FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491021

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491021
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76972
Sequences flagged as poor quality0
Sequence length301
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC900.11692563529595178No Hit
CTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC890.11562646157044121No Hit
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC890.11562646157044121No Hit
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC870.11302811411942006No Hit
TCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC770.10003637686431431No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCTA900.0295.02
GCCCCCT108.449712E-4295.01
CCCCTAG108.449712E-4295.03
CGAACCT108.449712E-4295.01
AGCCCTA1050.0295.02
CTCCCTA850.0295.02
TCCTCGG108.449712E-4295.04
GCGCCCT400.0295.01
TACGGCG206.1476385E-8295.07
CGTTCCT255.2568794E-10295.01
TAGCCCT450.0295.01
GCACCCT206.1476385E-8295.01
CACCTAG108.449712E-4295.03
TTCCCTA1100.0295.02
AGTGCCT206.1476385E-8295.01
ACGGCGG206.1476385E-8295.08
ATCCCCT206.1476385E-8295.01
CCCCCCT206.1476385E-8295.01
GTTCCCT400.0295.01
CTACCTA1050.0295.02
Produced by FastQC (version 0.11.5)