FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005490979

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490979
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146
Sequences flagged as poor quality0
Sequence length301
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAAGGATACTCGTGATTATCTTGCTGCTGCATTTCCTGAGCTTAATGCT21.36986301369863No Hit
CTTTGCGTGACTATTTTCGTGATATTGGTCGTATGGTTCTTGCTGCCGAG10.684931506849315No Hit
CAGAACGTGAAAAAGCGTCCTGCGTGTAGCGAACTGCGATGGGCATACTG10.684931506849315No Hit
CAGACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCC10.684931506849315No Hit
AGCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC10.684931506849315No Hit
CCATCAACTAACGATTCTGTCAAAAACTGACGCGTTGGATGAGGAGAAGT10.684931506849315No Hit
GGGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC10.684931506849315No Hit
GGTGATAAGCAGGAGAAACATACGAAGGCGCATAACGATACCACTGACCC10.684931506849315No Hit
GCTTAATAGAGGCCAAAGCGGTCTGGAAACGTACGGATTGTTCAGTAACT10.684931506849315No Hit
TTCCCAGCCTCAATCTCATCTCTCTTTTTGCGTTCTGCTTCAATATCTGG10.684931506849315No Hit
TAACTGGTAGCTTTAAGCGGCTCACCTTTAGCATCAACAGGCCACAACCA10.684931506849315No Hit
AGGGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC10.684931506849315No Hit
TAGTCGCAGTAGGCGGAAAACGAACAAGCGCAAGAGTAAACATAGTGCCA10.684931506849315No Hit
GGAGTGATGTACTGTCTAAAGGTAAAAAACGTTCTGGCGCTCGCCCTGGT10.684931506849315No Hit
ATTCACACGCCGACTGCTATCAGTATTTTTGTGTGCCTGAGTATGGTACA10.684931506849315No Hit
ACATAAACATTATTGCCCGGCGTACGGGGAAGGACGTCAATAGTCACACA10.684931506849315No Hit
TAATGTCTAAAGGTAAAAAACGTTCTGGCGCTCGCCCTGGTCGTCCGCAG10.684931506849315No Hit
ATTTCCTACGGATCTCGTATGCCGTCTTCTGCTTGAAAAGAAAAAAGGGC10.684931506849315RNA PCR Primer, Index 30 (96% over 28bp)
GATGCTGCTTCTGGTGTGGTTGATATTTTTCATGGTATTGATAAAGCTGT10.684931506849315No Hit
GTTTCTCCTGCTTATCACCTTCTTGAAGGCTTCCCATTCATTCAGGAACC10.684931506849315No Hit
AATGCTTAGGGATTTTATTGGTATCAGGGTTAATCGTGCCAAGAAAAGCG10.684931506849315No Hit
CTCCTACGGGCAGCAGTGGGGAATTTTGGACAACGGGGGCAACCCTGATC10.684931506849315No Hit
AAATACTTTAACAAAAAGTCGGATATGGACCTTGCTGCTAAAGGTCTAGG10.684931506849315No Hit
ATTTGGTCATTGGTCAAATACTGACCAGCCGTTTGAGCTTGAGTAAGCAT10.684931506849315No Hit
CCTACAGTATTGTTATCGGTAGCAAGCACATCACCTTGAATGCCACCGGA10.684931506849315No Hit
GGATTGACACCCTCCCACTTGTATGTTTTCATGCCTCCAAATCTTGGAGG10.684931506849315No Hit
ATCAGGTTGTTTCTGTTGGTGCTGATATTGCTTTTGATGCCGACCCTAAA10.684931506849315No Hit
CACACCAGAAGCAGCATCAGTGACGACATTAGAAATATCCTTTGCAGTAG10.684931506849315No Hit
GAACACGACCAGAACACTGGCCTAACGACGTTTGGTCAGTTCCATCAACA10.684931506849315No Hit
AGCGAACCAAACAGGCAAAAAATTTAGGGTCGGCATCAAAAGCAATATCA10.684931506849315No Hit
CAACTGGACAATCAGAAAGAGATTGCCGAGATGCAAAATGAGACTCAAAA10.684931506849315No Hit
TCTCCCTGCTGCTGTGGATCAGCGGAGCCTACGGAGCCATCCAGATGACC10.684931506849315No Hit
TAGGTCTGTTGAACACGACCAGAAAACTGGCCTAACGACGTTTGGTCAGT10.684931506849315No Hit
AGTTGTTATAGATATTCAAATAACCCTGAAACAAATGCTTAGGGATTTTA10.684931506849315No Hit
