FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490980

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490980
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146
Sequences flagged as poor quality0
Sequence length301
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACCAGGGTTTCTAATCCTGTTTGATCCCCATGCTTTCGAGCCTCA21.36986301369863No Hit
AAATGTCAACAAGAGAATCTCTACCATGAACAAAATGTGACTCATATCTA10.684931506849315No Hit
TGCTCAGGAACAAAGAAACGCGGCACAGAATGTTTATAGGTCTGTTGAAC10.684931506849315No Hit
GCCGCGTGAAATTTCTATGAAGGATGTTTTCCGTTCTGGTGATTCGTCTA10.684931506849315No Hit
GGAAACACTGGTCATAATCATGGTGGCGAATAAGTACGCGTTCTTGCAAA10.684931506849315No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA10.684931506849315No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA10.684931506849315No Hit
CTCTTTTAACATGTCAACAAGAGAATCTCTACCATGAACAAAATGTGACT10.684931506849315No Hit
TGAACATAATAAGCAATGACGGCAGCAATAACCTCAACCGGCGCAGGAAA10.684931506849315No Hit
CTGGCGAAAGGTCGCAAAGTAAGAGCTTCTCGAGCTGCGCAAGGATAGGT10.684931506849315No Hit
AATGCTTTGCGTGACTATTTTCGTGATATTGGTCGTATGGTTCTTGCTGC10.684931506849315No Hit
CAAAGTAAGAGCTTCTCGAGCTGCGCAAGGATAGGTCGAATTTTCTCATT10.684931506849315No Hit
ACATTTGGACTGCTCCGCTTCCTCCTGAGACTGAGCTTTCTCGCCAAATG10.684931506849315No Hit
TAGACATTTTTACTTTTTATGTCCCTCATCGTCACGTTTATGGTGAACAG10.684931506849315No Hit
AGCTTGAGTCAGCATTTGGCGCATAATCTCGGAAACCTGCTGTTGCTTGG10.684931506849315No Hit
TACGCCAATCATTTTTATCGAAGCGCGCATAAATTTGAGCAGATTTGTCG10.684931506849315No Hit
ATTCAGCGTTTGATGAATGCAATGCGACAGGCTCATGCTGATGGTTGGTT10.684931506849315No Hit
AACTTGCCGCCGCGTGAAATTTCTATGAAGGATGTTTTCCGTTCTGGTGA10.684931506849315No Hit
GCGGCAAGTTGCCATACAAAACAGGGTCGCCAGCAATATCGGTATAAGTC10.684931506849315No Hit
CGTTTTTGACACTCTCACGTTGGCTGACGACCGATTAGAGGCGTTTTATG10.684931506849315No Hit
AAGTGTCCGCATAAAGTGCACCGCATGGAAATGAAGACGGCCATTAGCTG10.684931506849315No Hit
TTTTTGCGTTCTGCTTCAATATCTGGTTGAACGGCGTCGCGTCGTAACCC10.684931506849315No Hit
ATAAAAAGTCAACATGTCTACAGTAGAGTCAATAGCAAGGCCACGACGCA10.684931506849315No Hit
GTTCTGGTTGGTTGTGGCCTGTTGATGCTAAAGGTGAGCCGCTTAAAGCT10.684931506849315No Hit
ACTTCCTACAGGTAGCGTTGACCCTACTTTTGGTCGTCGGGTACGCAATC10.684931506849315No Hit
TGGTGTTTTCCATAATCGACGCAACGCGAGCAGTAGACTCCTTCTGTTGA10.684931506849315No Hit
AATCCAACTTACCAAGCTGGGTTACGCCGCGACGCCGTTCAACCAGATAT10.684931506849315No Hit
GCACGAGAGCGGTCAGTAGCAATCCAAACTTTGTTACTCGTCAGAAAATC10.684931506849315No Hit
ACTGCCTATGATGTTTATCCTTTGGATGGTCGCCATGATGGTGGTTATTA10.684931506849315No Hit
CATGCGGCATACGCTCGGCGCCAGTTTGAATATTAGACATCATTTATCCT10.684931506849315No Hit
GAAGGAGTCGCCAGCGATAACCGGAGTAGTTGAAATGGTAATAAGACGAC10.684931506849315No Hit
AGTTGTTATAGATATTCAAATAACCCTGAAACAAATGCTTAGGGATTTTA10.684931506849315No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCGAGTCACGCTTTCGCGACGCA10.684931506849315No Hit
ACTAGGGACGGCCTCATCAGGGTTAGGCACATTAGAGCCTTGAATGGCAG10.684931506849315No Hit
