FastQCFastQC Report
Wed 8 May 2024
EGAF00005490969

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490969
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58096
Total Bases17.4 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATCCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1010.1738501790140457Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TAATCCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA890.15319471220049571Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACAACCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA890.15319471220049571Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTCACCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA870.1497521343982374Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCATCCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA860.14803084549710824Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTCACCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA840.14458826769484992Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACATCCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA840.14458826769484992Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATTACCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA830.14286697879372076Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACCACCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA820.14114568989259157Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCCTCCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA820.14114568989259157Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTCTCCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA810.13942440099146242Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATATCCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA810.13942440099146242Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATAACCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA810.13942440099146242Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTTCCCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA790.13598182318920407Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCTTCCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA790.13598182318920407Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACTTCCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA790.13598182318920407Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATACCCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA790.13598182318920407Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TAACCCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA790.13598182318920407Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACAGCCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA790.13598182318920407Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCATCCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA780.1342605342880749Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GTGTCCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA770.13253924538694575Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATGCCCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA770.13253924538694575Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTTACCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA770.13253924538694575Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AAATCCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA760.13081795648581657Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGGTCCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA760.13081795648581657Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTGACCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA750.1290966675846874Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGTTCCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA740.12737537868355825Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCCACCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA740.12737537868355825Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCTTCCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA730.1256540897824291Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TAAACCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA730.1256540897824291Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTAACCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA730.1256540897824291Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CAGTCCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA730.1256540897824291Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CACACCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA730.1256540897824291Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATCTCCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA720.12393280088129992Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CAAACCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA720.12393280088129992Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTCCCCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA720.12393280088129992Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATTTCCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA720.12393280088129992Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGGTCCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA720.12393280088129992Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TACTCCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA720.12393280088129992Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCTACCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA720.12393280088129992Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCAACCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA720.12393280088129992Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTTTCCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA710.12221151198017076Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTGACCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA710.12221151198017076Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTGCCCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA710.12221151198017076Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTAACCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA700.1204902230790416Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATGACCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA700.1204902230790416Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATCACCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA700.1204902230790416Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTTTCCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA700.1204902230790416Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACCTCCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA700.1204902230790416Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CACTCCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA700.1204902230790416Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCACCCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA690.11876893417791241Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGGACCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA690.11876893417791241Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGCACCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA690.11876893417791241Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TAAGCCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA690.11876893417791241Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTAGCCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA680.11704764527678325Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TACCCCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA680.11704764527678325Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGCTCCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA680.11704764527678325Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CATTCCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA680.11704764527678325Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AACACCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA680.11704764527678325Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GTTCCCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA680.11704764527678325Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTCTCCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA680.11704764527678325Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCAACCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA670.11532635637565408Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACCGCCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA670.11532635637565408Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCGTCCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA670.11532635637565408Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGTACCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA670.11532635637565408Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AATACCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA670.11532635637565408Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AAAACCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA660.11360506747452492Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATGTCCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA660.11360506747452492Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AAGTCCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA660.11360506747452492Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTGTCCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA660.11360506747452492Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTGGCCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA660.11360506747452492Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGGCCCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA660.11360506747452492Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GTGACCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA660.11360506747452492Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGTACCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA660.11360506747452492Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACGTCCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA650.11188377857339576Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GTAACCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA650.11188377857339576Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTACCCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA650.11188377857339576Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGTTCCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA640.1101624896722666Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCTCCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA640.1101624896722666Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACTACCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA640.1101624896722666Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CACGCCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA640.1101624896722666Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTTGCCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA640.1101624896722666Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCACCCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA640.1101624896722666Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GTATCCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA640.1101624896722666Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTGTCCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA640.1101624896722666Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TAGTCCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA630.10844120077113743Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCGACCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA630.10844120077113743Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CATACCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA630.10844120077113743Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCAACCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA630.10844120077113743Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCACCCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA620.10671991187000826Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CAGGCCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA620.10671991187000826Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TACACCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA620.10671991187000826Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACACCCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA620.10671991187000826Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTATCCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA610.10499862296887909Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GTCACCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA610.10499862296887909Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATGGCCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA610.10499862296887909Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCAGCCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA610.10499862296887909Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTTACCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA610.10499862296887909Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCCGCCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA610.10499862296887909Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGCTCCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA610.10499862296887909Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCCCCCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA610.10499862296887909Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTAGCCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA600.10327733406774993Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGACCCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA600.10327733406774993Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GTTTCCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA600.10327733406774993Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCCGCCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA600.10327733406774993Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATAGCCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA600.10327733406774993Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCTACCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA600.10327733406774993Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTACCCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA600.10327733406774993Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGGCCCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA600.10327733406774993Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CAAGCCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA600.10327733406774993Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CAATCCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA590.10155604516662077Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GAGACCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA590.10155604516662077Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCGTCCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA590.10155604516662077Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCCACCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA590.10155604516662077Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCCCCCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA590.10155604516662077Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATTGCCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA590.10155604516662077Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)

[FAIL]Adapter Content

Adapter graph