FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490973

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490973
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42036
Sequences flagged as poor quality0
Sequence length301
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCCT108.436067E-4295.01
CGAACCT206.114715E-8295.01
ACGCCTA650.0295.02
CCTAGGG108.436067E-4295.05
ATGGACA206.114715E-8295.0295
GATCCTA700.0295.02
CTCCCTA206.114715E-8295.02
GGTCCTA650.0295.02
CACGCCT206.114715E-8295.01
CGCGCCT255.2204996E-10295.01
GGATCCT108.436067E-4295.01
TGAACCT255.2204996E-10295.01
CCTACGA108.436067E-4295.05
TGGCCTA650.0295.02
GCACCCT108.436067E-4295.01
CGCTCCT108.436067E-4295.01
CTACGAG108.436067E-4295.06
CTCGCCT108.436067E-4295.01
CGTACCT350.0295.01
GATGATA108.436067E-4295.0295
Produced by FastQC (version 0.11.5)