FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491001

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491001
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63091
Sequences flagged as poor quality0
Sequence length301
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC740.11729089727536415No Hit
ACAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC720.11412087302467863No Hit
TCATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC720.11412087302467863No Hit
CATTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC710.11253586089933587No Hit
AACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC700.11095084877399312No Hit
TAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC700.11095084877399312No Hit
ATAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC680.1077808245233076No Hit
TTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC680.1077808245233076No Hit
TAAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC670.10619581239796484No Hit
TTGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC660.1046108002726221No Hit
CCACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC660.1046108002726221No Hit
GTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC660.1046108002726221No Hit
TTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC640.10144077602193657No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAACCT650.0295.000031
GGTCCTA1300.0295.000032
GAGCCTA650.0295.000032
TCTCCTA650.0295.000032
TGATCCT255.2568794E-10295.01
TCGCCTA900.0295.02
CCTTCCT157.2080165E-6295.01
GCCCCTA950.0295.02
AATCCTA1650.0295.02
AGTCCTA1350.0295.02
AAATCCT350.0295.01
CGAACCT255.2568794E-10295.01
TCCGCCT450.0295.01
GTACAGG157.2080165E-6295.0295
CAGCCTA950.0295.02
CCTAGGG157.2080165E-6295.05
TTAGCCT255.2568794E-10295.01
GAGTCCT255.2568794E-10295.01
TAGACCT255.2568794E-10295.01
GGGTCCT303.6379788E-12295.01
Produced by FastQC (version 0.11.5)