FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490989

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490989
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132241
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCTAG108.45657E-4295.03
CGCGCCT206.164373E-8295.01
TGACGCC108.45657E-4295.0295
GTAGCCT500.0295.01
CGGGCCT255.293259E-10295.01
GGAACCT400.0295.01
CCGACCT500.0295.01
CGGTCCT650.0295.01
GGCGCCT206.164373E-8295.01
TTCGCCT650.0295.01
CGCCCTA1400.0295.02
GCCTACG25000.0290.284
CCCTACG28000.0290.258944
CCTACGG122000.0289.801245
TACGGGA118550.0288.903447
CTACGGG119600.0288.710276
ACCTACG35550.0288.361484
ACGGGAG119100.0287.569278
TCCTACG34350.0287.270754
CCCCTAC6900.0286.449283
Produced by FastQC (version 0.11.5)