FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490995

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490995
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78565
Sequences flagged as poor quality0
Sequence length301
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC810.10309934449182205No Hit
TCTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC810.10309934449182205No Hit
TTCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC800.10182651307834277No Hit
CTGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC800.10182651307834277No Hit
TTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC800.10182651307834277No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCCTA1550.0295.000032
TGATCCT255.275069E-10295.01
GCCCCTA1150.0295.02
TTGGCCT350.0295.01
ATCTCCT500.0295.01
GTCGCCT157.214763E-6295.01
AGTCCCT350.0295.01
TGCCCCT303.6379788E-12295.01
AGATCCT303.6379788E-12295.01
CGAACCT157.214763E-6295.01
TCACCCT350.0295.01
CCTAGGG255.275069E-10295.05
GAGTCCT550.0295.01
TGGACCT303.6379788E-12295.01
ACACCCT303.6379788E-12295.01
GATCCTA1300.0295.02
CGACCTA1100.0295.02
AAGACCT303.6379788E-12295.01
CACGCCT157.214763E-6295.01
GCGCCTA700.0295.02
Produced by FastQC (version 0.11.5)