FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490961

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490961
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78626
Sequences flagged as poor quality0
Sequence length301
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCTA1000.0295.02
GCCCCCT157.2147814E-6295.01
CGGCCCT500.0295.01
GTCTCGT157.2147814E-6295.0295
GCGCCTA1000.0295.02
GCGCCCT350.0295.01
CGTTCCT255.275069E-10295.01
ACGGGGC157.2147814E-6295.08
TACGCCT255.275069E-10295.01
GGGACCT303.6379788E-12295.01
GGGCCTA1050.0295.02
CCGACCT303.6379788E-12295.01
TTGCCTA500.0295.02
GGACCTA1250.0295.02
GGCGCCT303.6379788E-12295.01
CGCACCT157.2147814E-6295.01
CGATCCT303.6379788E-12295.01
ATCGCCT255.275069E-10295.01
CGCCCTA1050.0295.02
GTCGGAG157.2147814E-6295.0295
Produced by FastQC (version 0.11.5)