FastQCFastQC Report
Fri 10 May 2024
EGAF00005490977

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490977
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134
Total Bases40.3 kbp
Sequences flagged as poor quality0
Sequence length301
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACATCCTTCATAGAAATTTCACGCGGCGGCAAGTTGCCATACAAAACA21.4925373134328357No Hit
CAGCTTGCGGCAAAACTGCGTAACCGTCTTCTCGTTCTCTAAAAACCATT21.4925373134328357No Hit
TCAATATAACCAGTAGTGTTAACAGTCGGGAGAGGAGTGGCATTAACACC21.4925373134328357No Hit
GTCTTCTCGTTCTCTAAAAACCATTTTTCGTCCCCTTCGGGGCGGTGGTC10.7462686567164178No Hit
GCGTGAAATTTCTATGAAGGCTGTTTTCCGTTCTGGTGATTCGTCTAAGA10.7462686567164178No Hit
AATGTCAATAGATGTGGTAGAAGTCGTCATTTGGCGAGAAAGCTCAGTCT10.7462686567164178No Hit
CTTGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC10.7462686567164178No Hit
TGCCTGAGTATGGTACAGCTCATGGCCGTCTTCATTTCCATGCGGTGCAC10.7462686567164178No Hit
CGACCCTACGGGAGGCAGCGTACTACATTACAACTGACACTGAAGCACGA10.7462686567164178No Hit
TGGTTGGACTTGGTGGCAAGTCTGCCGCTGATAAAGGAAAGGATACTCGT10.7462686567164178No Hit
CCGCTTTGGCCTCTATTAAGCTCATTCAGGCTTCTGCCGTTTTGGATTTA10.7462686567164178No Hit
CGTCCAACTATCAAAATATAACGTTGACGATGTAGCTTTAGGTGTCTGTA10.7462686567164178No Hit
AGTCGGCGACTTCACGCCAGCATACGAAAGACCAGGTATATGCACAAAAT10.7462686567164178No Hit
ATAACCTACGGGAGCAGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCT10.7462686567164178No Hit
TTTGTGTGCCTGAGTATGGTACAGCTAATGGCCGTCTTCATTTCCATGCG10.7462686567164178No Hit
TTATCAGCGGCAGACTTGCCACCAAGTCCAACCAAATCAAGCAACTTATC10.7462686567164178No Hit
AAGCAACAGCAGGTTTCCGAGATTATGCGCCAAATGCTTACTCAAGCTCA10.7462686567164178No Hit
CACCCTCCCAATTGTATGTTTTCATGCCTCCAAATCTTGGAGGCTTTTTT10.7462686567164178No Hit
AATCATGCCAAGAAAAGCGGCATGGTCAATATAACCAGTAGTGTTAACAG10.7462686567164178No Hit
GGTTCCTACGGGAGGCAGCAGTCTCCCAGGACCCCCCCACACGCAGCCCA10.7462686567164178No Hit
GGCCTAACGACGTTTGGTCAGTTCCATCAACATCATAGCCAGATGCCCAG10.7462686567164178No Hit
TGCCATGCTCCGGAACAAAGCAACGCGGCACAGAATGTTTATCGGTCTGT10.7462686567164178No Hit
TTCACCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGCGAAAGCC10.7462686567164178No Hit
TCCTGAGCTTAATGCTTGGGAGCGTGCTGGTGCTGATGCTTCCTCTGCTG10.7462686567164178No Hit
GCTCCCTACGGGAGGCAGCAGATACGGCTATATCCAGAAGATCTGTTGAC10.7462686567164178No Hit
GTCAACCTCAGCACTAACCTTGCGAGTCATTTCTTTGATTTGGTCATTGG10.7462686567164178No Hit
TACCCCTACGGGAGGCAGCAGTGAGGAAAATTGGTCAATGGGCGAGAGCC10.7462686567164178No Hit
AGGTGATAAGCAGGAGAAACATACGAAGGCGCATAACGATACCACTGACC10.7462686567164178No Hit
TTGTTCCATTCTTTAGCTCCTAGACCTTTAGCAGCAAGGTCCATATCTGA10.7462686567164178No Hit
TTGTGTGCCTGAGTATGGTACAGCTAATGGCCGTCTTCATTTCCATGCGG10.7462686567164178No Hit
