FastQCFastQC Report
Fri 10 May 2024
EGAF00005490978

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490978
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134
Total Bases40.3 kbp
Sequences flagged as poor quality0
Sequence length301
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA21.4925373134328357No Hit
TGTCTACAGTAGAGTCAATAGCAAGGCCACGACGCAATGGAGAAAGACGG10.7462686567164178No Hit
TTACTTGAGGATAAATTATGTCTAATATTCACACTGGCGCCGAGCGTATG10.7462686567164178No Hit
GTTATAGATATTCAAATAACCCTGAAACAAATGCTTAGGGATTTTATTGG10.7462686567164178No Hit
TCAAGAAGTCCTTTACCAGCTTTAGCCATAGCACCAGAAACAAAACTAGG10.7462686567164178No Hit
CGAATCACGAGGGAGCTAAAAAGTGTTTTTGCACTCTGGATTAACGCAGT10.7462686567164178No Hit
TGAGAAGAGCCATACCGCTGATTCTGCGTTTGCTGATGAACTAAGTCAAC10.7462686567164178No Hit
CTTCTCCTCGGGCATCCAAGCTCTCTGGCGACCTAGACTACCGACACATC10.7462686567164178No Hit
GCAGGGGCTTCGGCCCCTTACTTGAGGCTAAATTATGTCTAATATTCAAA10.7462686567164178No Hit
TGGTTTCTATGTGGCTAAATACGTTAACAAAAAGTCAGATATGGACCTTG10.7462686567164178No Hit
GGTCTACCAGGCTAGCGACCATCCGTGTACCCGGTTGGCTTCCCTCCCGC10.7462686567164178No Hit
AGGTTTCCGAGATTATGCGCCACATGCTTACTCAAGCTCAAACGGCTGGT10.7462686567164178No Hit
AGCATCAACAGGCCACAACCAACCAGAACGTGAAAAAGCGTCCTGCGTGT10.7462686567164178No Hit
CCCTCCCGACTGCCTATGATGTTTATCCTTTGGATGGTCGCCATGATGGT10.7462686567164178No Hit
ATAGAAATTTCACGCGGCGGCAAGTTGCCATACAAAACCGGGTCGCCAGC10.7462686567164178No Hit
GAATGTCACGCTGATTATTTTGACTTTGAGCGTATCGAGGCTCTTAAACC10.7462686567164178No Hit
CGCTGAATAGCAAAGCCTCTACGCGATTTCATAGTGGAGGCCTCCAGCAA10.7462686567164178No Hit
AGCGGCAAAAATTAAAATTTTTACCGCTTCGGCGTTATAACCTCACACTC10.7462686567164178No Hit
CGCCGTGGATGCCTGACCGTACCGAGGCTAACCCTAATGAGCTTAATCAA10.7462686567164178No Hit
CCATGATGTTATTTCTTCATTTGGAGGTAAAACCTCTTATGACGCTGACA10.7462686567164178No Hit
TTGAATTATGGCGAGAAATAAAAGTCTGAAACATGATTAACCTCCTAAGC10.7462686567164178No Hit
AAATTATGTCTAATATTCAAACTGGCGCCGAGCGTATGCCGCATGCCCTT10.7462686567164178No Hit
CGACTGCCTATGATGTTTATCCTTTGGATGGTCGCCATGATGGTGGTTAT10.7462686567164178No Hit
TATGAGAAGAGCCATACCGCTGATTCTGCGTTTGCTGATGAACTAAGTCA10.7462686567164178No Hit
CGCTGACAACCGTCCTTTACTTGTCATGCGCTCTAATCTCTGGGCATCTG10.7462686567164178No Hit
CGAGGTTTTACCTCCAAATGAAGAAATAACATCATGGTAACGCCGCATGA10.7462686567164178No Hit
GTTTGAGCTTGAGTAAGCATTTGGCGCATAATCTCGGAAACCTGCTGTTG10.7462686567164178No Hit
CACCCATGCCTACAGTATTGTTATCGGTAGCACGCACATCACCTTGAATG10.7462686567164178No Hit
ACGGCGTCTACTGCTCGCGTTGCGTCTATTATGGAAAACACCAATCTTTC10.7462686567164178No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCA10.7462686567164178No Hit
AGCCTCGATACGCTCAAAGTCAAAATAATCAGCGTGACATTCAGAAGGGT10.7462686567164178No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA10.7462686567164178No Hit
CCGGTTAAAGCCGCTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGG10.7462686567164178No Hit
TGATGCTGGTATTAAATCTGCCATTCAAGGCTCTAATGTTCCTAACCCTG10.7462686567164178No Hit
AAAAAATTTAGGGTCGGCATCAAAAGCAATATCAGCACCAACAGAAACAA10.7462686567164178No Hit
TTTGATTCTCAAATCCGGCGTCAACCATACCAGCAGAGGAAGCATCAGCA10.7462686567164178No Hit
CGACTACCCGGGTCTCTACCTCGGTACGACCTCAGCCCGGCGCGCCCGCC10.7462686567164178No Hit
TGTTGCAGTGGAATAGTCAGGTTAAATTTAATGTGACCGTTTATCGCAAT10.7462686567164178No Hit
GCATCAGGTTCTTAAAGAGATTATTTGTCTCCAGCCACTTAAGTGAGGTG10.7462686567164178No Hit
TGCAACAACTGCCCGGACTGGAAACACTGGTCATAATCCTGGTGGCGAAT10.7462686567164178No Hit
GCGCCGTGGATGCCTGACCGTACCGAGGCTAACCCTAATGAGCTTCATCA10.7462686567164178No Hit
GCTTTATCAATACCATGAAAAATATCACCCACACCAGAAGCAGCATCAGT10.7462686567164178No Hit
AGTGAGTTGTTCCATTCTTTAGCTCCTCGACCTTTAGCAGCAAGGTCCAT10.7462686567164178No Hit
CCTTTAGCAGCAAGGTCCATATCTGACTTTTTGTTAACGTATTTAGCCAC10.7462686567164178No Hit
GCCTCATCAGGGTTAGGAACATTAGAGCCTTGAATGGCAGATTTAATACC10.7462686567164178No Hit
TGCTAATTTGCATACTGACCAAGAACGTGATTACTTCATGCAGCGTTACC10.7462686567164178No Hit
TGGATACGCCAATCATTTTTATCGAAGCGCGCATAAATTTGAGCAGATTT10.7462686567164178No Hit
GCTTTGCTATTCAGCGTTTGATGAATGCAATGCGACAGGCTCATGCTGAT10.7462686567164178No Hit
TGAGTAAGCATTTGGCGCATAATCTCGGAAACCTGCTGTTGCTTGGAAAG10.7462686567164178No Hit
CCGTGTTTCCTGCGCGTACACGCAAGGTAAACGCGAACAATTCAGCGGCT10.7462686567164178No Hit
GGACGACCAGGGCGAGCGCCAGAACGTTTTTTACCTTTAGACATTACATC10.7462686567164178No Hit
CGTGGAGGCCTCCAGCAATCTTGCACACTCATCCTTAATACCTTTCTTTT10.7462686567164178No Hit
GCTTTAAAATAGTTGTTATAGATATTCAAATAACCCTGCAACAAATGCTT10.7462686567164178No Hit
ATTAAGTTCATGAAGGATGGTGTTAATGCCACTCCTCTCCCGACTGTTCA10.7462686567164178No Hit
TTTCTCATGAGTCGAAAAATTATCTTGACAAAGCAGGAATTACTACTGCT10.7462686567164178No Hit
AGACCTATAAACATTCTGTGCCGCGTTTCTTTGTTCCTGAGCATGGCACT10.7462686567164178No Hit
ACTACTCCGGTTATCGCTGGCGACTCCTTCGCGATGGACGCCGTTGGCGC10.7462686567164178No Hit
CAAAGACGAGCGCCTTTACGCTTGCCTTTAGTACCTCGCAACGGCTGCGG10.7462686567164178No Hit
AGTATGGTTCTTGCTGCCGAGGGTCGCAAGGCTCATGATTCACACGCCGA10.7462686567164178No Hit
TTCCATTCTTTAGCTCCTAGACCTTTCGCAGCAAGGTCCATATCTGACTT10.7462686567164178No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA10.7462686567164178No Hit
GATGATGCTCGTTATGGTTTCCGTTGCTGCCATCTCAAAAACATTTGGAC10.7462686567164178No Hit
GGACTACTGGGGTATCTCGTATGCCGTCTTCTGCTTGCCTTCCGTAGGGA10.7462686567164178TruSeq Adapter, Index 25 (96% over 27bp)
GAAAACATACAATTGGGAGGGTGTCAATCCTGACGGTTATTTCCTAGACA10.7462686567164178No Hit
TTTTCTGCTTAGGAGTTTAATCATGTTTCAGCCTTTTATTTCTCGCCATA10.7462686567164178No Hit
