FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490959

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490959
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63334
Sequences flagged as poor quality0
Sequence length301
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1270.20052420500836832No Hit
TTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1140.17999810528310228No Hit
TTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1130.1784191745350049No Hit
TCATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1110.17526131303881012No Hit
TTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1010.15947200555783625No Hit
ATCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC980.15473521331354406No Hit
ATAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC960.15157735181734927No Hit
TCCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC960.15157735181734927No Hit
ACATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC960.15157735181734927No Hit
CTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC950.1499984210692519No Hit
CTTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC940.1484194903211545No Hit
CCATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC940.1484194903211545No Hit
ACCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC930.14684055957305714No Hit
TTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC920.14526162882495974No Hit
ATATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC920.14526162882495974No Hit
AACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC910.14368269807686235No Hit
ACCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC900.14210376732876495No Hit
ACGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC900.14210376732876495No Hit
CCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC900.14210376732876495No Hit
CTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC900.14210376732876495No Hit
TAAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC890.14052483658066758No Hit
ATCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC890.14052483658066758No Hit
TATTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC890.14052483658066758No Hit
TTACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC880.1389459058325702No Hit
TACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC880.1389459058325702No Hit
AAAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC880.1389459058325702No Hit
CCCCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC870.1373669750844728No Hit
ACAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC870.1373669750844728No Hit
CACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC860.1357880443363754No Hit
TCACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC860.1357880443363754No Hit
CAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC860.1357880443363754No Hit
TCTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC860.1357880443363754No Hit
TCTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC860.1357880443363754No Hit
CACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC850.13420911358827803No Hit
AAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC850.13420911358827803No Hit
TCCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC840.13263018284018063No Hit
AACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC840.13263018284018063No Hit
CTCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC830.13105125209208326No Hit
TTTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC830.13105125209208326No Hit
ATGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC820.12947232134398584No Hit
GAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC810.12789339059588847No Hit
ACTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC810.12789339059588847No Hit
GCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC810.12789339059588847No Hit
TAGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC810.12789339059588847No Hit
CTGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC810.12789339059588847No Hit
ATTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC810.12789339059588847No Hit
CATTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC810.12789339059588847No Hit
CCCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC810.12789339059588847No Hit
ACCCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC800.12631445984779108No Hit
CAGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC800.12631445984779108No Hit
TTGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC800.12631445984779108No Hit
ATGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC800.12631445984779108No Hit
CTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC790.1247355290996937No Hit
CCTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC790.1247355290996937No Hit
TTTCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC790.1247355290996937No Hit
GTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC790.1247355290996937No Hit
TAGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC780.12315659835159629No Hit
CAAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC780.12315659835159629No Hit
CATACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC780.12315659835159629No Hit
TCGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC780.12315659835159629No Hit
TCGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC780.12315659835159629No Hit
GTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC770.1215776676034989No Hit
ACTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC770.1215776676034989No Hit
GGGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC760.11999873685540152No Hit
CGAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC760.11999873685540152No Hit
AATACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC760.11999873685540152No Hit
GCGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC750.11841980610730414No Hit
TAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC750.11841980610730414No Hit
TCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC750.11841980610730414No Hit
CGTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC740.11684087535920676No Hit
TTGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC740.11684087535920676No Hit
CGCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC740.11684087535920676No Hit
GGATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC740.11684087535920676No Hit
GGCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC740.11684087535920676No Hit
TGGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC740.11684087535920676No Hit
GCACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC730.11526194461110935No Hit
CTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC730.11526194461110935No Hit
TACCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC730.11526194461110935No Hit
TAACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC730.11526194461110935No Hit
ATTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC720.11368301386301197No Hit
CGTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC720.11368301386301197No Hit
TACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC720.11368301386301197No Hit
TTCCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC720.11368301386301197No Hit
TATGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC710.11210408311491458No Hit
TGAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC710.11210408311491458No Hit
AGCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC710.11210408311491458No Hit
AGGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC710.11210408311491458No Hit
CTGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC710.11210408311491458No Hit
TCCGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC710.11210408311491458No Hit
GTACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC710.11210408311491458No Hit
ATCGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC700.1105251523668172No Hit
GTTCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC700.1105251523668172No Hit
ACTCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC700.1105251523668172No Hit
CATCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC700.1105251523668172No Hit
TCGGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC700.1105251523668172No Hit
CAGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC700.1105251523668172No Hit
ACGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC700.1105251523668172No Hit
TAGCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC700.1105251523668172No Hit
TTAGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC690.1089462216187198No Hit
CACCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC690.1089462216187198No Hit
ACACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC690.1089462216187198No Hit
GGTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC690.1089462216187198No Hit
TGACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC680.10736729087062241No Hit
GGAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC680.10736729087062241No Hit
CAACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC680.10736729087062241No Hit
CCAGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC680.10736729087062241No Hit
TGGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC680.10736729087062241No Hit
ATAGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC680.10736729087062241No Hit
ATACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC670.10578836012252503No Hit
GACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC670.10578836012252503No Hit
TGCCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC670.10578836012252503No Hit
GTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC670.10578836012252503No Hit
AGGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC670.10578836012252503No Hit
ATGGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC670.10578836012252503No Hit
CCGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC670.10578836012252503No Hit
ACCGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC670.10578836012252503No Hit
AATTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC660.10420942937442765No Hit
CTAGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC660.10420942937442765No Hit
GTGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC660.10420942937442765No Hit
GTGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC660.10420942937442765No Hit
TATACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC660.10420942937442765No Hit
GTTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC650.10263049862633027No Hit
GACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC650.10263049862633027No Hit
GGGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC650.10263049862633027No Hit
ATTCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC650.10263049862633027No Hit
TATCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC640.10105156787823286No Hit
TAAGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC640.10105156787823286No Hit
TGCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC640.10105156787823286No Hit
CTCCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC640.10105156787823286No Hit
CGCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC640.10105156787823286No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACCT350.0295.000031
ATCGCCT350.0295.000031
TGTCCTA700.0295.000032
TCGCCTA1100.0295.02
GCCCCTA750.0295.02
TCGCCCT255.2568794E-10295.01
AGTCCTA1000.0295.02
GTCGCCT157.208153E-6295.01
AGATCCT303.6379788E-12295.01
CGAACCT157.208153E-6295.01
GCGACCT157.208153E-6295.01
GTACAGT108.4461767E-4295.0295
CAACGGG108.4461767E-4295.06
CGGCCCT157.208153E-6295.01
GTCCTAC4050.0295.03
CTCCCTA500.0295.02
TTGTCCT206.139089E-8295.01
CGACCTA650.0295.02
AGGACCT400.0295.01
CGCGCCT157.208153E-6295.01
Produced by FastQC (version 0.11.5)