.11.5)