ATCTTGGCTTCCTTGCTGGTCAGATTGGTCGTCTTATTACCATTTCAACT10.684931506849315No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCCGA10.684931506849315No Hit
AGCGACGAGCACGAGAGCGGTCAGTAGCAATCCAAACTTTGTTACTCGTC10.684931506849315No Hit
TGAGCAGATTTGTCGTCACAGGTTGCGCCGCCACAACGTCGGCTACAGTA10.684931506849315No Hit
CCGTCCCTAGTTTTGTTTCTGGTGCTATGGCTAAAGCTGGTAAAGGACTT10.684931506849315No Hit
CCGATTTGGCGGCATGAAAACATACAATTGGGAGGGTGTCAATCCTGACG10.684931506849315No Hit
GCCGATACTTGGAACAATTTCTGGAAAGACGGTAAAGCTGCTGGTATTGG10.684931506849315No Hit
ACCTTTAGCATCAACAGGCCACAACCCCCCAGACCGTGAAAAAGCGTCCT10.684931506849315No Hit
ATCATGGTAACGCTGCATGAAGTAATCCCGTTCTTGGTCAGTATGCAAAT10.684931506849315No Hit
TTAACAGTCGGGAGAGGAGTGGCATTAACACCATCCTTCATGAACTTACT10.684931506849315No Hit
AAAAAGCCTCCAAGATTTGGAGGCATGAAAACATACAATTGGGAGGGTGT10.684931506849315No Hit
ACGAGATTGCCGAGATGCAAAATGAGCCTCACAAAGAGATTGCTGGCATT10.684931506849315No Hit
GGTCAACGCTACCTGTAGGAAGTGTCCGCATAAAGTGCACCGCATGGAAA10.684931506849315No Hit
GGGCTCAGGAGTTATCTCATCCGGTCTGTACCCCTCGCTTATCTGCTCCT10.684931506849315No Hit
CCCTGGCCGAAGGTCCAGGGGTAGTTGTAGTCCTGCAGGCAGTAGTAGGT10.684931506849315No Hit
TGACCAGCAAGGAAGCCAAGATGGGAAAGGTCATGCGGCATACGCTCGGC10.684931506849315No Hit
GAAATGACTCGCAAGGTTAGTGCTGAGGTTGACTTAGTTCATCAGCAACC10.684931506849315No Hit
CAAGAACGCGTACTTATTCGCCACCATGATTCTGACCAGTGTTTCCAGTC10.684931506849315No Hit
TGCGGCATACGCTCGGCGCCAGTTTGAATATTAGACATAATTTATCCTCA10.684931506849315No Hit
CTTCGGCACCTGTTTTACAGACACCTACAGCTACATCGTCAACGTTATAT10.684931506849315No Hit
TACTAAAGGCCAGCGTAAAGGCGCTCGTCTTTGGTATGTAGGTGGTCAAC10.684931506849315No Hit
ATCTGGTTGAACGGCGTCGCGTCGTAACCCAGCTTGGTAAGTTGGATTAA10.684931506849315No Hit
ACCTTCTTGAAGGCTTCCCATTCATTCAGGACCCGCCTTCTGGTGATTTG10.684931506849315No Hit
CACGGCGCTTTAAAATAGTTGTTATAGATATTCCAATAACCCTGAAACAA10.684931506849315No Hit
ATGAGTTTGTATCTGTTACTGAGAAGTTAATGGATGAATTGGCACAATGC10.684931506849315No Hit
CGTTTTGGATTTAACCGAAGATGATTTCGATTTTCTGACGAGTAACAAAG10.684931506849315No Hit
AAAAGTCTGAAACATGATTAAACTCCTAAGCAGAAAACCTACCGCGCTTC10.684931506849315No Hit
TATTCCACTGCAACAACTGAACGGACTGGAACCACTGGTCATAATCATGG10.684931506849315No Hit
CTCATTCTGATTCTGAACAGCTTCTTGGGAAGTAGCGACAGCTTGGTTTT10.684931506849315No Hit
CATGACCTTTCCCATCTTGGCTTCCTTGCTGGTCAGATTGGTCGTCTTAT10.684931506849315No Hit
GAAAGGTATTCAGGCTGAGTGTTCAAGATTGCTGGAGGCCTCCACTATGA10.684931506849315No Hit
CCATTTGGACTGCTCCGCTTCCTCCTGAGACTGAGCTTTCTCGCCAAATG10.684931506849315No Hit
AAATAGTTGTTATAGATATTCAAATAACCCTGAAACAAATGCTTAGGGAT10.684931506849315No Hit
GGACTACTCGCATATTTAAGCCTGTTTGATCCCCACGCTTGCGCACATCA10.684931506849315No Hit
GGACTACCCGGGTTTCTAATCCAGCAGGCGACCCAATACGAGAAGCCTAG10.684931506849315No Hit
CTGTGTGACTATTGACGTCCTTCCCCGTACGCCGGGCAATAATGTTTATG10.684931506849315No Hit
GGCGACTTCACGCCAGAATACGAAAGCCCAGGTATATGCACAAAATGAGA10.684931506849315No Hit
ACTGCTATCAGTATTTTTGTGTGCCTGAGTATGGTACAGCTAATGGCCGT10.684931506849315No Hit
