FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490970

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490970
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58096
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACCGTAGGATAAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT810.13942440099146242Illumina Single End PCR Primer 1 (100% over 33bp)
GGACTACCGTAGGATTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT760.13081795648581657Illumina Single End PCR Primer 1 (100% over 33bp)
GGACTACCGTAGGATGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT750.1290966675846874Illumina Single End PCR Primer 1 (100% over 33bp)
GGACTACCGTAGGAGAAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT720.12393280088129992Illumina Single End PCR Primer 1 (100% over 33bp)
GGACTACTGGGGTATCTAATCTCGTATGCCGTCTTCTGCTTGCTGCTGCC710.12221151198017076Illumina PCR Primer Index 12 (96% over 28bp)
GGACTACCGTAGGATGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT700.1204902230790416Illumina Single End PCR Primer 1 (100% over 33bp)
GGACTACCGTAGGTAATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT700.1204902230790416Illumina Single End PCR Primer 1 (100% over 33bp)
GGACTACCGTAGGTGAAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT690.11876893417791241Illumina Single End PCR Primer 1 (100% over 33bp)
GGACTACCGTAGGACCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT690.11876893417791241Illumina Single End PCR Primer 1 (100% over 33bp)
GGACTACCGTAGGAGGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT690.11876893417791241Illumina Single End PCR Primer 1 (100% over 33bp)
GGACTACCGTAGGATGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT680.11704764527678325Illumina Single End PCR Primer 1 (100% over 33bp)
GGACTACCGTAGGAAATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT670.11532635637565408Illumina Single End PCR Primer 1 (100% over 33bp)
GGACTACCGTAGGAACAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT670.11532635637565408Illumina Single End PCR Primer 1 (100% over 33bp)
GGACTACCGTAGGTTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT670.11532635637565408Illumina Single End PCR Primer 1 (100% over 33bp)
GGACTACCGTAGGGAAAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT650.11188377857339576Illumina Single End PCR Primer 1 (100% over 33bp)
GGACTACCGTAGGCTGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT650.11188377857339576Illumina Single End PCR Primer 1 (100% over 33bp)
GGACTACCGTAGGAGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT650.11188377857339576Illumina Single End PCR Primer 1 (100% over 33bp)
GGACTACCGTAGGTTGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT650.11188377857339576Illumina Single End PCR Primer 1 (100% over 33bp)
GGACTACCGTAGGATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT650.11188377857339576Illumina Single End PCR Primer 1 (100% over 33bp)
GGACTACCGTAGGAAGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT640.1101624896722666Illumina Single End PCR Primer 1 (100% over 33bp)
GGACTACCGTAGGTGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT630.10844120077113743Illumina Single End PCR Primer 1 (100% over 33bp)
GGACTACCGTAGGTGTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT630.10844120077113743Illumina Single End PCR Primer 1 (100% over 33bp)
GGACTACCGTAGGGCATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT620.10671991187000826Illumina Single End PCR Primer 1 (100% over 33bp)
GGACTACTGGGGTTTCTAATCTCGTATGCCGTCTTCTGCTTGCTGCTGCC620.10671991187000826RNA PCR Primer, Index 13 (96% over 28bp)
GGACTACCGTAGGTTTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT620.10671991187000826Illumina Single End PCR Primer 1 (100% over 33bp)
GGACTACCGTAGGTGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT610.10499862296887909Illumina Single End PCR Primer 1 (100% over 33bp)
GGACTACCGTAGGGTTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT610.10499862296887909Illumina Single End PCR Primer 1 (100% over 33bp)
GGACTACCGTAGGTCACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT610.10499862296887909Illumina Single End PCR Primer 1 (100% over 33bp)
GGACTACCGTAGGAGTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT610.10499862296887909Illumina Single End PCR Primer 1 (100% over 33bp)
GGACTACCGTAGGTTTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT610.10499862296887909Illumina Single End PCR Primer 1 (100% over 33bp)
GGACTACCGTAGGTGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT600.10327733406774993Illumina Single End PCR Primer 1 (100% over 33bp)
GGACTACCGTAGGTCAAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT600.10327733406774993Illumina Single End PCR Primer 1 (100% over 33bp)
GGACTACCGTAGGACTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT590.10155604516662077Illumina Single End PCR Primer 1 (100% over 33bp)
GGACTACCGTAGGTGATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT590.10155604516662077Illumina Single End PCR Primer 1 (100% over 33bp)
GGACTACCGTAGGTCATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT590.10155604516662077Illumina Single End PCR Primer 1 (100% over 33bp)
GGACTACAAGGGTATCTAATCTCGTATGCCGTCTTCTGCTTGCTGCTGCC590.10155604516662077Illumina PCR Primer Index 12 (96% over 28bp)
GGACTACCGTAGGTACGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT590.10155604516662077Illumina Single End PCR Primer 1 (100% over 33bp)
GGACTACCGTAGGTTGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT590.10155604516662077Illumina Single End PCR Primer 1 (100% over 33bp)
GGACTACCGTAGGAAGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT590.10155604516662077Illumina Single End PCR Primer 1 (100% over 33bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGAAT157.2050734E-6295.09
GGGGTAC108.444373E-4295.09
CTGGGGA206.134724E-8295.07
GGCTCCC950.0295.01
GGACTAC32900.0291.41341
GGACTCC3500.0286.57141
GGTGCCT1550.0285.483861
CTACCGT17550.0284.914524
CTACCGG1300.0283.653844
TACCGGG1200.0282.708345
GACTACC26650.0282.270172
ACCGTAG17000.0281.98536
TACCCGG1100.0281.590945
ACTACCG19100.0281.09953
CTCGGGT1050.0280.95247
TACCGTA17250.0278.753635
CAAGGGT900.0278.61117
CGGGTTT1700.0277.647069
TACCCGT6400.0276.56255
TCGGGTT700.0273.92868
Produced by FastQC (version 0.11.5)