FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490992

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490992
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77105
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA36924.788275727903509No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA27983.6288178457946954No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA21802.8273134038000127No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA21262.757279035082031No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA17282.2410997989754233No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA16172.097140263277349No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA15031.9492899293171648No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA14851.9259451397445042No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA13971.8118150573892746No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA13031.6899033785098245No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA11501.4914726671422085No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA11301.4655340120614746No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA11171.4486738862589974No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA11161.4473769535049608No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA10991.4253290966863368No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA10111.311199014331107No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA9081.1776149406653265No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA8171.0595940600479865No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA7951.031061539459179No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA7761.0064198171324816No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA6670.8650541469424811No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA6460.8378185591077102No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA6340.82225536605927No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA6210.8053952402567927No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA6040.7833473834381688No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA5870.7612995266195448No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA5790.7509240645872511No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA5700.7392516698009208No Hit
GGACTACAAGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG5420.7029375526878932No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA5040.6536541080344984No Hit
GGACTACTAGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG4540.5888074703326632No Hit
GGACTACAAGGGTTTCTAAGCCTATTTGCTCCCCACGCTTTCGTGACTGA4420.5732442772842229No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4420.5732442772842229No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA4370.5667596135140394No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA4340.5628688152519292No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4330.5615718824978925No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA4200.5447117566954154No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA3770.4889436482718371No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3630.4707865897153232No Hit
GGACTACTAGGGTTTCTAAGCCTATTTGCTCCCCACGCTTTCGTGACTGA3620.46948965696128653No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3610.46819272420724983No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA3500.45392646391284613No Hit
GGACTACACGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG3380.4383632708644057No Hit
GGACTACAAGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG3370.437066338110369No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA3370.437066338110369No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA3230.4189092795538551No Hit
GGACTACTGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG3110.4033460865054147No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA2850.3696258349004604No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA2790.3618442383762402No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA2760.3579534401141301No Hit
GGACTACTCGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG2710.3514687763439466No Hit
GGACTACACGGGTTTCTAAGCCTATTTGCTCCCCACGCTTTCGTGACTGA2650.34368717981972635No Hit
GGACTACTAGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG2630.34109331431165296No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2460.319045457493029No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2380.30866999546073537No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2310.29959146618247845No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2310.29959146618247845No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2290.296997600674405No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2250.2918098696582582No Hit
GGACTACCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG2210.28662213864211145No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2150.2788405421178912No Hit
GGACTACTGGGGTTTCTAAGCCTATTTGCTCCCCACGCTTTCGTGACTGA2100.2723558783477077No Hit
GGACTACTCGGGTTTCTAAGCCTATTTGCTCCCCACGCTTTCGTGACTGA2090.27105894559367094No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA2030.2632773490694507No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1990.25808961805330394No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1970.25549575254523055No Hit
GGACTACTGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG1950.25290188703715716No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA1940.2516049542831204No Hit
GGACTACAAGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1930.2503080215290837No Hit
GGACTACACGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG1900.2464172232669736No Hit
GGACTACCCGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG1870.2425264250048635No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1850.23993255949679007No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA1730.22436936644834965No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1720.22307243369431295No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1680.21788470267816612No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1640.2126969716620193No Hit
GGACTACTCGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG1640.2126969716620193No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA1590.20621230789183578No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1560.20232150962972573No Hit
GGACTACCCGGGTTTCTAAGCCTATTTGCTCCCCACGCTTTCGTGACTGA1560.20232150962972573No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA1530.1984307113676156No Hit
GGACTACTAGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1510.1958368458595422No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1510.1958368458595422No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1480.19194604759743208No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1480.19194604759743208No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1400.18157058556513844No Hit
GGACTACCGGGGTTTCTAAGCCTATTTGCTCCCCACGCTTTCGTGACTGA1390.18027365281110175No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1380.17897672005706505No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1370.17767978730302833No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1330.17249205628688152No Hit
GGACTACCCGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG1320.17119512353284483No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGTCTCA1310.16989819077880813No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA1300.1686012580247714No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1260.1634135270086246No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA1240.1608196615005512No Hit
GGACTACAAGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1200.15563193048440438No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1190.15433499773036768No Hit
GGACTACAAGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1190.15433499773036768No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1180.153038064976331No Hit
GGACTACTGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1150.14914726671422088No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGTCTCA1150.14914726671422088No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1130.14655340120614746No Hit
GGACTACAAGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1130.14655340120614746No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCA1110.14395953569807407No Hit
GGACTACCGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG1070.13877180468192724No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1060.13747487192789054No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA1050.13617793917385385No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1030.13358407366578043No Hit
GGACTACAAGGGTTTCTAATCCTGTTCGCTACCCACGCTCTCGCGCATCA1010.130990208157707No Hit
GGACTACTAGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1010.130990208157707No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1010.130990208157707No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA970.1258024771415602No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA960.12450554438752351No Hit
GGACTACACGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG950.1232086116334868No Hit
GGACTACTAGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG940.12191167887945009No Hit
GGACTACAAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC910.11802088061733998No Hit
GGACTACCAGGGTTTCTAAGCCTATTTGCTCCCCACGCTTTCGTGACTGA900.1167239478633033No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA900.1167239478633033No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCA890.11542701510926659No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA890.11542701510926659No Hit
GGACTACAAGGGTTTCTAATCCTGTTCTATCCCCACGCTTTCGTGCCTCA890.11542701510926659No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCA880.1141300823552299No Hit
GGACTACTAGGGTTTCTAATCCTGTTCGCTACCCACGCTCTCGCGCATCA870.11283314960119319No Hit
GGACTACTCGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG870.11283314960119319No Hit
GGACTACTAGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA850.11023928409311977No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGTCTCA840.10894235133908306No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGTCTCA840.10894235133908306No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA830.10764541858504637No Hit
GGACTACTAGGGTTTCTAATCCTGTTCTATCCCCACGCTTTCGTGCCTCA790.10245768756889956No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA780.10116075481486286No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA780.10116075481486286No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA780.10116075481486286No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA780.10116075481486286No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGGTA950.0295.08
AAGGTAT500.0295.08
ACTAGGT2200.0295.06
GGGGTAT1500.0295.09
TGGGGTA650.0295.08
ACTCGGG7800.0295.06
TACCAGG3050.0294.999975
CGTTAGA108.4497465E-4294.99997295
CGGGGTA400.0294.999978
ACAGGGT400.0294.999976
CTAGTGT108.4497465E-4294.999977
TGTTAGA157.2142484E-6294.99997295
AAGGGTA1450.0294.999978
CTGGGTA157.2142484E-6294.999977
TAGTGTT108.4497465E-4294.999978
GGTTAGA108.4497465E-4294.99997295
AGTGTTT108.4497465E-4294.999979
GACTAGT108.4497465E-4294.999972
GGACTAC74400.0294.801761
GACTACT31350.0294.059022
Produced by FastQC (version 0.11.5)