ACATTTTAATTTTTGCCGCTGAGGGGTTGACCAAGCGAAGCGCGGTAGGT10.684931506849315No Hit
TGGTTTCCGTTGCTGCCATCTCAAAACCATTTGGACTGCTCCGCTTCCTC10.684931506849315No Hit
GAACATAATACGCAATGACGGCAGCAATAAACTCAACAGGAGCAGGAAAG10.684931506849315No Hit
AGAAACCAACAGCCATATAACTGGTAGCTTTAAGCGGCTCCCCTTTAGCA10.684931506849315No Hit
GAATATCTATAACAACTATTTTCAAGCGCCGTGGATGCCTGACCGTACCG10.684931506849315No Hit
ACTTCGATTTAATTCGTAACCAAGCAGTAGTAATTCCTGCTTTATCAAGA10.684931506849315No Hit
TGTGCCAATTCATCCATTAACTTCTCAGTAACAGATACAAACTCATCACG10.684931506849315No Hit
GGACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA10.684931506849315No Hit
CTTAATCAAGATGATGCTCGTTCTGGTTTCCGTTGCTGCCCTCTCAAACA10.684931506849315No Hit
CAAGAGCAGAAGCAATACCGCCCGCAATAGCACCAAACATAAATCACCTC10.684931506849315No Hit
ATACTGACCAAGAACGTGATTACTTCATGCAGCGTTACCATGATGTTATT10.684931506849315No Hit
GGACTACTCGGGTTTCTAATCCTGTTTAATCCCCATGCTTTCGAGCCTCA10.684931506849315No Hit
TATTTGAATATCTATAACAACTATTTTCAAGCGCCGTGGATGCCTGACCG10.684931506849315No Hit
CGACTACTAGGGTTTCTAATCCTGTTTGATCCCCATGCTTTCGAGCCTCA10.684931506849315No Hit
ACCAAGAACGTGATTACTTCATGCAGCGTTACCATGATGTTATTTCTTCA10.684931506849315No Hit
CCTCAGCAATCTTAAACTTCTTCGACGCATCACCAGAACGGAAAACATCC10.684931506849315No Hit
AAAGATTGGTGTTTTCCATAATAGACGCAACGCGAGCAGTAGACTCCTTC10.684931506849315No Hit
GGCTACAGTAACTTTTCCCAGCCTCAATCTCCTCTCTCTTTTTGCGTTCT10.684931506849315No Hit
ATTTGGACTGCTCCGCTTCCTCCTGAGACTGAGCTTTCTCGCCAAATGAC10.684931506849315No Hit
AATTCAAACTTTTTTTCTGATAAGCTGGTTCTCACTTCTGTTACTCCAGC10.684931506849315No Hit
GCCGAGCGTCTGCCGCATGACCTTTCCCATCTTGGCTTCCTTGCTGGTCA10.684931506849315No Hit
GACATTAGAAATATCCTTTGCAGTAGCGCCAATCTGAGAAGAGCCATACC10.684931506849315No Hit
CTGCTGTTGCTTGGAAAGATTGGTGTTTTCCATAATAGACGCAACGCGAG10.684931506849315No Hit
TCCTACAGGTAGCGTTGACCCTAATTTTGGTCGTCGGGTACGCAATCGCC10.684931506849315No Hit
GCTATTCAGCGTTTGATGAATGCAATGCGACAGGCTCATGCTGATGGTTG10.684931506849315No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA10.684931506849315No Hit
AGATATTCAAATAACCCTGAAACAAATGCTTAGGGATTTTATTGGTATCA10.684931506849315No Hit
CCACTACCCGCTCACCTTGTTCTCTCCCCCCTCCACTCGGTCACACCTGC10.684931506849315No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA10.684931506849315No Hit
TAGGGACGGCCTCATCCGGGTTAGGAACATTCGAGCCTTGAATGGCAGCT10.684931506849315No Hit
GCTGCTGCATTTCCTGAGCTTAATGCTTGGGAGCGTGCTGGTGCTGATGC10.684931506849315No Hit
GGCCTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGTCTCA10.684931506849315No Hit
TTAATGCTTGGGCGCGTGCTGGTGCTGATGCTTCCTCTGCTGGTATGGTT10.684931506849315No Hit
ATGCTCGTTATGGTTTCCGTTGCTGCCATCTCAAAAACATTTGGACTGCT10.684931506849315No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA10.684931506849315No Hit
CCCTTACCACGCTGGGTTACGACGCGACGCCGTTCAACCAGATATTGAAG10.684931506849315No Hit
TCGCGTTGCGTCTATTATGGAAAACACCAATCTTTCCAAGCAACAGCAGG10.684931506849315No Hit
CAACAGGCCACAACCAACCAGAACGTGAAAACGCGTCCTGCGTGTAGCGA10.684931506849315No Hit
