FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490963

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490963
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86798
Sequences flagged as poor quality0
Sequence length301
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGCCT550.0295.000031
GGTCCTA1300.0295.02
CGCGCCT157.217357E-6295.01
GCGCCTA950.0295.02
TAGCCTA1050.0295.02
GACCCTA1050.0295.02
CGTTCCT303.6379788E-12295.01
TACGCCT450.0295.01
GGAACCT255.275069E-10295.01
CGGTCCT303.6379788E-12295.01
GGCGCCT350.0295.01
CGTCCTA1750.0295.02
CGCCCTA1150.0295.02
TAGTCCT255.275069E-10295.01
CGGACCT450.0295.01
ATAGCCT206.152186E-8294.999971
GCGCCCT206.152186E-8294.999971
TTCCTCG108.451568E-4294.999973
CGCCACG108.451568E-4294.999973
TCCTACG24850.0293.21934
Produced by FastQC (version 0.11.5)