EGAF00005490966 FastQC Report

Basic Statistics

Measure	Value
Filename	EGAF00005490966
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	126293
Sequences flagged as poor quality	0
Sequence length	301
%GC	46

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GGACTACTGGGGTATCTAATCTCGTATGCCGTCTTCTGCTTGCTGCTGCC	2171	1.719018472916155	Illumina PCR Primer Index 12 (96% over 28bp)
GGACTACTGGGGTTTCTAATCTCGTATGCCGTCTTCTGCTTGCTGCTGCC	2000	1.5836190446026304	RNA PCR Primer, Index 13 (96% over 28bp)
GGACTACACGGGTATCTAATCTCGTATGCCGTCTTCTGCTTGCTGCTGCC	1788	1.4157554258747516	Illumina PCR Primer Index 12 (96% over 28bp)
GGACTACACGGGTTTCTAATCTCGTATGCCGTCTTCTGCTTGCTGCTGCC	1777	1.4070455211294373	RNA PCR Primer, Index 13 (96% over 28bp)
GGACTACAAGGGTATCTAATCTCGTATGCCGTCTTCTGCTTGCTGCTGCC	1757	1.3912093306834108	Illumina PCR Primer Index 12 (96% over 28bp)
GGACTACTCGGGTATCTAATCTCGTATGCCGTCTTCTGCTTGCTGCTGCC	1677	1.3278645688993056	Illumina PCR Primer Index 12 (96% over 28bp)
GGACTACTCGGGTTTCTAATCTCGTATGCCGTCTTCTGCTTGCTGCTGCC	1628	1.2890659023065412	RNA PCR Primer, Index 13 (96% over 28bp)
GGACTACAAGGGTTTCTAATCTCGTATGCCGTCTTCTGCTTGCTGCTGCC	1621	1.283523235650432	RNA PCR Primer, Index 13 (96% over 28bp)
GGACTACCGGGGTATCTAATCTCGTATGCCGTCTTCTGCTTGCTGCTGCC	1544	1.2225539024332306	Illumina PCR Primer Index 12 (96% over 28bp)
GGACTACCGGGGTTTCTAATCTCGTATGCCGTCTTCTGCTTGCTGCTGCC	1442	1.1417893311584963	RNA PCR Primer, Index 13 (96% over 28bp)
GGACTACTAGGGTATCTAATCTCGTATGCCGTCTTCTGCTTGCTGCTGCC	1423	1.1267449502347715	Illumina PCR Primer Index 12 (96% over 28bp)
GGACTACTAGGGTTTCTAATCTCGTATGCCGTCTTCTGCTTGCTGCTGCC	1388	1.0990316169542256	RNA PCR Primer, Index 13 (96% over 28bp)
GGACTACCCGGGTATCTAATCTCGTATGCCGTCTTCTGCTTGCTGCTGCC	1352	1.0705264741513782	Illumina PCR Primer Index 12 (96% over 28bp)
GGACTACCCGGGTTTCTAATCTCGTATGCCGTCTTCTGCTTGCTGCTGCC	1262	0.9992636171442597	RNA PCR Primer, Index 13 (96% over 28bp)
GGACTACTGGGGTATCTAATCTCGTATGCCGTCTTCTGCTTGTGCTGCCT	814	0.6445329511532706	Illumina PCR Primer Index 12 (96% over 28bp)
GGACTACTGGGGTTTCTAATCTCGTATGCCGTCTTCTGCTTGTGCTGCCT	795	0.6294885702295456	RNA PCR Primer, Index 13 (96% over 28bp)
GGACTACTGGGGTATCTAATCTCGTATGCCGTCTTCTGCTTGCTGCCTCC	750	0.5938571417259864	Illumina PCR Primer Index 12 (96% over 28bp)
GGACTACTGGGGTATCTAATCTCGTATGCCGTCTTCTGCTTGGCTGCCTC	694	0.5495158084771128	Illumina PCR Primer Index 12 (96% over 28bp)
GGACTACACGGGTATCTAATCTCGTATGCCGTCTTCTGCTTGTGCTGCCT	694	0.5495158084771128	Illumina PCR Primer Index 12 (96% over 28bp)
GGACTACACGGGTTTCTAATCTCGTATGCCGTCTTCTGCTTGTGCTGCCT	688	0.5447649513433048	RNA PCR Primer, Index 13 (96% over 28bp)
GGACTACTCGGGTATCTAATCTCGTATGCCGTCTTCTGCTTGTGCTGCCT	679	0.537638665642593	Illumina PCR Primer Index 12 (96% over 28bp)
GGACTACAAGGGTATCTAATCTCGTATGCCGTCTTCTGCTTGTGCTGCCT	679	0.537638665642593	Illumina PCR Primer Index 12 (96% over 28bp)