AATACTGACCAGCCGTTTGAGCTTGAGTAAGCATTTGGCGCATAATCTCG10.684931506849315No Hit
GCAGATTTGTCGTCACAGGTTGCGCCGCCAAAACGTCGGCTACAGTAACT10.684931506849315No Hit
CTGCGTCATGGCAGCGATAAAACTCTGCAGGTTGGATACGCCAATCATTT10.684931506849315No Hit
GGACTACACGCGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCCCCTCA10.684931506849315No Hit
TTCTGGTGCTATGGCTAAAGCTGGTACAGGACTTCTTGAAGGTACGTTGC10.684931506849315No Hit
TATCGAGGCTCTTAAACCTGCTATTGAGGCTTGTGGCATTTCTACTCTTT10.684931506849315No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCACCACGCTTTCGCACCTGA10.684931506849315No Hit
GGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCACGCTTTCGCGCCTCA10.684931506849315No Hit
ATTATCATAAAACGCCTCTAATCGGTCGTCAGCCAACGTGAGAGTGTCAA10.684931506849315No Hit
TTTACGAATTAAATCGAAGTGGCCTGCTGGCGGAAAATGAGAAAATTCGA10.684931506849315No Hit
TGAGCTTGAGTAAGCATTTGGCGCATAATCTCGGAAACCTGCTGTTGCTT10.684931506849315No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA10.684931506849315No Hit
TCTGCACGTAATTTTTGACGCACGTTTTCTTCTGCGTCAGTAAGAACGTC10.684931506849315No Hit
CGGCGCCAGTTTGAATATTAGACATAATTTATCCTCAAGTAAGGGGCCGA10.684931506849315No Hit
GAGTATGGTACAGCTAATGGCCGTCTTCATTTCCATGCGGTGCACTTTAT10.684931506849315No Hit
GACATAATTTATCCTCAAGTAAGGGGCCGAAGCCCCTGCAATTAAAATTG10.684931506849315No Hit
AGTTGATGGCGCAAGGTCGCAAAGTACGAGCTTCTCGAGCTGCGCAAGGA10.684931506849315No Hit
GGTAATGGTGGTTTTCTTCATTGCATTCAGATGGATACATCTGTCAACGC10.684931506849315No Hit
GGACTACTGGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGTGCCTCA10.684931506849315No Hit
TTTCGTATGCAGGGCGTTGAGTTCGATAATGGTGATATGTCTGTTGACGG10.684931506849315No Hit
GGCGCCGAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCTTGCTGG10.684931506849315No Hit
TCCTCCTGAGACTGAGCTTTCTCGCCAAATGACGACTTCTACCACATCTA10.684931506849315No Hit
ACGGCCTCATCAGGGTTAGGAACATTAGAGCCTTGAATGGCAGATTTAAT10.684931506849315No Hit
TGTAGTAGGCGGTGATGCCGCTCATGGTGATGGCGGACACCCACTCCAGT10.684931506849315No Hit
CCAGCTTCTTCGGCACCTGTTTTACAGACACCTCAAGCTACATCGTCAAC10.684931506849315No Hit
GTGCCGCGTTTCTTTGTTCCTGAGCATGGCACTATGTTTACTCTTGCGCT10.684931506849315No Hit
AGCAAGGTCCATATCTGACTTTTTGTTAACGTATTTAGCCACATAGACAC10.684931506849315No Hit
GCGAGCAGTAGACTCCTTCTGTTGATAAGCAAGCATCTCATTTTGTGCAT10.684931506849315No Hit
TCGTTTTTGACACTCTCACGTTGGCTGACGACCGATTAGAGGCGTTTTAT10.684931506849315No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA10.684931506849315No Hit
AGCATTGTGCCAATTCATCCATTAACTTCTCAGTAACAGATACAAACTCA10.684931506849315No Hit
CGATGAGGGACATAAAAAGTAACACTGTCTACAGTAGAGTCAATAGCAAG10.684931506849315No Hit
TTAATCATGTTTCAGACTTTTCTTTCTCGCCATCATTCAAACTTTTTTTC10.684931506849315No Hit
AATTATGGCGAGAAATAAAAGTCTGAAACATGATTAAACTCCTAAGCAGA10.684931506849315No Hit
TATAACGTTGACGATGTAGCTTTAGGTGTCTGTCAAACAGGTGCCGAAGA10.684931506849315No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTTTT150.002244395259.000004290-294
Produced by FastQC (version 0.11.5)