FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490985

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490985
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79589
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCCT350.0295.000031
CGAGCCT350.0295.000031
CGTCCTA950.0295.000032
CGATCCT350.0295.000031
TCGCCTA750.0295.02
GCCCCTA1150.0295.02
GTCGCCT206.149094E-8295.01
GTACCCT400.0295.01
CCGGGAG108.4502506E-4295.06
ACGCCTA850.0295.02
CCGGCCT303.6379788E-12295.01
GATCCTA1650.0295.02
TTGTCCT600.0295.01
CGCGCCT108.4502506E-4295.01
GCGCCTA750.0295.02
CGCCTAG108.4502506E-4295.03
AAGCCTA900.0295.02
TACGCCT255.275069E-10295.01
CTCGGGA255.275069E-10295.06
CTGCCTA400.0295.02
Produced by FastQC (version 0.11.5)