FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490965

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490965
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126293
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCCT255.293259E-10295.01
TGGTCCT600.0295.01
GGGGCCT157.225137E-6295.01
GTCCTCG108.4561214E-4294.999973
CCTACGG106950.0291.27635
CTACGGG103000.0288.985446
TACGGGA101800.0288.914557
GCCTACG21550.0288.83994
CCCTACG25250.0287.990084
ACGGGAG102350.0287.217868
CGGGAGG99300.0287.127389
ACCTACG31500.0286.103184
TCCTACG30650.0285.37524
GGCCTAC5450.0278.761473
CGGGGGC1650.0277.121229
GTCCTAC8100.0274.969123
GGTCCTA1950.0272.30772
AGGCCTA1800.0270.416662
ACGGTAG5850.0269.786328
GCCCTAC6350.0269.448823
Produced by FastQC (version 0.11.5)