TCGGCGTTATAACCTCACACTCAATCTTTTATCACGAAGTCATGATTGAA10.7462686567164178No Hit
TCTCGTGCTCGTCGCTGCGTTGAGGCTTGCGTTTATGGTACGCTGGACTT10.7462686567164178No Hit
AGGTTGACTTAGTTCATCAGCAAACGCAGAATCAGCGGTATGGCTCTTCT10.7462686567164178No Hit
GCCCAGAGATTAGAGCGCATGACAAGTAAAGGACGGTTGTCAGCGTCATA10.7462686567164178No Hit
TGGTGCTATTGCTGGCGGTATTGCTTCTGCTCTTGCTGGTGGCGCCATGT10.7462686567164178No Hit
CGACTGAATGCCAGCAATCTCTTTTTGAGTCTCATTTTGCATCTCGGCAA10.7462686567164178No Hit
GCTCGCCCTGGTCGTCCGCAGCCGTTGCGAGGTACTAAAGGCAAGCGTAA10.7462686567164178No Hit
AGCTTGCAGACCCATAATGTCAATAGATGTGGTAGAAGTCGTCATTTGGC10.7462686567164178No Hit
GCCATTCAAGGCTCTAATGTTCCTAACCCTGATGAGGCCGTCCCTAGTTT10.7462686567164178No Hit
GTCAACGTTATATTTTGATAGTTTGACGGTTAATGCTGGTAATGGTGGTT10.7462686567164178No Hit
ATAAATTATGTCTAATATTCAAACTGGCGCCGAGCGTATGCCGCATGACC10.7462686567164178No Hit
TCTGGCGCTCGCCCTGGTCGTCCGCAGCCGTTGCGAGGTACTAAAGGCAA10.7462686567164178No Hit
GGTCGTCCGCAGCCGTTGCGAGGTACTAAAGGCAAGCGTAAAGGCGCTCG10.7462686567164178No Hit
AAGCTCTGATCAAGAGACAGGATGAGGATCGTTTCGCATGATTGAACAAG10.7462686567164178No Hit
GCGGCACAGACTGTTTATAGGTCTGTTGAACACGACCAGAAAACTGGCCT10.7462686567164178No Hit
TGCTTCTGGTGTGGTTGATATTTTTCATGGTATTGATAAAGCTGTTGCCG10.7462686567164178No Hit
CCGAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCTTGCTGGTCAG10.7462686567164178No Hit
ACTGCCTACGGGAGGCAGCGGTGGGAGGATCTCTTGGCAGGAGTTCAAGG10.7462686567164178No Hit
CGTGATATTGGTCGTATGGTTCTTGCTGCCGAGGGTCGCAAGGCTAATGA10.7462686567164178No Hit
CCGCGCTTCGCTTGGTCAACCCCTCAGCGGCAAAAATTAAAATTTTTACC10.7462686567164178No Hit
GATAAAAGATTGAGTGTGAGGTTATAACGCCGAAGCGGTAAAAATTTTAA10.7462686567164178No Hit
AAGGAAGCCACGATGGGAAAAGTCATGCGGCATACGCTCGGCGCCAGTTT10.7462686567164178No Hit
GACGCTTTTTCACGTTCTGGTTGGTTGTGGCCTGTTGATGCTAAAGGTGA10.7462686567164178No Hit
ATCTCTGGGCATCTGGCTATGATGTTGATGGCACTGACCAAACGTCGTTA10.7462686567164178No Hit
ATGCCGCATGACCTTTCCCATCTTGGCTTCCTTGCTGGTCAGATTGGTCG10.7462686567164178No Hit
CAGTTATATGGCTGTTGGTTTCTATGTGGCTAAATACGTTAACAAAAAGT10.7462686567164178No Hit
TGGAGGCGGTCAAAAAGCCGCCTCCGGTGGCATTCAAGGTGATGTGCTTG10.7462686567164178No Hit
CTGACGGTTATTTCCTAGACAAATTAGAGCCAATACCATCAGCTTTACCG10.7462686567164178No Hit
CCTTTGCAGTAGCGCCAATATGAGAAGAGCCATACCGCTGATTCTGCGTT10.7462686567164178No Hit
AGTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTAAGCC10.7462686567164178No Hit
CGGTAGCAAGCACATCACCTTGAATGCCACCGGAGGCGGCTTTTTGACCG10.7462686567164178No Hit
TAGCCAGATGCCCAGAGCTTAGAGCGCATGACAAGTAAAGGACGGTTGTC10.7462686567164178No Hit
GGCTGACGACCGATTAGCGGCGTTTTATGATAATCCCAATGCTTTGCGTG10.7462686567164178No Hit
ACCAGAAGGCGGTTCCTGAATGAATGGGAAGCCTTCAAGAAGGTGATAAG10.7462686567164178No Hit