GCTGATGGTTGGTTTATCGTTTTTGACACTCTCACGTTGGCTGACGACCG10.7462686567164178No Hit
GGACATAAAAAGTAAAAATGTCTACAGTAGAGTCAATAGCAAGGCCACGA10.7462686567164178No Hit
TCCCCAATGCTTGGCTTCCATAAGCAGATGGATAACCGCATCAAGCTCTT10.7462686567164178No Hit
GAAGTTTAAGATTGCTGAGGGTCAGTGGTATCGTTATGCGCCTTCGTATG10.7462686567164178No Hit
CGCACATCACCTTGAATGCCACCGGAGGCGGCTTTTTGACCGCCTCCAAA10.7462686567164178No Hit
CAGCAGCAAGATAATCACGAGTATCCTTTCCTTTATCAGCGGCAGACTTG10.7462686567164178No Hit
ACCAATAAAATCCCTACGCATTTGTTTCAGGGTTATTTGAATATCTATAA10.7462686567164178No Hit
AAGGTCCATATCTGACTTTTTGTTAACGTATTTCGCCACATCGAACCCAA10.7462686567164178No Hit
TGCTTAATCCAACTTACCAAGCTGGGTTACGACGCGACGCCGTTCAACCA10.7462686567164178No Hit
CTTTCCAAGCAACAGCAGGTTTCCGAGCTTATGCGCCAAATGCTTACTCA10.7462686567164178No Hit
GGACTACAAGGGTATCTACTCATCCATGGCTGCCTCCCGTAGGGGTAAGA10.7462686567164178No Hit
TAACAATACTGTAGGCCTGGGTGATGCTGGTCTTAAATCTGCCATTCAAG10.7462686567164178No Hit
CCGGAGCAAGGTGAGATGACAGGAGATCCTGCCCCGGCACTTCGCCCAAT10.7462686567164178No Hit
AGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACTGCTGCCTCCGTAG10.7462686567164178No Hit
GCTCTTAAACCTGCTATTGAGGCTTGTGGCATTTCTACTCTTTCTCAATC10.7462686567164178No Hit
TAGAGGCGTTTTATGATAATCCCAATGCTTTGCGTGACTATTTTCGTGAT10.7462686567164178No Hit
ACCGGAGTAGTTGAAATGGTAATAAGACGACCAATCTGACCAGCAAGGAA10.7462686567164178No Hit
AGTAACTTTTCCCAGCCTCAATCTCATCTCTCTTTTTGCGTTCTGCTTCA10.7462686567164178No Hit
AAGTTGGGGGAGCACATTGTAGCATTGTGCCAATTCATCCATTAACTTCT10.7462686567164178No Hit
GACCAAGAACGTGATTACTTCATGCAGCGTTACCATGATGTTATTTCTTC10.7462686567164178No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA10.7462686567164178No Hit
AGTACGAACGTCAGTGTTTCCTGCGCGTACACGCAAGGTACACGCGAACA10.7462686567164178No Hit
TCTCTTCCAAGAGCTTGATGCGGTTATCCATCTGCTTATGGAAGCCAAGC10.7462686567164178No Hit
CCTCTCGGCAATCTCTTTCTGATTGTCCAGTTGCATTTTAGTAAGCTCTT10.7462686567164178No Hit
ATACTCAGGCACACAAAAATACTGATAGCAGTCGGCGTGTGAATCATTAG10.7462686567164178No Hit
GCCAGGCTCATGCTGATGGTTGGTTTATCGTTTTTGACACTCTCACGTTG10.7462686567164178No Hit
GAGCCTTGAATGGCAGATTTAATACCAGCATCACCCATGCCTACAGTATT10.7462686567164178No Hit
GGACTACACGGGTATCTAATTTTAATTCTCCCAGGATTTCAAATTAACTG10.7462686567164178No Hit
AGTCAAAGCACCTTTAGCGTTAAGGTACTGACTCTCTTTAGTCGCAGTAG10.7462686567164178No Hit
GGACTACTAGGGTTTCTAATCCCGTTTGCTACCCTAGCTTTCGCGCTATG10.7462686567164178No Hit
CGCATAATCTCGGAAACCTGCTGTTGCTTGGAAAGATTGGTGTTTTCCAT10.7462686567164178No Hit
TTCTGGTGATTTGCAAGAACGCGTACTTATTCGCCACCATGATTATGACC10.7462686567164178No Hit
CAAAAACATTTGGACTGCTCCGCTTCCTCCTGAGACTGAGCTTTCTCGCC10.7462686567164178No Hit
CAACCATACCAGCAGAGGAAGCATCAGCACCCGCACGCTCCCAAGCATTA10.7462686567164178No Hit