CAGGACGCTTTTTCACGTTCTGGTTGGTTGTGGCCTGTTGATGCTAAAGG10.684931506849315No Hit
ATGTTTATAGGTCTGTTGAACACGACCAGAAAACTGGCCTAACGACGTTT10.684931506849315No Hit
ATAAAAGTCTGAAACATGATTAAACTCCTAAGCAGAAAACCTACCGCGCT10.684931506849315No Hit
CAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC10.684931506849315No Hit
GCTCGCCCTGGTCGTCCGCAGCCGTTGCGAGGTACTAAAGGCAAGCGTAA10.684931506849315No Hit
CAAACCTACGGGAGGCAGCAGTGAGGAATATTGGGCAACGGAGGGAACTC10.684931506849315No Hit
GCTCAAATTTCTGCGCGCTTCGATAAAAATGATTGGCGTATCCAACCTGC10.684931506849315No Hit
TCATGAAGGATGGTGTTCATGCCACTCCTCTCCCGACTGTTAACACTACT10.684931506849315No Hit
GCTTAATATGCTTGGCACGTTCGTCAAGGACTGGTTTAGATATGAGTCAC10.684931506849315No Hit
TGTTCCATTCTTTAGCTCCTAGACCTTTAGCAGCAAGGTCCATATCTGAC10.684931506849315No Hit
ACATTAGAGCCTTGAATGGCAGATTTAATACCAGCATCACCCATGCCTAC10.684931506849315No Hit
CGAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC10.684931506849315No Hit
TCAAACGGCCTGTCTCATCATGGAAGGCGCTGAATTTACGGAAAACATTA10.684931506849315No Hit
ATAAACCAACCATCAGCATGAGCCTGTCGCATTGCATTCATCAAACGCTG10.684931506849315No Hit
ATTATACTCATCGCGAATATCCTTAAGAGGGCGTTCAGCAGCCAGCTTGC10.684931506849315No Hit
TAGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC10.684931506849315No Hit
TGAAACAAATGCTTAGGGATTTTATTGGTATCAGGGTTAATCGTGCCAAG10.684931506849315No Hit
TGCTGCTGCATTTCCTGAGCTTAATGCTTGGGAGCGTGCTGGTGCTGATG10.684931506849315No Hit
GATATTCGCGATGAGTATAATTACCCCAAAAAGAAAGGTATTAAGGATGA10.684931506849315No Hit
CAAGCTGGGTTACGACGCGACGCCGTTCAACCAGATATTGAAGCAGAACG10.684931506849315No Hit
TGAGTATAATTACCCCAAAAAGAAAGGTATTAAGGATGAGTGTTCAAGAT10.684931506849315No Hit
CGCGCAGGAAACACTGACGTTCTTACTGACGCAGAAGAAAACGTGCGTCA10.684931506849315No Hit
GGCTAAATACGTTAACAAAAAGTCAGATATGGACCTTGCTGCTAAAGGTC10.684931506849315No Hit
CCCGCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTC10.684931506849315No Hit
AGATAATCCCAATGCTTTGCGTGACTATTTTCGTGATATTGGTCGTATGG10.684931506849315No Hit
ATATCCTACGGGAGGCGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA10.684931506849315TruSeq Adapter, Index 18 (96% over 28bp)
CAATAGATGTGGTAGAAGTCGTCATTTGGCGAGAAAGCTCAGTCTCAGGA10.684931506849315No Hit
GACGCTTTTTCACGTTCTGGTTGGTTGTGGCCTGTTGATGCTAAAGGTGA10.684931506849315No Hit
ACTGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC10.684931506849315No Hit
CCATCTTGGCTTCCTTGCTGGTCAGATTGGTCGTCTTATTACCATTTCAA10.684931506849315No Hit
AGCCTTATGGCCGTCAACATCCATATCACCATTATCGAACTCAACGCCCT10.684931506849315No Hit
CAAGTTACTGAACAATCCGTACGTTTCCAGACCGCTTTGGCCTCTATTAA10.684931506849315No Hit
GTGTAGCGAACTGCGATGGGCATACTGTAACCATAAGGCCACGTATTTTG10.684931506849315No Hit
GCTGAATTTACGGAAAACATTATTAATGGCGTCGAGCGTCCGGTTAAAGC10.684931506849315No Hit
CACTTTATGCGGACACTTCCTACAGGTAGCGTTGACCCTAATTTTGGTCG10.684931506849315No Hit
CTGTTGAACACGACCAGAAAACTGGCCTAACGACGTTTGGTCAGTTCCAT10.684931506849315No Hit
