FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490960

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490960
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63334
Sequences flagged as poor quality0
Sequence length301
%GC50

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA13422.118925063946695No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA12902.036820665045631No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA12291.9405058894116904No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA11711.848927906022042No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA11461.809454637319607No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA11171.7636656456247828No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA9711.5331417564025642No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA9001.4210376732876495No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA8901.4052483658066757No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA8751.381564404585215No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA8741.3799854738371176No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA8701.373669750844728No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA8671.3689329586004357No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA8671.3689329586004357No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA8091.2773549752107873No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA8021.2663024599741055No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA8011.2647235292260082No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA7941.2536710139893266No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA7521.187355922569236No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA7461.1778823380806518No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA7371.1636719613477753No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA7331.1573562383553857No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA7311.154198376859191No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA7301.1526194461110937No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA7191.1352512078820223No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA7161.13051441563773No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA6621.0452521552404712No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA6290.9931474405532573No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA6120.9663056178356018No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA5930.9363059336217514No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA5840.9220955568888748No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA5720.9031483879117062No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA5680.8968326649193167No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA5610.885780149682635No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA5590.8826222881864401No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA5290.8352543657435185No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA5240.8273597120030316No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA5150.813149335270155No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA5130.8099914737739603No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA5070.8005178892853759No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA4830.7626235513310385No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA4820.7610446205829412No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA4730.7468342438500647No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4600.7263081441247987No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA4540.7168345596362143No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA4280.6757823601856823No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4050.6394669529794423No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3960.6252565762465658No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA3940.622098714750371No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3720.5873622382922286No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA3250.5131524931316512No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3250.5131524931316512No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA3070.4847317396658983No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA3010.4752581551773139No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA1180.18631382827549184No Hit
GGACTACTGGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1170.18473489752739444No Hit
GGACTACTCGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1150.18157703603119968No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA1090.17210345154261533No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA1080.17052452079451796No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA1070.16894559004642057No Hit
GGACTACTAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1050.16578772855022578No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA1030.162629867054031No Hit
GGACTACTCGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1020.16105093630593362No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA1010.15947200555783625No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA990.15631414406164146No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA980.15473521331354406No Hit
GGACTACTAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC930.14684055957305714No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA920.14526162882495974No Hit
GGACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC920.14526162882495974No Hit
GGACTACTGGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC920.14526162882495974No Hit
GGACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC910.14368269807686235No Hit
GGACTACTAGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG870.1373669750844728No Hit
GGACTACTCGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG870.1373669750844728No Hit
GGACTACTGGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG860.1357880443363754No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA840.13263018284018063No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA830.13105125209208326No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA820.12947232134398584No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA770.1215776676034989No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA770.1215776676034989No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA760.11999873685540152No Hit
GGACTACTGGGGTTTCTAATCCTATTTGCTCCCCACGCTTTCGGGACTGA740.11684087535920676No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA740.11684087535920676No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA730.11526194461110935No Hit
GGACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC700.1105251523668172No Hit
GGACTACAAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG690.1089462216187198No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA680.10736729087062241No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA680.10736729087062241No Hit
GGACTACCGGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC680.10736729087062241No Hit
GGACTACCGGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG680.10736729087062241No Hit
GGACTACCGGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG680.10736729087062241No Hit
GGACTACACGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG680.10736729087062241No Hit
GGACTACCCGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG650.10263049862633027No Hit
GGACTACAAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC640.10105156787823286No Hit
GGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA640.10105156787823286No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA640.10105156787823286No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGTA2800.0295.000038
ACACGGG5750.0295.06
CGGATAT108.4461767E-4295.09
TACACGG5750.0295.05
TACCGGT108.4461767E-4295.05
GGACTAC59400.0294.503361
ACTACCG6050.0292.561983
TACTCGG9850.0292.00515
ACTCGGG9700.0291.958776
GACTACT28150.0291.332152
GACTACA14850.0291.026952
CTACTCG10050.0290.597024
ACTACTA9400.0290.292573
TACTAGG9400.0290.292575
CACGGGT5850.0289.957287
GACTACC17550.0289.957242
ACGGGTA2600.0289.326938
TACAAGG5200.0289.326935
CAAGGGT5150.0289.271857
ACAAGGG5150.0289.271856
Produced by FastQC (version 0.11.5)