GGACTACTAGGGTATCTAATCCCGTTTGCTACCCTAGCTTTCGCGCTATG10.7462686567164178No Hit
ACGACCGTGACCGGCGTGCCCGATTTCTGCTCCCTGCGTCCTACACTGCG10.7462686567164178No Hit
ATTTAGTAGCGGTAAAGTTAGACCAAACCATGAAACCAACATAAACATTA10.7462686567164178No Hit
TTGCTGGCGACCCTGTTTTGTATGGCAACTTGCCGCCGCGTGAAATTTCT10.7462686567164178No Hit
AGCATCGGACTCAGATAGTAATCCACGCTCTTTTAAAATGTCAACAAGAG10.7462686567164178No Hit
AATCATCTTCGGTTAAATCCAAAACGGCAGACGCCTGAATGAGCTTAATA10.7462686567164178No Hit
TGTCCCTCATCGTCACGTTTATGGTGCACAGTGGATTAAGTTAATGAAGG10.7462686567164178No Hit
TCAACATACATATCACCATTATCGAACTCAACGCCCTGCATACGAAAAGA10.7462686567164178No Hit
ACAAATAATCTCTTTAATAACCTGATTCAGCGAAACCAATCCGCGGCATT10.7462686567164178No Hit
TTGACTATTAGACATAATTTATCCTCAAGTAAGGGGCCGAAGCCCCTGCA10.7462686567164178No Hit
GTCTTTTCGTATGCAGGGCGTTGAGTTCGATAATGGTGATATGTATGTTG10.7462686567164178No Hit
TGATGAATGCACTGCGACAGGCTCATGCTGATGGTTGGTTTATCGTTTTT10.7462686567164178No Hit
GTCAGTGTTTCCTGCGCGTACACGCAAGGTACACGCGAACAATTCAGCGG10.7462686567164178No Hit
CATGAACTTAATCCACTGTTCACCATAAACGTGACGATGAGGGACATAAA10.7462686567164178No Hit
TTATGCGGACACTTCCTACAGGTAGCGTTGACCCTAATTTTGGTCGTCGG10.7462686567164178No Hit
TTAGGGTCGGCATCAAAAGCAATATCAGCACCAACAGAAACAACCTGATT10.7462686567164178No Hit
CGACGTTTTGTCGTCACAGGTTGCGCCGCCAAAACGTCGGCTACAGTAAC10.7462686567164178No Hit
AAAATATCAACCACACCAGAAGCAGCATCAGTGACGACATTAGAAATATC10.7462686567164178No Hit
TGCAGCAGCAAGATAATCACGAGTATCCTTTCCTTTATCAGCGGCAGACT10.7462686567164178No Hit
AATAGTCACGCAAAGCATTGGGCTTATCATAAACCGCCTCTAATCGGTCG10.7462686567164178No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCA10.7462686567164178No Hit
ATCCTTCATGAACTTAATCCACTGTTCACCATAAACGTGACGATGAGGGA10.7462686567164178No Hit
ACTATTTTAAAGCGCCGTGGATGCCTGACCGTACCGAGGCTAACCCTAAT10.7462686567164178No Hit
CCTGAAACAAATGCTTAGGGATTTTATTGGTCTCAGGGTTCATCGTGCCA10.7462686567164178No Hit
CGGATTTGAGAATCAAAAAGAGCTTACTAAAATGCAACTGGACAATCAGA10.7462686567164178No Hit
TTCGTTCTTATTACCCTTCTGACTGTCACGCTGATTATTTTGACTTTGAG10.7462686567164178No Hit
AAGCATCCCCCAGGCCCTACCATCATCCTCCCCAATCCACACCGCCGGGG10.7462686567164178No Hit
GACTGCTCCACTTCCTCCTGAGACTGAGCTTTCTCGCCAAATGACGACTT10.7462686567164178No Hit
ACAGGCAAGCGTAAAGGCGCTCGTCTTTGGTATGTAGGTGGTCAACAATT10.7462686567164178No Hit
CAGGTATTACGGATGAGTGTTCAAGATTGCTGGCGGCCTCCACTATGAAA10.7462686567164178No Hit
TGGTTGTGGCCTGTTGATGCTAAAGGTGAGCCGCTTAAAGCTACCAGTTA10.7462686567164178No Hit
TTCTTACCTATTAGTGGTTGAACAGCATCGGACTCAGATAGTAATCCACG10.7462686567164178No Hit
CCACGACGCAATGGAGAAAGACGGAGAGCGCCAACGGCGTCCATCTCGAA10.7462686567164178No Hit
AGAGCTTGCCGCGATGCAAAATGAGACTCAAAAAGAGATTGCTGGCATTC10.7462686567164178No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)