GCTCCCTACGGGAGGCAGCAGTAGGGAATATTCGGCAATGGACGAAAGCC10.684931506849315No Hit
GCGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGTCGGCAGAC10.684931506849315No Hit
TAAACATTATTGCCCGGCGTACGGGGAAGGACGTCAATAGTCACACAGTC10.684931506849315No Hit
GCAAGAACGCGTACTTATTCGCCACCATGATTATGACCAGTGTTTCCAGT10.684931506849315No Hit
GCGCTTCGCTTGGTCAACCCCTCAGCGGCAACAATTAAAATTTTTACCGC10.684931506849315No Hit
TTTTACCTTTAGACATTACATCACTCCTTCTGCACGTAATTTTTGACGCA10.684931506849315No Hit
TAACCATAAGGCCACGTATTTTGCAAGCTATTTAACTGGCGGCGATTGCG10.684931506849315No Hit
AACATTATTAATGGCGTCGAGCGTCCGGTTAAAGCCGCTGAATTGTTCGC10.684931506849315No Hit
GCTTCGATAAAAATGATTGGCGTATCCAACCTGCAGAGTTTTATCGCTTC10.684931506849315No Hit
TGAATGCCAGCAATCTCTTTTTGAGTCTCATTTTGCATCTCGGCAATCTC10.684931506849315No Hit
GAGCTCTCTGGCTAACTAGAGAACCCACTGCTTACTGGCTTATCGAAATT10.684931506849315No Hit
TTTGTCATTGTGAGCATTTTCATCCCGAAGTTGCGGCTCATTCTGATTCT10.684931506849315No Hit
CTCATTCAGGCTTCTGCCGTTTTGGATTTAACCGAAGATGATTTCGATTT10.684931506849315No Hit
CTACCCTACGGGAGGCAGCAGTGAGGAATATTGGGCAATGGAGGCAACTC10.684931506849315No Hit
TATTCTGGCGTGAAGTCGCCGACTGAATGCCAGCAATCTCTTTTTGAGTC10.684931506849315No Hit
GCAGTAGGCGGAAAACGAACAAGCGCAAGAGTAAACATAGTGCCATGCTC10.684931506849315No Hit
GCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCTTGCTGGTCAGATTG10.684931506849315No Hit
GGAAGGCGCTGAATTTACGGAAAACATTATTCATGGCGTCGAGCGTCCGG10.684931506849315No Hit
TTATCTTGCTGCTGCATTTCCTGAGCTTAATGCTTGGGAGCGTGCTGGTG10.684931506849315No Hit
GAAGCAGAACGCAAAAAGAGAGATGAGATTGAGGCTGGGAAAAGTTACTG10.684931506849315No Hit
GGGATGCAAATGCTCACAATGACAAATCTGTCCACGGAGTGCTTAATCCA10.684931506849315No Hit
GTGGCCTGTTGATGCTAAAGGTGAGCCGCTTAAAGCTACCAGTTATATGG10.684931506849315No Hit
TTTTCATCCCGAAGTTGCGGCTCATTCTGATTCTGAACAGCTTCTTGGGA10.684931506849315No Hit
AGAAGGAGTGATGTAATGTCTAAAGGTAAAAAACGTTCTGGCGCTCGCCC10.684931506849315No Hit
CCGTTTGAGCTTGAGTAAGCATTTGGCGCATAATCTCGGAAACCTGCTGT10.684931506849315No Hit
ACCAAGCTGGGTTACGACGCGACGCCGTTCAACCAGATATTGAAGCAGAA10.684931506849315No Hit
CCCTCAGCGGCAAAAATTAAAATTTTTACCGCTTCGGCGTTATAACCTCA10.684931506849315No Hit
GCAATAGCACCAAACATAAATCACCTCACTTAAGTGGCTGGAGACAAATA10.684931506849315No Hit
CCTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC10.684931506849315No Hit
TTCTGCCGTTTCTGATAAGTTGCTTGATTTGGTTGGACTTGGTGGCAAGT10.684931506849315No Hit
TGTCTAGGACATAACCGTCAGGATTGACACCCTCCCAATTGTATGTTTTC10.684931506849315No Hit
ATTACCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGACGAAAGTC10.684931506849315No Hit
GTAAAGTTAGCCCAAACCATGAAACCAACATAAACATTATTGCCCGGCGT10.684931506849315No Hit
GACCGATTAGAGGCGTTTTATGATAATCCCAATGCTTTGCGTGACTATTT10.684931506849315No Hit
TTAAGATTGCTGAGGGTCAGTGGTATCGTTATGCGCCTTCGTATGTTTCT10.684931506849315No Hit
CTGCGCAAGGCTAGGTCGAATTTTCTCATTTTCCGCCAGCAGTCCACTTC10.684931506849315No Hit
AAAGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC10.684931506849315No Hit
GGACGCTTTTTCACGTTCTGGTTGGTTGTGGCCTGTTGATGCTAAAGGTG10.684931506849315No Hit