GGACTACTGGGGTTTCTAATCTCGTATGCCGTCTTCTGCTTGGCTGCCTC	670	0.5305123799418812	RNA PCR Primer, Index 13 (96% over 28bp)
GGACTACTGGGGTTTCTAATCTCGTATGCCGTCTTCTGCTTGCTGCCTCC	667	0.5281369513749772	RNA PCR Primer, Index 13 (96% over 28bp)
GGACTACACGGGTATCTAATCTCGTATGCCGTCTTCTGCTTGCTGCCTCC	663	0.524969713285772	Illumina PCR Primer Index 12 (96% over 28bp)
GGACTACAAGGGTTTCTAATCTCGTATGCCGTCTTCTGCTTGTGCTGCCT	641	0.507549903795143	RNA PCR Primer, Index 13 (96% over 28bp)
GGACTACACGGGTTTCTAATCTCGTATGCCGTCTTCTGCTTGCTGCCTCC	612	0.4845874276484049	RNA PCR Primer, Index 13 (96% over 28bp)
GGACTACCGGGGTTTCTAATCTCGTATGCCGTCTTCTGCTTGTGCTGCCT	598	0.47350209433618645	RNA PCR Primer, Index 13 (96% over 28bp)
GGACTACAAGGGTATCTAATCTCGTATGCCGTCTTCTGCTTGCTGCCTCC	597	0.4727102848138852	Illumina PCR Primer Index 12 (96% over 28bp)
GGACTACACGGGTATCTAATCTCGTATGCCGTCTTCTGCTTGGCTGCCTC	593	0.4695430467246799	Illumina PCR Primer Index 12 (96% over 28bp)
GGACTACAAGGGTTTCTAATCTCGTATGCCGTCTTCTGCTTGCTGCCTCC	589	0.4663758086354747	RNA PCR Primer, Index 13 (96% over 28bp)
GGACTACACGGGTTTCTAATCTCGTATGCCGTCTTCTGCTTGGCTGCCTC	588	0.46558399911317333	RNA PCR Primer, Index 13 (96% over 28bp)
GGACTACTCGGGTTTCTAATCTCGTATGCCGTCTTCTGCTTGTGCTGCCT	581	0.46004133245706413	RNA PCR Primer, Index 13 (96% over 28bp)
GGACTACTAGGGTATCTAATCTCGTATGCCGTCTTCTGCTTGTGCTGCCT	571	0.452123237234051	Illumina PCR Primer Index 12 (96% over 28bp)
GGACTACCGGGGTATCTAATCTCGTATGCCGTCTTCTGCTTGTGCTGCCT	551	0.43628704678802466	Illumina PCR Primer Index 12 (96% over 28bp)
GGACTACTCGGGTATCTAATCTCGTATGCCGTCTTCTGCTTGCTGCCTCC	550	0.43549523726572337	Illumina PCR Primer Index 12 (96% over 28bp)
GGACTACTCGGGTTTCTAATCTCGTATGCCGTCTTCTGCTTGCTGCCTCC	542	0.4291607610873128	RNA PCR Primer, Index 13 (96% over 28bp)
GGACTACAGGGGTATCTAATCTCGTATGCCGTCTTCTGCTTGCTGCTGCC	535	0.42361809443120363	Illumina PCR Primer Index 12 (96% over 28bp)
GGACTACTCGGGTATCTAATCTCGTATGCCGTCTTCTGCTTGGCTGCCTC	531	0.42045085634199836	Illumina PCR Primer Index 12 (96% over 28bp)
GGACTACCCGGGTATCTAATCTCGTATGCCGTCTTCTGCTTGTGCTGCCT	531	0.42045085634199836	Illumina PCR Primer Index 12 (96% over 28bp)
GGACTACTCGGGTTTCTAATCTCGTATGCCGTCTTCTGCTTGGCTGCCTC	524	0.41490818968588916	RNA PCR Primer, Index 13 (96% over 28bp)
GGACTACAAGGGTTTCTAATCTCGTATGCCGTCTTCTGCTTGGCTGCCTC	514	0.40699009446287604	RNA PCR Primer, Index 13 (96% over 28bp)
GGACTACTAGGGTTTCTAATCTCGTATGCCGTCTTCTGCTTGTGCTGCCT	513	0.4061982849405747	RNA PCR Primer, Index 13 (96% over 28bp)
GGACTACCGGGGTTTCTAATCTCGTATGCCGTCTTCTGCTTGCTGCCTCC	509	0.4030310468513694	RNA PCR Primer, Index 13 (96% over 28bp)
GGACTACCGGGGTATCTAATCTCGTATGCCGTCTTCTGCTTGGCTGCCTC	504	0.39907199923986286	Illumina PCR Primer Index 12 (96% over 28bp)
GGACTACAAGGGTATCTAATCTCGTATGCCGTCTTCTGCTTGGCTGCCTC	501	0.39669657067295894	Illumina PCR Primer Index 12 (96% over 28bp)
GGACTACTAGGGTATCTAATCTCGTATGCCGTCTTCTGCTTGCTGCCTCC	500	0.3959047611506576	Illumina PCR Primer Index 12 (96% over 28bp)