TGCTTGCTACCGATAACAATACTGTAGGCATGGGTGATGCTGGTATTAAA10.7462686567164178No Hit
CAAGGTGATGTGCTTGCTACCGATAACAATACTGTAGGCATGGGTGATGC10.7462686567164178No Hit
CGGCCTGTCTCATCATGGAAGGCGCTGAATTTACGGAAAACATTATTAAT10.7462686567164178No Hit
GAAGAAGCTGGAGTAACAGAAGTGAGAACCAGCTTATCAGAAAAAAAGTT10.7462686567164178No Hit
GCTTAGGGATTTTATTGGTATCAGGGTTAATCGTGCCAAGAAAAGCGGCA10.7462686567164178No Hit
TACTACTGCTTGTTTACGAATTAAATCGAAGTGGACTGCTGGCGGAAAAT10.7462686567164178No Hit
CGATGGGCATACTGTAACCATAAGGCCACGTATTTTGCAAGCTATTTAAC10.7462686567164178No Hit
CTTACTAAAATGCAACTGGACAATCAGAAAGAGATTGCCGAGATGCAAAA10.7462686567164178No Hit
TCGCCCTGGTCGTCCGCAGCCGTTGCGAGGTACTAAAGGCAAGCGTAAAG10.7462686567164178No Hit
GGAAACCATAACGAGCATCATCTTGATTAAGCTCATTAGGGTTAGCCTCG10.7462686567164178No Hit
TGGCCCTACGGGAGGCACAGGGGTTAGGTATGACCTTGCTCCCAACCAAG10.7462686567164178No Hit
AGAAGGGTAATAAGAACGAACCATAAAAAAGCCTCCAAGATTTGGAGGCA10.7462686567164178No Hit
ACGTCCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGCGAAAGCC10.7462686567164178No Hit
GAGCTTACTAAAATGCAACTGGACAATCAGAAAGAGATTGCCGAGATGCA10.7462686567164178No Hit
ACGCTGGACTTTGTAGGATACCCTCGCTTTCCTGCTCCTGTTGAGTTTAT10.7462686567164178No Hit
CTATCCTACGGGAGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCC10.7462686567164178No Hit
TCCTTCCCCGTACGCCGGGCAATAATGTTTATGTTGGTTTCATGGTTTGG10.7462686567164178No Hit
TCGTCCTACGGAGGCAGCAGTGGGGAGCAAACAGGATTAGAAACCCTTGT10.7462686567164178No Hit
ATAAAAATGATTGGCGTATCCAACCTGCAGAGTTTTATCGCTTCCATGAC10.7462686567164178No Hit
TGATTTATGTTTGGTGCTATTGCTGGCGGTATTGCTTCTGCTCTTGCTGG10.7462686567164178No Hit
AAAGATTGAGTGTGAGGTTATAACGCCGAAGCGGTAAAAATTTTAATTTT10.7462686567164178No Hit
GCGATTTCATAGTGGAGGCCTCCAGCAATCTTGAACACTCATCCTTAATA10.7462686567164178No Hit
TGGAAGAGATTCTGTCTTTTCGTATGCAGGGCGTTGAGTTCGATAATGGT10.7462686567164178No Hit
AGAGATTCTGTCTTTTCGTATGCAGGGCGTTGAGTTCGATAATGGTGATA10.7462686567164178No Hit
TACCCCTACGGGAGGCAGCCATGGATGAGTAGATACCCTTGTAGTCCAGA10.7462686567164178No Hit
ATCGTCAACGTTATATTTTGATAGTTTGACGGTTAATGCTGGTAATGGTG10.7462686567164178No Hit
AACCAGAACGTGAAAAAGCGTCCTGCGTGTAGCGAACTGCGATGGGCATA10.7462686567164178No Hit
GCCTGTTTGGTTCGCTTTGAGTCTTCTTCGGTTCCGACTACCCTCCCGAC10.7462686567164178No Hit
AGAAATCATGAGTCAAGTTACTGAACAATCCGTACGTTTCCAGACCGCTT10.7462686567164178No Hit
GTCGAGCGTCCGGTTAAAGCCGCTGAATTGTTCGCGTTTACCTTGCGTGT10.7462686567164178No Hit
CACTATAGACCACCGCCCCGCAGGGGACGAACAATGGTTTTTAGAGAACG10.7462686567164178No Hit
ACATCCTTCATAGAAATTTCACGCGGCGGCAAGTTGCCATACAAAACAGG10.7462686567164178No Hit
ACTGGTAGCTTTAAGCGGCTCACCTTTAGCATCAACAGGCCACAACCAAC10.7462686567164178No Hit
TTACCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCC10.7462686567164178No Hit
