FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490987

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490987
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74143
Sequences flagged as poor quality0
Sequence length301
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCCT206.146183E-8295.01
TCGCCTA1150.0295.02
GCGACCT255.2568794E-10295.01
GCGCGAA550.0295.0295
TGAACCT550.0295.01
TACGCCT255.2568794E-10295.01
CTGCCTA900.0295.02
CTCGCCT400.0295.01
CGTACCT350.0295.01
GTCCCTA500.0295.02
TGGGCCT206.146183E-8295.01
GATTCCT400.0295.01
CCGCCCT350.0295.01
CGCCCTA800.0295.02
GTGCCTA400.0295.02
AAGTCCT650.0295.01
TACGGCG157.213115E-6294.999977
CGTTCCT157.213115E-6294.999971
TGCGCCT157.213115E-6294.999971
ACGGCGG157.213115E-6294.999978
Produced by FastQC (version 0.11.5)