GGACTACCGGGGTTTCTAATCTCGTATGCCGTCTTCTGCTTGGCTGCCTC	492	0.3895702849722471	RNA PCR Primer, Index 13 (96% over 28bp)
GGACTACCGGGGTATCTAATCTCGTATGCCGTCTTCTGCTTGCTGCCTCC	489	0.3871948564053431	Illumina PCR Primer Index 12 (96% over 28bp)
GGACTACCCGGGTTTCTAATCTCGTATGCCGTCTTCTGCTTGTGCTGCCT	484	0.38323580879383656	RNA PCR Primer, Index 13 (96% over 28bp)
GGACTACTAGGGTATCTAATCTCGTATGCCGTCTTCTGCTTGGCTGCCTC	470	0.3721504754816181	Illumina PCR Primer Index 12 (96% over 28bp)
GGACTACTAGGGTTTCTAATCTCGTATGCCGTCTTCTGCTTGCTGCCTCC	464	0.36739961834781026	RNA PCR Primer, Index 13 (96% over 28bp)
GGACTACAGGGGTTTCTAATCTCGTATGCCGTCTTCTGCTTGCTGCTGCC	448	0.3547306659909892	RNA PCR Primer, Index 13 (96% over 28bp)
GGACTACCCGGGTATCTAATCTCGTATGCCGTCTTCTGCTTGCTGCCTCC	441	0.34918799933488004	Illumina PCR Primer Index 12 (96% over 28bp)
GGACTACCCGGGTTTCTAATCTCGTATGCCGTCTTCTGCTTGGCTGCCTC	417	0.3301845707996484	RNA PCR Primer, Index 13 (96% over 28bp)
GGACTACCCGGGTATCTAATCTCGTATGCCGTCTTCTGCTTGGCTGCCTC	397	0.3143483803536221	Illumina PCR Primer Index 12 (96% over 28bp)
GGACTACTAGGGTTTCTAATCTCGTATGCCGTCTTCTGCTTGGCTGCCTC	396	0.3135565708313208	RNA PCR Primer, Index 13 (96% over 28bp)
GGACTACCCGGGTTTCTAATCTCGTATGCCGTCTTCTGCTTGCTGCCTCC	392	0.31038933274211555	RNA PCR Primer, Index 13 (96% over 28bp)
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA	290	0.2296247614673814	No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA	281	0.22249847576666956	No Hit
GGACTACCAGGGTATCTAATCTCGTATGCCGTCTTCTGCTTGCTGCTGCC	279	0.22091485672206695	Illumina PCR Primer Index 12 (96% over 28bp)
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA	272	0.21537219006595773	No Hit
GGACTACCAGGGTTTCTAATCTCGTATGCCGTCTTCTGCTTGCTGCTGCC	269	0.2129967614990538	RNA PCR Primer, Index 13 (96% over 28bp)
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA	268	0.21220495197675246	No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA	265	0.20982952340984853	No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA	259	0.20507866627604063	No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA	253	0.20032780914223275	No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA	247	0.19557695200842487	No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA	224	0.1773653329954946	No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA	222	0.17578171395089195	No Hit
GGACTACAGGGGTATCTAATCTCGTATGCCGTCTTCTGCTTGTGCTGCCT	218	0.1726144758616867	Illumina PCR Primer Index 12 (96% over 28bp)
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA	215	0.17023904729478276	No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA	197	0.1559864758933591	No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA	194	0.15361104732645514	No Hit
GGACTACAGGGGTTTCTAATCTCGTATGCCGTCTTCTGCTTGTGCTGCCT	185	0.1464847616257433	RNA PCR Primer, Index 13 (96% over 28bp)
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA	179	0.1417339044919354	No Hit