ATCAGCGTGACATTCAGCAGAGTAATAAGAACGAACCATAAAAAAGCCTC10.684931506849315No Hit
GTCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC10.684931506849315No Hit
AAGCAGAACGCAAAAAGAGAGATGAGATTGAGGCTGGGAAAAGTTACTGT10.684931506849315No Hit
AATGTGCTCCCCCAACTTGATATTAATAACACTATAGACCACCGCCCCGA10.684931506849315No Hit
GTCAGCCAACGTGAGAGTGTCAAAAACGATAAACCAACCATCAGCATGAG10.684931506849315No Hit
GCGGCGATTGCGTACCCGACGACCAAAATTAGGGTCAACGCTACCTGTAG10.684931506849315No Hit
ACTATGTTTACTCTTGCGCTTGTTCGTTTTCCGCCTACTGCGACTAAAGA10.684931506849315No Hit
TGTCTAATATTCAAACTGGCGCCGAGCGTATGCCGCATGACCTTTCCAAT10.684931506849315No Hit
CATCTCAAAAACATTTGGACTGCTCCGCTTCCTCCTGAGACTGAGCTTTC10.684931506849315No Hit
CAGTAGGCGGAAAACGAACAAGCGCAAGAGTAAACATAGTGCCATGCTCA10.684931506849315No Hit
AGTTTTGTTTCTGGTGCTATGGCTAAAGCTGGTAAAGGACTTCTTGAAGG10.684931506849315No Hit
GATGGCGAAAGGTCGCAAAGTAAGAGCTTCTCGAGCTGCGCAAGGATAGG10.684931506849315No Hit
TTGCCATACAAAACAGGGTCGCCAGCAATATCGGTATAAGTCAAAGCACC10.684931506849315No Hit
TCAATCCTGACGGTTATTTCCTAGACAAATTAGAGCCAATACCATCAGCT10.684931506849315No Hit
TTCCATCAACATCATAGCCAGATGCCCAGAGATTAGAGCGCATGACAAGT10.684931506849315No Hit
ATAATAACCACCATCATGGCGACCATCCAAAGGATAAACATCATAGGCAG10.684931506849315No Hit
AATATTCAAACTGGCGCCGAGCGTATGCCGCATGACCTTTCCCATCTTGG10.684931506849315No Hit
TAATCCTACGGGAGGCAGCAATGGGGAATATTGTACAATGGGCGCAAGCC10.684931506849315No Hit
GAGCATGGCACTATGTTTACTCTTGCGCTTGTTCGTTTTCCGCCTACTGC10.684931506849315No Hit
TTAAGCTCATTAGGGTTAGCCTCGGTACGGTCAGGCATCCACGGCGCTTT10.684931506849315No Hit
AGTACGCCGGGCAATAATGTTTATGTTGGTTTCATGGTTTGGTCTAACTT10.684931506849315No Hit
ATGATGGTGGTTATTATACCGTCAAGGACTGTGTGACTATTGACGTCCTT10.684931506849315No Hit
ACGCTCGGCGCCAGTTTGAATATTAGACATAATTTATCCTCAAGTAAGGG10.684931506849315No Hit
TTTTGCCAGCTATTTAACTGGCGGCGATTGCGTACCCGACGACCAAAATT10.684931506849315No Hit
CTTTAGGTGTCTGTAAAACAGGTGCCGAAGAAGCTGGAGTAACAGAAGTG10.684931506849315No Hit
CCGTCAAGGACTGTGTGACTATTGACGTCCTTCCCCGTACGCCGGGCAAT10.684931506849315No Hit
AATCTCTTTAGTCGCAGTAGGCGGAAAACGAACAAGCGCAAGAGTAAACA10.684931506849315No Hit
AGCCATACCGCTGATTCTGCGTTTGCTGATGAACTAAGTCAACCTCAGCA10.684931506849315No Hit
TATGACGCTGACAACCGTCCTTTACTTGTCATGCGCTCTAATCTCTGGGC10.684931506849315No Hit
GTACCCTACGGGAGGCAGCAGTGGGGGATATTGCACAATGGAGGAAACTC10.684931506849315No Hit
GAACAGCTTCTTGGGAAGTAGCGACAGCTTGGTTTTTAGTGAGTTGTTCC10.684931506849315No Hit
GCTGATCAAGGAAAGGATACTCGTGATTATCTTGCTGCTGCATTTCCTGA10.684931506849315No Hit
AACCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGCCGAGAGGC10.684931506849315No Hit
GATATGAGTCACATTTTGTTCATGGTAGAGATTCTCTTGTTGACATTTTA10.684931506849315No Hit
AGCGTTACCATGATGTTATTTCTTCATTTGGAGGTAAAACCTCTTATGAC10.684931506849315No Hit
CATGACAAGTAAAGGACGGTTGTCAGCGTCATAAGAGGTTTTACCTCCAA10.684931506849315No Hit
GAGATTATGCGCCCAATGCTTACTCAAGCTCAAACGGCTGGTCAGTATTT10.684931506849315No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)