CTAGACAAATTAGAGCCAATACCATCAGCTTTACCGTCTTTCCAGAAATT10.7462686567164178No Hit
AACGTACGGATTGTTCAGTAACTTGACTCATGATTTCTTACCTATTAGTG10.7462686567164178No Hit
CTTAAGGATATTCGCGATGAGTATAATTACCCCAAAAAGAAAGGTATTAA10.7462686567164178No Hit
AAGAGCTTCTCGAGCTGCGCAAGGATAGGTCGAATTTTCTCATTTTCCGC10.7462686567164178No Hit
ACTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC10.7462686567164178No Hit
AACACTACTGGTTATATTGACCATGCCGCTTTTCTTGGCACGATTAACCC10.7462686567164178No Hit
CAGAGTTTTATCGCTTCCATGACGCAGAAGTTAACACTTTCGGATATCTC10.7462686567164178No Hit
ATCTTGCTGCTGCATTTCCTGAGCTTAATGCTTGGGAGCGTGCTGGTGCT10.7462686567164178No Hit
AAGACCAGGTATATGCACAACATGAGATGCTTGCTTATCAACAGAAGGAG10.7462686567164178No Hit
AGCAGAACGCAAAAAGAGAGATGAGATTGAGGCTGGGAAAAGTTACTGTA10.7462686567164178No Hit
CTGACCTACGGGAGGCAGCAGGAACGGCATGTACAAAGGCTCAGAGGTGT10.7462686567164178No Hit
CAGATGCCCAGAGATTAGAGCGCATGACAAGTAAAGGACGGTTGTCAGCG10.7462686567164178No Hit
TCGCAGTAGGCGGAAAACGACCAAGCGCAAGAGTAAACATAGTGCCATGC10.7462686567164178No Hit
TCACTTAAGTGGCTGGAGACCAATAATCTCTTTAATAACCTGATTCAGCG10.7462686567164178No Hit
GTCCTTCCCCGTACGCCGGGCAATAATGTTTATGTTGGTTTCATGGTTTG10.7462686567164178No Hit
GGCCACGTATTTTGCAAGCTATTTAACTGGCGGCGATTGCGTACCCGACG10.7462686567164178No Hit
TATTGACATTATGGGTCTGCAAGCTGCTTATGCTAATTTGCATACTGACC10.7462686567164178No Hit
TATTTACCTGGCGGCGATTGCGTACCCGACGACCAAAATTAGGGTCAACG10.7462686567164178No Hit
AGAAACCAACAGCCATATAACTGGTAGCTTTAAGCGGCTCACCTTTAGCA10.7462686567164178No Hit
AACAATTTCTGGAAAGACGGTAAAGCTGATGGTATTGGCTCTAATTTGTC10.7462686567164178No Hit
CGTCCCTACGGGAGGCAAGCAGAAGAAGGCATACGAAATACACCAGTAGT10.7462686567164178Illumina Single End Adapter 1 (95% over 21bp)
CTAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC10.7462686567164178No Hit
AAAATTACGTGCAGAAGGAGTGATGTAATGTCTAAAGGTAAAAAACGTTC10.7462686567164178No Hit
CAGCAATCTTGAACACTCATCCTTAATACCTTTCTTTTTGGGGTAATTAT10.7462686567164178No Hit
ACCTGTAGGAAGTGTCCGCATAAAGTGCACCGCATGGAAATGAAGACGGC10.7462686567164178No Hit
TCAGGCTTCTGCCGTTTTGGCTTTAACCGAAGATGATTTCGATTTTCTGA10.7462686567164178No Hit
CCCGGCGTACGGGGAAGGACGTCAATAGTCACACAGTCCTTGACGGTATA10.7462686567164178No Hit
TTTTAGTAAGCTCTTTTTGATTCTCAAATCCGGCGTCAACCATACCAGCA10.7462686567164178No Hit
ATACGCCAATCATTTTTATCGAAGCGCGCATAAATTTGAGCAGATTTGTC10.7462686567164178No Hit
GAGCAGATTTGTCGTCACAGGTTGCGCCGCCAAAACGTCGGCTACAGTAA10.7462686567164178No Hit
AATTTTAATTTTTGCCGCTGAGGGGTTGACCAAGCGAAGCGCGGTAGGTT10.7462686567164178No Hit
ATTCTGTGCCGCGTTTCTTTGTTCCTGAGCATGGCACTATGTTTACTCTT10.7462686567164178No Hit
TCTGTTGGTGCTGATATTGCTTTTGATGCCGACCCTAAATTTTTTGCCTG10.7462686567164178No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)