GGACTACAGGGGTATCTAATCTCGTATGCCGTCTTCTGCTTGGCTGCCTC	177	0.14015028544733277	Illumina PCR Primer Index 12 (96% over 28bp)
GGACTACAGGGGTTTCTAATCTCGTATGCCGTCTTCTGCTTGCTGCCTCC	161	0.12748133309051174	RNA PCR Primer, Index 13 (96% over 28bp)
GGACTACAGGGGTATCTAATCTCGTATGCCGTCTTCTGCTTGCTGCCTCC	158	0.1251059045236078	Illumina PCR Primer Index 12 (96% over 28bp)
GGACTACACGGGTTTCTAATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA	156	0.12352228547900516	Illumina Single End PCR Primer 1 (100% over 32bp)
GGACTACTGGGGTATCTAATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA	151	0.1195632378674986	Illumina Single End PCR Primer 1 (100% over 32bp)
GGACTACTGGGGTTTCTAATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA	149	0.11797961882289597	Illumina Single End PCR Primer 1 (100% over 32bp)
GGACTACACGGGTATCTAATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA	146	0.11560419025599203	Illumina Single End PCR Primer 1 (100% over 32bp)
GGACTACAGGGGTTTCTAATCTCGTATGCCGTCTTCTGCTTGGCTGCCTC	146	0.11560419025599203	RNA PCR Primer, Index 13 (96% over 28bp)
GGACTACTCGGGTTTCTAATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA	145	0.1148123807336907	Illumina Single End PCR Primer 1 (100% over 32bp)
GGACTACACGGGTTTCTAATCTCGTATGCCGTCTTCTGCCTGCTGCCTCC	145	0.1148123807336907	Illumina PCR Primer Index 12 (96% over 26bp)
GGACTACAAGGGTTTCTAATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA	145	0.1148123807336907	Illumina Single End PCR Primer 1 (100% over 32bp)
GGACTACACGGGTATCTAATCTCGTATGCCGTCTTCTGCCTGCTGCCTCC	141	0.11164514264448544	RNA PCR Primer, Index 29 (96% over 27bp)
GGACTACTGGGGTATCTAATCTCGTATGCCGTCTTCTGCCTGCTGCCTCC	141	0.11164514264448544	RNA PCR Primer, Index 29 (96% over 27bp)
GGACTACTCGGGTATCTAATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA	134	0.10610247598837623	Illumina Single End PCR Primer 1 (100% over 32bp)
GGACTACTGGGGTTTCTAATCTCGTATGCCGTCTTCTGCCTGCTGCCTCC	134	0.10610247598837623	Illumina PCR Primer Index 12 (96% over 26bp)
GGACTACTGGGGTATCTAATCTCGTATGCCGTCTTCTGCTTGGCCTCCCG	133	0.10531066646607493	Illumina PCR Primer Index 12 (96% over 28bp)
GGACTACAAGGGTATCTAATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA	131	0.10372704742147229	Illumina Single End PCR Primer 1 (100% over 32bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTGGGGG	10	8.456154E-4	294.9996	7
ACTGGGG	1555	0.0	294.05103	6
TACTGGG	1580	0.0	292.19897	5
CTGGGGT	1560	0.0	292.1631	7
GGACTAC	10935	0.0	291.8779	1
ACCGGGG	1360	0.0	291.7459	6
CGGGTTT	1925	0.0	290.40222	9
TACCCGG	1100	0.0	289.636	5
ACCCGGG	1095	0.0	289.61148	6
TACCGGG	1385	0.0	288.6097	5
ACTCGGG	1345	0.0	288.41968	6
ACAAGGG	1335	0.0	288.37042	6
CAAGGGT	1330	0.0	288.34546	7
ACACGGG	1540	0.0	288.29507	6
CGGGTAT	2005	0.0	287.643	9
CCGGGGT	1375	0.0	287.49054	7
GGGGTAT	1730	0.0	287.3262	9
GGGGTTT	1715	0.0	287.2591	9
ACGGGTT	755	0.0	287.18506	8
TCGGGTT	680	0.0	286.32315	8

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content