FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491011

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491011
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56277
Sequences flagged as poor quality0
Sequence length301
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1000.17769248538479307No Hit
TCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC950.1688078611155534No Hit
TACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC930.16525401140785756No Hit
TTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC880.1563693871386179No Hit
ATCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC860.15281553743092205No Hit
ACAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC840.14926168772322618No Hit
GTTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC830.14748476286937826No Hit
CTCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC820.1457078380155303No Hit
TTGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC810.14393091316168238No Hit
TTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC810.14393091316168238No Hit
TCATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC810.14393091316168238No Hit
ATCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC800.14215398830783446No Hit
TCACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC780.1386001386001386No Hit
ATATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC780.1386001386001386No Hit
CAAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC770.13682321374629067No Hit
CTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC770.13682321374629067No Hit
CCATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC770.13682321374629067No Hit
CTTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC760.13504628889244275No Hit
GCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC730.12971551433089895No Hit
ATAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC730.12971551433089895No Hit
TTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC730.12971551433089895No Hit
CAGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC730.12971551433089895No Hit
CCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC730.12971551433089895No Hit
ACCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC720.127938589477051No Hit
TAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC720.127938589477051No Hit
ATGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC720.127938589477051No Hit
TGCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC720.127938589477051No Hit
CACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC710.12616166462320308No Hit
CAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC710.12616166462320308No Hit
CACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC710.12616166462320308No Hit
TTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC710.12616166462320308No Hit
ATGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC710.12616166462320308No Hit
CGAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC700.12438473976935514No Hit
TCCCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC700.12438473976935514No Hit
ATTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC700.12438473976935514No Hit
TACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC700.12438473976935514No Hit
GTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC700.12438473976935514No Hit
TTACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC690.12260781491550722No Hit
AGCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC690.12260781491550722No Hit
TTTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC690.12260781491550722No Hit
CTTCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC690.12260781491550722No Hit
TCTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC680.1208308900616593No Hit
ATTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC680.1208308900616593No Hit
TCGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC680.1208308900616593No Hit
TCGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC680.1208308900616593No Hit
CTGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC670.11905396520781135No Hit
GGAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC670.11905396520781135No Hit
GAGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC660.11727704035396343No Hit
CAGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC660.11727704035396343No Hit
ACACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC660.11727704035396343No Hit
GTGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC660.11727704035396343No Hit
CCCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC660.11727704035396343No Hit
AACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC650.1155001155001155No Hit
GAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC640.11372319064626756No Hit
TAGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC640.11372319064626756No Hit
TCCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC640.11372319064626756No Hit
CATACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC640.11372319064626756No Hit
CCAGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC640.11372319064626756No Hit
CTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC640.11372319064626756No Hit
TATACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC640.11372319064626756No Hit
ACTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC640.11372319064626756No Hit
ATACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC630.11194626579241963No Hit
AATTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC630.11194626579241963No Hit
GGATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC630.11194626579241963No Hit
TTCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC630.11194626579241963No Hit
GTCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC630.11194626579241963No Hit
AGAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC630.11194626579241963No Hit
GGGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC620.11016934093857171No Hit
TGAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC620.11016934093857171No Hit
ACATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC620.11016934093857171No Hit
GTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC620.11016934093857171No Hit
AACCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC620.11016934093857171No Hit
ACGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC610.10839241608472379No Hit
TCCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC610.10839241608472379No Hit
GGGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC610.10839241608472379No Hit
AAGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC610.10839241608472379No Hit
TGTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC610.10839241608472379No Hit
TGGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC610.10839241608472379No Hit
TGGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC610.10839241608472379No Hit
ACCCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC600.10661549123087584No Hit
CGGCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC600.10661549123087584No Hit
CTACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC600.10661549123087584No Hit
TCTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC600.10661549123087584No Hit
AACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC600.10661549123087584No Hit
AATACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC600.10661549123087584No Hit
TCTCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC600.10661549123087584No Hit
TTCCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC600.10661549123087584No Hit
TTGCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC600.10661549123087584No Hit
GAAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC600.10661549123087584No Hit
ACTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC590.10483856637702792No Hit
GCACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC590.10483856637702792No Hit
TTGGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC590.10483856637702792No Hit
GGCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC590.10483856637702792No Hit
CTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC590.10483856637702792No Hit
CATTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC590.10483856637702792No Hit
GGTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC590.10483856637702792No Hit
GTCCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC580.10306164152318No Hit
TTTCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC580.10306164152318No Hit
TACCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC580.10306164152318No Hit
GTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC580.10306164152318No Hit
AAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC580.10306164152318No Hit
CCTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC570.10128471666933204No Hit
GGGCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC570.10128471666933204No Hit
GAGCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC570.10128471666933204No Hit
GCCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC570.10128471666933204No Hit
TAGCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC570.10128471666933204No Hit
AAAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC570.10128471666933204No Hit
CTCCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC570.10128471666933204No Hit
CCGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC570.10128471666933204No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGGA1150.0295.000036
TCGCCTA700.0295.02
ATCTCCT255.2386895E-10295.01
CTATCCT303.6379788E-12295.01
AGCCCTA850.0295.02
GAGTCCT255.2386895E-10295.01
TAGACCT255.2386895E-10295.01
ATAGCCT157.2038874E-6295.01
CGACCTA850.0295.02
CGCGCCT255.2386895E-10295.01
GGATCCT303.6379788E-12295.01
TGAACCT350.0295.01
GCGCCTA750.0295.02
GCACCTA950.0295.02
CGCCTAC3100.0295.03
TACGCCT303.6379788E-12295.01
GTCACCT157.2038874E-6295.01
TCGTCCT255.2386895E-10295.01
GTTCCTA700.0295.02
GAGCCTA600.0295.02
Produced by FastQC (version 0.11.5)